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Analysis of the TGF-b signaling in cartilage by nonlinear optics imaging combined with genetic engineering techniques

Research Project

Project/Area Number 24659680
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionEhime University

Principal Investigator

MIURA Hiromasa  愛媛大学, 医学(系)研究科(研究院), 教授 (10239189)

Co-Investigator(Renkei-kenkyūsha) IMAMURA Takeshi  愛媛大学, 大学院医学系研究科, 教授 (70264421)
HIKITA Atsuhiko  愛媛大学, 大学院医学系研究科, 准教授 (60443397)
IIMURA Tadahiro  愛媛大学, 大学院医学系研究科, 准教授 (20282775)
OSHIMA Yusuke  愛媛大学, 医学部附属病院, 助教 (10586639)
MIYAZAKI Tsuyoshi  東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究員 (50376480)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsTGF-β / 軟骨 / 軟骨変性 / 変形性関節症 / SHG / 2光子励起 / Smurf / 二光子励起 / 2光子励起顕微鏡
Research Abstract

Multi-photon microscopy enables to characterize and evaluate alternation of collagen matrix in vivo and in vitro based on second harmonic generation (SHG) signal. We performed SHG imaging of the cartilage degeneration induced by enzyme treatment. In the result, changes in SHG signal intensity were observed in the experimental samples. We also established an animal osteoarthritis model by syndesmectomy using H2B-GFP line mice. We successfully evaluated over time the changes in cartilage matrix and chondrocytes by SHG and two-photon excited fluorescence. Furthermore, the articular cartilages of aged mice were observed as the same manner. In comparison with those of young mice, it was possible to evaluate them as spontaneous knee osteoarthritis. Our technique that combined genetic engineering and nonlinear interaction between the molecules and photons to approach the mechanism of cartilage degeneration is promising as a translational research leading to clinical application.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2014 2013

All Presentation (12 results)

  • [Presentation] Evaluation of cartilage based on second harmonic generation microscopy2014

    • Author(s)
      Hiroshi Kiyomatsu, Yusuke Oshima, Atsuhiko Hikita, Tadahiro Iimura, Takashi Saito, Tsuyoshi Miyazaki, Hiromasa Miura, Takeshi Imamura
    • Organizer
      American Society for Bone and Mineral Research Annual Meeting 2014
    • Related Report
      2013 Final Research Report
  • [Presentation] Evaluation of osteoarthritis based on second harmonic generation (SHG) imaging2014

    • Author(s)
      清松悠、大嶋佑介、疋田温彦、三浦裕正、今村健志
    • Organizer
      第6回大学院専攻研究発表会
    • Place of Presentation
      東温市
    • Related Report
      2013 Final Research Report
  • [Presentation] Evaluation of osteoarthritis based on second harmonic generation (SHG) imaging2014

    • Author(s)
      清松悠、大嶋佑介、疋田温彦、三浦裕正、今村健志
    • Organizer
      第4回Vivid Workshop
    • Place of Presentation
      金沢市
    • Related Report
      2013 Final Research Report
  • [Presentation] Evaluation of osteoarthritis based on second harmonic generation (SHG) imaging2014

    • Author(s)
      清松悠、大嶋佑介、疋田温彦、三浦裕正、今村健志
    • Organizer
      第4回 Vivid Workshop
    • Place of Presentation
      金沢市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evaluation of osteoarthritis based on second harmonic generation (SHG) imaging2014

    • Author(s)
      清松悠、大嶋佑介、疋田温彦、三浦裕正、今村健志
    • Organizer
      第6回 大学院専攻研究発表会
    • Place of Presentation
      東温市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evaluation of cartilage based on second harmonic generation microscopy2014

    • Author(s)
      H. Kiyomatsu, Y.Oshima, A.Hikita, T.Iimura, T.Saito, T.Miyazaki, H.Miura, T.Imamura
    • Organizer
      American Society for Bone and Mineral Research Annual Meeting 2014
    • Place of Presentation
      Houston, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evaluation of osteoarthritis based on SHG imaging TGF-βfamily2013

    • Author(s)
      Hiroshi Kiyomatsu, Yusuke Oshima, Atsuhiko Hikita, Hiromasa Miura, Takeshi Imamura
    • Organizer
      Signal Network and Imaging Live
    • Place of Presentation
      Matsuyama
    • Related Report
      2013 Final Research Report
  • [Presentation] Evaluation of osteoarthritis based on second harmonic generation microscopy2013

    • Author(s)
      Hiroshi Kiyomatsu, Yusuke Oshima, Atsuhiko Hikita, Hiromasa Miura, Takeshi Imamura
    • Organizer
      American Society for Bone and Mineral Research Annual Meeting 2013
    • Place of Presentation
      Baltimore, USA
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Evaluation degeneration of articular cartilage in mice based on second harmonic generation microscopy2013

    • Author(s)
      Hiroshi Kiyomatsu, Yusuke Oshima, Atsuhiko Hikita, Hiromasa Miura, Takeshi Imamura
    • Organizer
      Conference on Laser Surgery and Medicine
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Final Research Report
  • [Presentation] Evaluation of articular cartilage degeneration in mice based on second harmonic generation microscopy2013

    • Author(s)
      Hiroshi Kiyomatsu.,Yusuke Oshima., Atsuhiko Hikita., Hiromasa Miura.,Takeshi Imamura.
    • Organizer
      Conference on Laser Surgery and Medicine 2013
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report 2012 Research-status Report
  • [Presentation] Evaluation of osteoarthritis based on SHG imaging2013

    • Author(s)
      Hiroshi Kiyomatsu.,Yusuke Oshima., Atsuhiko Hikita., Hiromasa Miura.,Takeshi Imamura.
    • Organizer
      TGF-β family Signal Network and Live Imaging
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 非線形光学技術を用いた変形性関節症の評価法の確立2013

    • Author(s)
      清松悠、大嶋佑介、疋田温彦、三浦裕正、今村健志
    • Organizer
      Orthopedic Research Club 2013
    • Place of Presentation
      木更津市
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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