Reclamation of a novel OA therapy by the control of Myostatin

• Project/Area Number: 24659683
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: ARAI Yuji 京都府立医科大学, 医学(系)研究科(研究院), 講師 (50347449)
• Co-Investigator(Kenkyū-buntansha): KISHIDA Tsunao 京都府立医科大学, 医学研究科, 講師 (00370205) ; KUBO Toshikazu 京都府立医科大学, 医学研究科, 教授 (20178031) ; TERAUCHI Ryu 京都府立医科大学, 医学研究科, 講師 (20575154)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ミオスタチン / hiPS細胞 / 筋芽細胞 / siRNA / 筋力訓練 / 変形性関節症

Research Abstract

Myostatin negatively regulates proliferation of satellite cells that are tissue resident stem cells in the skeletal muscle, as well as differentiation of myoblasts into myotubes. Myostatin is crucially involved in pathogenesis of some diseases including cachexia, while recent studies have clarified its important roles in the control of carbohydrate and lipid metabolism. Blocking of Myostatin signal may provide novel therapeutic modality to prevent and improve atrophic conditions in cachexia and sarcopenia, and to control obesity and diabetes as well.

Research Products

• [Journal Article] Myostatin acts as an autocrine/paracrine negative regulator in myoblast differentiation from human induced pluripotent stem cells (2013)
  • Author(s): Fei Gao, Tsunao Kishida, Akika Ejima, Satoshi Gojo, Osam Mazda
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 431 Pages: 309-314
  • Peer Reviewed
• [Journal Article] Myostatin acts as an autocrine/paracrine negative regulator in myoblast differentiation from human induced pluripotent stem cells. (2013)
  • Author(s): Gao F, Kishida T, Ejima A, Gojo S, Mazda O.
  • Journal Title: Biochem Biophys Res Commun. Volume: 431(2) Issue: 2 Pages: 309-14
  • DOI: 10.1016/j.bbrc.2012.12.105
  • Peer Reviewed
• [Presentation] Anti-inflammatory effects of lansoprazole by suppressing ROS production in murine macrophage RAW 264.7 cells (2014)
  • Author(s): Shohei Ichimaru, Shuji Nakagawa, Yuji Arai, Shinji Tsuchida, Hiroaki Inoue, Tomohiro Matsuki, Yasuo Mikami, Takumi Ikeda, Ryo Oda, Osam Mazda, Toshikazu Kubo
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: New Orleans
• [Presentation] The expression levels of connexin 43 in human synovium of rheumatoid arthritis (2014)
  • Author(s): Tomohiro Matsuki, Yuji Arai, Shinji Tsuchida, Ryu Terauchi, Hiroaki Inoue, Shuji Nakagawa, Atsuo Inoue, Kuniaki Honjo, Toru Morihara, Toshiharu Shirai, Osam Mazda, Toshikazu Kubo
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: New Orleans
• [Presentation] Sonoporation-mediated transduction of siena targeting TNF alpha ameliorated experimental arthritis (2014)
  • Author(s): Inoue H, Arai Y, Terauchi R, Nakagawa S, Saito M, Tsuchida S, Inoue A, Matsuki T, Morihara T, Shirai T, Mazda O, Kubo T
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: New Orleans
• [Presentation] ソノポレーションで導入したTNF-α特異的siRNAによる関節炎モデルの治療効果 (2013)
  • Author(s): 井上裕章,新井祐志,中川周士,寺内竜,土田真嗣,松木智宏,井上敦夫,齊藤正純,松田修,久保俊一
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉県
  • Year and Date: 2013-10-18
• [Presentation] 活性化マクロファージに対するROSを介したランソプラゾールの抗炎症効果の解析 (2013)
  • Author(s): 市丸昌平,中川周士,新井祐志,土田真嗣,井上裕章,松木智宏,久保俊一
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉県
  • Year and Date: 2013-10-17
• [Presentation] The roles of heat shock protein 70 on chondrocyte (2013)
  • Author(s): Matsuki T, Arai Y, Tsuchida S, Terauchi R, Inoue H, Nakagawa S, Inoue A, Mazda O, Kubo T
  • Organizer: 16th World Congress of the Osteoarthritis Research Society International (OARSI)
  • Place of Presentation: Philadelphia, U.S.A
  • Year and Date: 2013-04-18
• [Presentation] Effect of mechanical stress on the hypertrophic differentiation related gene expression in cultured chondrocytes (2013)
  • Author(s): Inoue H, Arai Y, Terauchi R, Nakagawa S, Saito M, Hiraoka N, Tsuchida S, Matsuki T, Mazda O, Kubo T
  • Organizer: 16th World Congress of the Osteoarthritis Research Society International (OARSI)
  • Place of Presentation: Philadelphia, U.S.A
  • Year and Date: 2013-04-18
• [Presentation] 関節リウマチ滑膜におけるconnexin43の発現 (2013)
  • Author(s): 松木智宏、新井祐志、土田真嗣、本城邦晃、中川周士、井上裕章、寺内竜、松田修、久保俊一
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
• [Presentation] 変形性関節症の最新療法 (2013)
  • Author(s): 新井祐志
  • Organizer: 第40回日本生体電気・物理刺激研究会
  • Place of Presentation: ウェスティン都ホテル京都