Basic research of anti-malignant tumor effect using fullerene particles and neutron capture reaction.

• Project/Area Number: 24659643
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: University of Tsukuba
• Principal Investigator: MATSUMURA Akira 筑波大学, 副学長 (90241819)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Fumiyo 筑波大学, 医学医療系, 助教 (30261811) ; KUMADA Hiroaki 筑波大学, 医学医療系, 准教授 (30354913) ; YAMAMOTO Tetsuya 筑波大学, 医学医療系, 講師 (30375505) ; NAKAI Kei 筑波大学, 医学医療系, 講師 (50436284)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: フラーレン / ガドリニウム / 中性子捕捉療法 / ホウ素 / 中性子

Outline of Final Research Achievements

We aimed at securing sufficient concentrations of Gadolinium in neutron capture therapy (BNCT) by developing a new drug delivery system using fullerene. We have designed and developed a novel particle that consists of C82 fullerene including Gadolinium. The particles are designed for having EPR effect and adhesive property by sizing and surface modification. A trial to study the safety and intra-tumoral gadolinium distribution using micro-Particle Induced X-ray Emission were performed. Cells were cultured with Gd-DTPA containing medium, and neutron irradiations revealed the radiobiological effect of neutron capture effect.

Research Products

• [Journal Article] ntra-tumor Distribution of Metallofullerene Using Micro-particle induced X-ray Emission (PIXE) (2014)
  • Author(s): Y. Yamamoto, T. Yamamoto, Y. Horiguchi, K. Endo, M. Shirakawa, T. Miyakawa , A. Matsumura H. Satoh, M. Koka, K. Nakai, Y. Nagasaki, A. Matsumura
  • Journal Title: Applied Radiation and isotope Volume: 88 Pages: 114-117
  • DOI: 10.1016/j.apradiso.2013.12.037
  • Peer Reviewed / Open Access
• [Presentation] ➢Additive effect of BPA and Gd-DTPA for application in accelerator-based neutron source (2014)
  • Author(s): F. Yoshida , K. Nakai, T. Yamamoto, A. Zaboronok, A. Matsumura
  • Organizer: 16th international congress of neutron capture therapy
  • Place of Presentation: Helsinki, Finland
  • Year and Date: 2014-06-14 – 2014-06-19
• [Presentation] ➢Application of micro-PIXE/PIGE technology to boron concentration analysis (2014)
  • Author(s): Kei Nakai, F. Yoshida, E. Okamoto, T. Sato, M. Koka, Y. Yamamoto,T.Yamamoto,A. Matsumura
  • Organizer: 16th international congress of neutron capture therapy
  • Place of Presentation: Helsinki, Finland
  • Year and Date: 2014-06-14 – 2014-06-19
• [Presentation] Newly designed boron-containing nanoparticle for high performance neutron capture therapeutics. (2013)
  • Author(s): Gao Z, Horiguchi Y, Nakai K, Matsumura A< Suzuki M, Ono Koji, Nagasaki Y
  • Organizer: バイオマテリアルシンポジウム
  • Place of Presentation: 東工大
• [Presentation] Micro-PIXE/PIGE法を用いた培養細胞におけるホウ素・ガドリニウム動態の検討． (2013)
  • Author(s): 13山本陽平，中井 啓，白川 真，吉田文代，佐藤隆博，江夏昌志，山本哲哉，松村 明
  • Organizer: 第10回 日本中性子捕捉療法学会
  • Place of Presentation: 岡山
• [Presentation] Micro-PIXE/PIGE法を用いた培養細胞におけるホウ素・ガドリニウム動態-中性子捕捉療法に向けた基礎的検討- (2013)
  • Author(s): 山本陽平，中井 啓，高 振宇，松村 明，江夏昌志，佐藤隆博．
  • Organizer: 第８回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎
• [Presentation] Metallofullerene Distribution Using Micro-particle induced X-ray emission for Gadolinium Neutron Capture Therapy (2012)
  • Author(s): Yohei Yamamoto, Kei Nakai, Yukichi Horiguchi, Kiyoshi Endo, Makoto Shirakawa, Takahiro Satoh, Masaaki Koka, Tetsuya Yamamoto, Yukio Nagasaki, Akira Matsumura
  • Organizer: 15th international congress of neutron capture therapy
  • Place of Presentation: Tsukuba
• [Book] TEXTBOOK for BNCT: Feasibility for Intramedullary Spinal Glioma (pp 407-415) (2013)
  • Author(s): Kei Nakai and Akira Matsumura
  • Total Pages: 553
  • Publisher: Springer-Verlag