Project/Area Number |
24659685
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Orthopaedic surgery
|
Research Institution | Keio University |
Principal Investigator |
MATSUO Koichi 慶應義塾大学, 医学部, 教授 (40229422)
|
Co-Investigator(Kenkyū-buntansha) |
TAKADA Yasunari 慶應義塾大学, 医学部, 講師 (40407086)
|
Research Collaborator |
MATSUMOTO Tomohiro 東海大学, 医学部, 学生
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 骨代謝 / 石灰化 / 転写因子 / メダカ / 骨芽細胞 / ミネラリゼーション / 遺伝子 / 細胞・組織 / 発現制御 |
Research Abstract |
We generated transgenic medaka fish expressing mouse Fra-1 in osteoblasts to determine cell-autonomous function of Fra-1 in osteoblasts in vivo. Alizarin complexone staining and micro-computed tomography revealed that Fra-1 transgenic medaka showed lower bone mineral density than wild-type controls. These data suggest that Fra-1 expression in osteoblasts impairs bone mineralization not only in mice but also in medaka.
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