Investigation for establishment for a novel intervention to protect kidney against hypoxic injury by inducing metabolic reprogramming by manipulating the transcription factor HIF-1 activity
| Project/Area Number |
24659695
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kansai Medical University (2013)
Kyoto University (2012) |
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Principal Investigator |
HIROTA Kiichi 関西医科大学, 医学部, 准教授 (00283606)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 低酸素 / 腎臓 / HIF-1 / 代謝 / リプログラミング / 保護 / 解糖 |
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| Research Abstract |
In order to established a novel method to protect kidney from ischemic injury, we explored an intervention by manipulate the transcription factor HIF-1 activity with the small molecular compound n-propyl gallate (nPG). We demonstrated that forced activation of hypoxia-inducible factor 1(HIF-1) by the exogenous small molecule n-propyl gallate (nPG) induced metabolic reprograming from cellular energy generation dependent on OXPHOS in mitochondria to glycolysis-dominant one in the primary cultured tubular and glomerular cells. Moreover, we indicated that nPG pretreatment induced HIF-1 activation and expression of glycolytic enzymes and conferred tolerance against hypoxia-induced cell death of cells derived from kidney.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] Involvement of decreased hypoxia-inducible factor 1 activity and resultant G(1)-S cell cycle transition in radioresistance of perinecrotic tumor cells2013
Author(s)
Zhu Y, Zhao T, Itasaka S, Zeng L, Yeom CJ, Hirota K, Suzuki K, Morinibu A, Shinomiya K, Ou G, Yoshimura M, Hiraoka M, Harada H
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Journal Title
Oncogene
Volume: 32
Pages: 2058-68
Related Report
Peer Reviewed
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[Journal Article] Involvement of decreased hypoxia-inducible factor 1 activity and resultant G(1)-S cell cycle transition in radioresistance of perinecrotic tumor cells2013
Author(s)
Y. Zhu, T. Zhao, S. Itasaka, L. Zeng, C.J. Yeom, K. Hirota, K. Suzuki, A. Morinibu, K. Shinomiya, G. Ou, M. Yoshimura, M. Hiraoka, H. Harada
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Journal Title
Oncogene
Volume: 32
Pages: 2058-2068
DOI
Related Report
Peer Reviewed
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[Journal Article] Cancer cells that survive radiation therapy acquire HIF-1 activity and translocate towards tumour blood vessels2012
Author(s)
Harada H, Inoue M, Itasaka S, Hirota K, Morinibu A, Shinomiya K, Zeng L, Ou G, Zhu Y, Yoshimura M, McKenna WG, Muschel RJ, Hiraoka M
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Journal Title
Nature communications
Volume: 3
Pages: 783-783
NAID
Related Report
Peer Reviewed
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[Journal Article] Cancer cells that survive radiation therapy acquire HIF-1 activity and translocate towards tumour blood vessels2012
Author(s)
H. Harada, M. Inoue, S. Itasaka, K. Hirota,A. Morinibu, K. Shinomiya, L. Zeng, G. Ou, Y. Zhu, M. Yoshimura, W.G. McKenna, R.J. Muschel, M. Hiraoka
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Journal Title
Nat Commun
Volume: 3
Pages: 783-783
DOI
NAID
Related Report
Peer Reviewed
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