| Project/Area Number |
24659664
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INUI Hiroshi 東京大学, 医学部附属病院, 助教 (60583119)
SAITO Taku 東京大学, 医学部附属病院, 助教 (30456107)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 軟骨 / 軟骨細胞 |
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| Research Abstract |
We carried out research on a new molecule named ZNF449 and focused on its function in chondrocytes. We started by investigating the expression pattern in the growth plate and found that it was expressed ubiquitously but mainly in the prehypertrophic region. In vitro experiments, such as promoter analysis and ATDC5 differentiation suggested involvement in the process of hypertrophy. Therefore, we created a knockout mouse and assessed its phenotype. Unfortunately, it did not show any growth retardation, nor did it show any difference when we created a surgically induced osteoarthritis model. We also analysed other mesenchymal tissues such as muscles and bones but we could not find any apparent difference between the wild-type mice.
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