Development of a biofunctional scaffold using nanocarbon fiber sheets for bone regenaration

• Project/Area Number: 24659670
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Shinshu University
• Principal Investigator: SAITO Naoto 信州大学, 学術研究院保健学系, 教授 (80283258)
• Co-Investigator(Kenkyū-buntansha): KATO Hiroyuki 信州大学, 学術研究院医学系, 教授 (40204490) ; KIM Yoong Ahm 信州大学, 工学部, 准教授 (70362100)
• Co-Investigator(Renkei-kenkyūsha): USUI Yuki 信州大学, 医学部附属病院, 特任研究員 (00467169) ; HANIU Hisao 信州大学, 学術研究院医学系, 講師 (30252050) ; SHIMIZU Masayuki 信州大学, 医学部附属病院, 助教(特定雇用) (40467171) ; TAKANASHI Seiji 信州大学, 医学部附属病院, 医員 (70596783) ; OKAMOTO Masanori 信州大学, 医学部附属病院, 助教(診療) (50596781) ; KOBAYASHI Shinsuke 信州大学, 医学部附属病院, 医員 (40624705) ; TANAKA Manabu 信州大学, 医学部附属病院, 医員 (30723069)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 骨組織再生 / カーボンファイバー / scaffold / 生体安全性 / 再生医療 / 足場材 / ナノカーボンファイバー / 骨髄細胞

Outline of Final Research Achievements

We recently developed nanocarbon fiber sheets (NCFSs) made from electrospun nanosized carbon fiber. Here, we investigated the possibility of using NCFSs as a scaffold for bone regeneration by evaluating bone tissue compatibility and osteogenesis.
NCFS scaffolding enabled good bone regeneration of skull defects in mice through interactions with the bone morphogenic protein rhBMP-2. In proliferation trials, osteoblasts showed good compatibility with NCFS, and ALP activity in culture assays with calcification medium indicated elevated differentiation of osteoblasts. Based on these findings, NCFSs may have potential bioapplications as a scaffold for bone regeneration.

Research Products

• [Journal Article] Safe clinical use of carbon nanotubes as innovative biomaterials (2014)
  • Author(s): Saito N, Haniu H, Usui Y, Aoki K, Hara K, Takanashi S, Shimizu M, Narita N, Okamoto M, Kobayashi S, Nomura H, Kato H, Nishimura N, Taruta S, Endo M
  • Journal Title: Chem Rev Volume: 114 Issue: 11 Pages: 6040-6079
  • DOI: 10.1021/cr400341h
  • Peer Reviewed / Open Access
• [Journal Article] Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells (2014)
  • Author(s): Haniu H, Saito N, Matsuda N, Tsukahara T, Usui Y, Maruyama K, Takanashi S, Aoki K, Kobayashi S, Nomura H, Tanaka M, Okamoto M, Kato H
  • Journal Title: Int J Nanomed Volume: ９ Pages: 1979-1989
  • DOI: 10.2147/ijn.s58661
  • NAID: 120007110603
  • Peer Reviewed / Open Access
• [Journal Article] Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response (2013)
  • Author(s): Haniu H, Saito N, Matsuda Y, Tsukahara T, Maruyama K, Usui Y, Aoki K, Takanashi S, Kobayashi S, Nomura H, Okamoto M, Shimizu M, Kato H
  • Journal Title: Toxicol in Vitro Volume: 27 Issue: 6 Pages: 1679-1685
  • DOI: 10.1016/j.tiv.2013.04.012
  • NAID: 120007110519
  • Peer Reviewed
• [Presentation] Strength improvement and tissue compatibility of multi-walled carbon nanotubes-polyethylene complex for prosthetic joint. (2013)
  • Author(s): Aoki K, Takanashi S, Shimizu M, Haniu H, Kato H, Usui Y, Nishi K, Noguchi T, Nishimura N, Saito N.
  • Organizer: 25th European Conference on Biomaterials.
  • Place of Presentation: Madrid, Spain.
• [Presentation] Carcinogenicity evaluation of carbon nanotubes in rasH2 mice. (2013)
  • Author(s): Takanashi S, Haniu H, Usui U, Aoki K, Okamoto M, Kobayashi S, Nomura H, Tanaka M, Kato H, Saito N.
  • Organizer: The 6th International Symposium on Nanotechnology, Occupational and Environmental Health.
  • Place of Presentation: Nagoya, Japan.
• [Presentation] Endocytosis of MWCNT in BEAS-2B cells are affected by the culture medium type. (2013)
  • Author(s): Haniu H, Saito N, Matsuda N, Maruyama K, Tsukahara T, Usui Y, Takanashi S, Aoki K, Kobayashi S, Nomura H, Okamoto M, Shimizu M, Kato H.
  • Organizer: 6th International Symposium on Nanotechnology, Occupational and Environmental Health.
  • Place of Presentation: Nagoya, Japan.