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Mechanism of neruonal differention of pluripotent mesenchymal stem cells by intracullular delivery of BC-box motif peptides

Research Project

Project/Area Number 24659655
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cerebral neurosurgery
Research InstitutionYokohama City University

Principal Investigator

KANNO Hiroshi  横浜市立大学, 医学(系)研究科(研究院), 客員准教授 (40244496)

Co-Investigator(Kenkyū-buntansha) ITO Norihiko  鳥取大学, 農学部, 准教授 (80264654)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords多能性間葉系幹細胞 / BC-boxモチーフペプチド / 神経分化誘導 / 神経再生医療 / 多能性体性幹細胞 / 機能性ペプチド / 神経分化ドメイン / BCボックス蛋白 / 多能性幹細胞 / BCボックスモチーフ / ペプチド
Outline of Final Research Achievements

In this study we used neural stem cells and skin-derived mesenchymal stem cells as pluripotent somatic stem cells to neuronal lineage.BC-box proteins-derived functional peptides correspoding to BC-box motif strucre[(A,P,S,T)LXXX (A,C) XXX(A,I,L,V)], showing induction activity of neuronal differentiation, are delivered to those somatic stem cells. Different BC-box motif peptides induced to differentiate different type of neuron, but two kinds of somatic stem cells differentiated to same neurons. This mechanism of neuronal differentiation is suggested to be related to blocking of binding between elongin BC & A, and inhibition of JAK2/Stat3, but is under investigation. From these results, it is considered that this study will contribute to neuronal regenerative medicine using somatic stem cells differentiated to useful neurons.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (3 results)

  • [Journal Article] Role of the von Hippel-Lindau tumor suppressor protein during neuronal differentiation of somatic stem cells and its application to neuronal regeneration. A review.2014

    • Author(s)
      Kanno H, Kubo A, Higashida T
    • Journal Title

      Journal of Translational Medicine and Epidemiology

      Volume: 2(1) Pages: 1013-1013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] S: Pathological and clinical features and management of central nervous system hemangioblastomas in von hippel-lindau disease.2014

    • Author(s)
      Kanno H, Kobayashi N, Nakanowatari
    • Journal Title

      Journal of Kidney Cancer and VHL) 1(4):46-55, 2014.

      Volume: 1(4) Issue: 4 Pages: 46-55

    • DOI

      10.15586/jkcvhl.2014.12

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells2013

    • Author(s)
      Kanno H, Kubo A, Yoshizumi T, Mikami T, Maegawa J
    • Journal Title

      Int J Mol Sci

      Volume: 14(5) Issue: 5 Pages: 9604-9617

    • DOI

      10.3390/ijms14059604

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The VHL tumor suppressor protein regulates tumorigenicity of U87-derived glioma stem-like cells by inhibiting the JAK/STAT signaling pathway2013

    • Author(s)
      Kanno H, Sato H, Yokoyama TA, Yoshizumi T, Yamada S
    • Journal Title

      Int J Oncol

      Volume: 42(3) Issue: 3 Pages: 881-6

    • DOI

      10.3892/ijo.2013.1773

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regenerative therapy for neuronal diseases with transplantation of somatic stem cells2013

    • Author(s)
      Kanno H
    • Journal Title

      World Journal of Stem Cells

      Volume: 5 Pages: 163-171

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Therapeutic strategies for central nervous system hemangioblastomas in von Hippel-Lindau disease.2014

    • Author(s)
      Kanno H, Nakanowatari S
    • Organizer
      11 th International VHL Symposium
    • Place of Presentation
      Madrid, Spain
    • Year and Date
      2014-10-23 – 2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Isolation of multipotent nestin-expressing hair follicle stem cells derived from epidermis of elderly humans and TAT-VHLpeptide-mediated neuronal differentiation of these cells2013

    • Author(s)
      Kanno H, Kubo A, Yoshizumi T, Mikami T, Maegawa J
    • Organizer
      Annual Meeting 2013, Society for Neuroscience
    • Place of Presentation
      San Diego, Calfornia, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Identification of a neural Induction domain in BC-box proteins and neural induction of somatic stem cells by transfer of its domain peptide2012

    • Author(s)
      Kanno H, Kubo A, Higashida T, Ito N
    • Organizer
      The Annual Meeting of International Society for Stem Cell Research
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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