| Project/Area Number |
24659676
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kobe University |
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Principal Investigator |
KUROSAKA Masahiro 神戸大学, 医学(系)研究科(研究院), 教授 (70170115)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Tomoyuki 神戸大学, 医学部附属病院, 助教 (50546588)
MATSUSHITA Takehiko 神戸大学, 医学部附属病院, 助教 (40467650)
KURODA Ryosuke 神戸大学, 医学(系)研究科(研究院), 准教授 (80379362)
KUBO Seiji 神戸大学, 医学部附属病院, 助教 (30452494)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 前十字靭帯 / Statin / 再生医療 / スタチン |
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| Research Abstract |
The aim of this study was to test the hypothesis that enhanced angiogenesis and osteogenesis by locally applied simvastatin promotes tendon-bone healing after ACL reconstruction. Rabbits received ACL reconstruction with hamstring tendon graft and were implanted with either simvastatin-conjugated gelatin hydrogel or gelatin hydrogel alone in their bone tunnels. Histological and radiological analysis at week 2 demonstrated early tendon-bone healing with enhanced angiogenesis and osteogenesis in the simvastatin-treated group. Biomechanical testing at week 2 also demonstrated a significant increase in ultimate failure load in the simvastatin-treated group. This study suggested that local administration of low-dose simvastatin-conjugated gelatin hydrogel promotes the tendon-bone healing via its effect on both angiogenesis and osteogenesis at an early phase in a rabbit model, but does not affect biomechanical property in long-term after ACL reconstruction.
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