| Project/Area Number |
24659732
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Ehime University |
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Principal Investigator |
KOIZUMI MASAE 愛媛大学, 医学(系)研究科(研究院), 助教 (60583028)
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| Co-Investigator(Kenkyū-buntansha) |
NAWA Akihiro 愛媛大学, 大学院医学系研究科, 教授 (90242859)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 性分化 / 雌生殖器分化 / Lgr4 |
|---|
| Research Abstract |
Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) is a type of membrane receptor with a seven-transmembrane structure.We observed that Lgr4-/- female mice showed abnormal development of the Wolffian ducts, the appearance of male-like somatic cells in the ovaries. Lgr4-/- female mice exhibited masculinization similar to RSPO1-, beta-catenin-, and Wnt4-deficient mice. In the Lgr4-/- ovarian somatic cells, Lefl and Axin2 expression was reduced, which were target genes of Wnt-beta catenin signaling. On the other hand the androgen receptor (AR) expression in Wolffian ducts and ovarian somatic cells of Lgr4-/- female was increased. This study suggested that Lgr4 was critical to ovarian somatic cell specialization through the cooperative signaling of RSPOs and Wnt and involved the regulation of the AR expression in female reproductive organs.
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