| Project/Area Number |
24659641
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
TAKAMIZAWA Keiichi 独立行政法人国立循環器病研究センター, 研究所, 研究員 (10163312)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Yasuhide 独立行政法人国立循環器病研究センター, 研究所, 室長 (50250262)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 心臓弁 / 再生医療 / 組織工学 / 移植 / 大動脈弁 / 自己組織 / 生体内組織工学 |
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| Research Abstract |
A 3D printer, which can easily reproduce the 3D-shape and size of native heart valves, was used to produce the molds for a tri-leaflet, valved conduit with a sinus of Valsalva. Biovalves were obtained from completely autologous connective tissue, containing collagen and fibroblasts, within 2 months following the subcutaneous embedding of the molds. In vitro evaluation, using a pulsatile circulation circuit, showed excellent valvular function with a durability of at least 10 days. The Biovalves were implanted in goats through an apicoaortic bypass procedure. Smooth movement of the leaflets with minimal regurgitation were observed. After 1 month of implantation, smooth white leaflets were observed with minimal thrombus formation. Functional, autologous, 3D-shaped heart valves with clinical application potential were formed following in-body embedding of specially designed molds.
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