Spinal cord regeneration treatment by grafting foliiculo-stellate cells

• Project/Area Number: 24659681
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya City University
• Principal Investigator: WADA Ikuo 名古屋市立大学, 医学(系)研究科(研究院), 教授 (70182970)
• Co-Investigator(Kenkyū-buntansha): WAKABAYASHI Kenjiro 名古屋市立大学, 大学院医学研究科, 助教 (20418867) ; SAKUMA Eisuke 名古屋市立大学, 大学院医学研究科, 講師 (90295585)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 再生医療 / 下垂体 / 脊髄損傷 / 移植治療 / ギャップジャンクション / 再生医学 / 体性幹細胞 / 電気生理学 / 細胞移植 / 細胞間連絡 / 電子顕微鏡

Outline of Final Research Achievements

The preservation of gap junctions between folliculo-stellate cells are mainly dependent on the testosterone from both testes and adrenal glands in adult male rats (Microsc Res Tech. 2012). The effects of leptin over the gap junction formation within the anterior pituitary glands was directly mediated by interaction with the functional leptin receptor over the folliculo-stellate cells (Microsc Res Tech. 2014). We investigated a detailed distribution and postnatal changes of the S-100 protein-positive/folliculo-stellate cells and their relationship with LH-RH nerve elements in the pars-tuberalis of pituitary gland and showed their participation in the function (Tissue Cell. 2014). The responsiveness to LHRH has a long-term refractoriness in adult rats, and that the physiological LHRH surge may be clocked in the morning due to the gap junction (Endocrinology. 2013). These findings are important for the spinal cord regeneration treatment by grafting foliiculo-stellate cells.

Research Products

• [Journal Article] Intercellular communications within the rat anterior pituitary. XVI: postnatal changes of distribution of S-100 protein positive cells, connexin 43 and LH-RH positive sites in the pars tuberalis of the rat pituitary gland. An immunohistochemical and electron microscopic study. (2014)
  • Author(s): Wada I, Sakuma E, Shirasawa N, Wakabayashi K, Otsuka T, Hattori K, Yashiro T, Herbert DC, Soji T.
  • Journal Title: Tissue Cell Volume: 46 Pages: 33-39
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The changes of gap junctions between pituitary folliculo-stellate cells during the postnatal development of Zucker fatty and lean rats. (2014)
  • Author(s): Sakuma E, Wada I, Soji T, Wakabayashi K, Otsuka T, Herbert DC.
  • Journal Title: Microsc Res Tech Volume: 77 Pages: 31-36
  • Peer Reviewed / Open Access
• [Journal Article] The changes of gap junctions between pituitary folliculo-stellate cells during the postnatal development of Zucker fatty and lean rats (2014)
  • Author(s): Wada, Sakuma, Wakabayasi et al.
  • Journal Title: Microsc Res Tech Volume: 77 Pages: 31-36
  • Peer Reviewed
• [Journal Article] Intercellular communication within the rat anterior pituitary gland. XV. Properties of spontaneous and LHRH-induced Ca2+ transients in the transitional zone of the rat anterior pituitary in situ (2013)
  • Author(s): Wada et al.
  • Journal Title: Endocrinology Volume: 154 Pages: 400-409
  • Peer Reviewed
• [Journal Article] Intercellular communication within the rat anterior pituitary gland. XV. Properties of spontaneous and LHRH-induced Ca2+ transients in the transitional zone of the rat anterior pituitary in situ. (2013)
  • Author(s): Hattori K, Shirasawa N, Suzuki H, Otsuka T, Wada I, Yashiro T, Herbert DC, Soji T, Hashitani H.
  • Journal Title: Endocrinology Volume: 154(1) Pages: 400-409
  • Peer Reviewed
• [Journal Article] Involvement of the adrenal glands and testis in gap junction formation via testosterone within the male rat anterior pituitary gland. (2012)
  • Author(s): Sakuma E, Wada I, Otsuka T, Wakabayashi K, Ito K, Soji T, Herbert DC.
  • Journal Title: Microsc Res Tech. Volume: 75(12) Pages: 1632-1638
  • Peer Reviewed
• [Presentation] Postnatal Changes of Distribution of S-100 Protein Positive Cells, Connexin 43 and LH-RH Positive Sites in the Pars Tuberalis of the Rat Pituitary Gland (2015)
  • Author(s): 佐久間英輔 、和田郁雄 、白澤信行 、若林健二郎 、大塚隆信 、植木孝俊
  • Organizer: 第120回日本解剖学会全国学術集会・第92回生理学会大会・合同大会
  • Place of Presentation: 兵庫県 神戸国際会議場
  • Year and Date: 2015-03-22
• [Presentation] 遺伝性肥満ラットZucker fatty に見いだされた 濾胞星細胞間のギャップジャンクションの形成不全 (2014)
  • Author(s): Wada, Sakuma et al.
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学キャンパス（栃木県）
• [Presentation] 下垂体前葉内における濾胞星細胞を介した新たな情報伝達系 (2013)
  • Author(s): 佐久間英輔、馬渕良生、曽爾彊、和田郁雄
  • Organizer: 第１１８回 日本解剖学会総会
  • Place of Presentation: サンポート高松(香川県）