| Project/Area Number |
24659720
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
OHNISHI Makoto 国立感染症研究所, 細菌第一部, 部長 (10233214)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 淋菌 / セフトリアキソン耐性 / 咽頭常在ナイセリア属菌 / 耐性菌 / ナイセリア属菌 / ペニシリン結合タンパク |
|---|
| Research Abstract |
Various kinds of antimicrobial-resistant pathogens have emerged. In addition to pathogens causing nosocomial infections, pathogens for community-acquired infections also have developed their antimicrobial-resistance.
n this project, we tried to understand the mode of emergence and acquisition of resistant gene. We analyzed total 107 commesal neisserial strains, which are speculated as a source of resistant genes for N. gonorrhoeae. We could detect ceftriaxone (CRO) resistant genes in the commesal strains. From the comparison of penA genes, newly identified penA from CRO-resistant commensal strains were similar (not identical) with penA (H041). Some unique amino acids observed in penA (H041) were shared with the CRO resistant strains. We designed real-time PCR assay system, which could detect penA (H041) specifically. We tried to detect CRO-resistant neisserial strains after CRO treatment for N. gonorrhoeae, but the resistant strains could not be emerged.
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