| Project/Area Number |
24659701
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
ISEKI Yuzo 福島県立医科大学, 医学部, 助手 (60622313)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 芍薬甘草湯 / 芍薬 / 甘草 / ポタシウム電流 / ホールセルクランプ法 / H9ｃ２細胞 / H9c2細胞 / ポタシウムイオンチャネル |
|---|
| Research Abstract |
Shakuyakukanzoto is a commonly used for the treatment of acute pain associated with muscle cramp. In the present study using the whole-cell patch clamp technique, we examined effects of Shakuyakukanzoto on the ultra-rapidly activating slowly inactivating delayed rectifier K+ current (IKur) in H9c2 cells, that is a cell line derived from embryonic rat heart, and can differentiate into skeletal myocytes. Shakuyakukanzoto inhibited IKur in a concentration-dependent manner. The half-maximal concentration (IC50) was approximately 1.3 mg/ml and the Hill coefficient was 1.2. The order of potency of inhibiting IKur was Kanzo> Shakuyakukanzoto> Shakuyaku. Glycyrrhizin, which is a major component of Kanzo, had no inhibitory effect on IKur. Our result suggest that Shakuyakukanzoto may normalize intracellular and extracellular K+ balance by inhibiting IKur and reducing K+ efflux. This may be a part of the mechanism that Shakuyakukanzoto improving muscle pain.
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