Establishment of whole ovarian frozen preservation of Macaca

• Project/Area Number: 24659729
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: AKIE Takebayashi 滋賀医科大学, 医学部, 助教 (00402735)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 卵巣凍結

Outline of Final Research Achievements

We performed experiment of mouse ovaries before beginning experiment of macaca ovaries. To make a study of thawing condition, we froze mouse ovaries and performed immunohistochemistry of frozen mouse ovaries. Then, we froze macaca ovaries with CAS. The ovaries are kept in liquid nitrogen. Now we adjust a schedule for autogenous transplantation.

Research Products

• [Journal Article] Subpopulations of Macrophages within Eutopic Endometrium of Endometriosis Patients (2015)
  • Author(s): kie Takebayashi, Fuminori Kimura, Yohei Kishi, Mitsuaki Ishida, Akimasa Takahashi, Akiyoshi Yamanaka, Di Wu, Luyi Zheng, Kentaro Takahashi, Hiroshi Suginami, Takashi Murakami
  • Journal Title: American Journal of Reproductive Immunology Volume: 73 Issue: 3 Pages: 221-231
  • DOI: 10.1111/aji.12331
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The association between endometriosis and chronic endometritis. (2014)
  • Author(s): Akie Takebayashi, Fuminori Kimura, Yohei Kishi, Mitsuaki Ishida, Akimasa Takahashi, Akiyoshi Yamanaka, Kentaro Takahashi, Hiroshi Suginami, Takashi Murakami
  • Journal Title: Plos one Volume: 9 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Comparison of the outcome of in vitro fertilization after laparoscopic laser ablation surgery versus laparoscopic cystectomy for endometrioma (2013)
  • Author(s): Akie Takebayashi, Shimizu Y, Akimasa Takahashi, Akiyoshi Yamanaka, Akiko Takashima, Fuminori Kiumura, Nobuyuki Kita, Kentaro Takahashi, Takashi Murakami.
  • Journal Title: Gynecology and minimally invasive therapy Volume: 2 Issue: 1 Pages: 27-29
  • DOI: 10.1016/j.gmit.2012.12.005
  • Peer Reviewed
• [Journal Article] Intake of Vinegar Beverage Is Associated with Restoration of Ovulatory Function in Women with Polycystic Ovary Syndrome (2013)
  • Author(s): Di Wu, Fuminori Kimura, Akiko Takashima-Murata, Yoshihiko Shimizu,Akie Takebayahi-Shiraga, Nobuyuki Kita, GuangMei Zhang and Takashi Murakami
  • Journal Title: Tohoku Journal of Experimental medicine Volume: 230
  • NAID: 10031175415
  • Peer Reviewed
• [Presentation] 慢性子宮内膜炎と子宮内膜症の関連性に関する検討 (2013)
  • Author(s): 竹林明枝、木村文則、貴志洋平、杉並洋、清水良彦、高島明子、高橋顕雅、山中章義、喜多伸幸、高橋健太郎、村上節
  • Organizer: 日本産婦人科学会
  • Place of Presentation: 札幌
• [Presentation] The association between endometriosis and chronic endometritis (2013)
  • Author(s): Akie Takebayashi, Fuminori Kimura, Yohei Kishi, Mitsuaki Ishida, , Kentaro Takahashi,, Hiroshi Suginami, and Takashi Murakami
  • Organizer: 日本産婦人科学会
  • Place of Presentation: 日本（札幌）