| Project/Area Number |
24659695
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
|
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| Research Institution | Kansai Medical University (2013)
Kyoto University (2012) |
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Principal Investigator |
HIROTA Kiichi 関西医科大学, 医学部, 准教授 (00283606)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 低酸素 / 腎臓 / HIF-1 / 代謝 / リプログラミング / 保護 / 解糖 |
|---|
| Research Abstract |
In order to established a novel method to protect kidney from ischemic injury, we explored an intervention by manipulate the transcription factor HIF-1 activity with the small molecular compound n-propyl gallate (nPG). We demonstrated that forced activation of hypoxia-inducible factor 1(HIF-1) by the exogenous small molecule n-propyl gallate (nPG) induced metabolic reprograming from cellular energy generation dependent on OXPHOS in mitochondria to glycolysis-dominant one in the primary cultured tubular and glomerular cells. Moreover, we indicated that nPG pretreatment induced HIF-1 activation and expression of glycolytic enzymes and conferred tolerance against hypoxia-induced cell death of cells derived from kidney.
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