New evaluation of fetal oxidative stress: measurement of the umbilical cord blood dimethyl sulfate-induced ascorbyl free radical by an electron spin resonance method

• Project/Area Number: 24659734
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Oita University
• Principal Investigator: NISHIDA Yoshihiro 大分大学, 医学部, 准教授 (10336274)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 酸化ストレス / 胎児機能不全 / 臍帯 / Vitamin C / ESR / 分娩 / Vitamin C radical / Umbilical cord blood / Fetal stress / 胎児 / 電子スピン共鳴法 / 胎盤 / fetus / oxidative stress / umbilical cord

Outline of Final Research Achievements

Objective. The aim is to evaluate intrapartum fetal oxidative stress in real-time by umbilical cord blood dimethyl sulfate (DMSO)-induced ascorbyl free radical (AFR) measured by an electron spin resonance (ESR) method. Methods. 75 mothers delivering at gestational age after 37 weeks were recruited. Umbilical artery (UA) and venous (UV) cord blood gas analysis was performed. Serum levels of DMSO-induced AFR (AFR/DMSO) that reflect vitamin C concentrations, was measured by ESR spectroscopy. Results. Blood gas analysis showed no significant differences among the groups. UA-AFR/DMSO level of elective cesarean section(CS) group was significantly lower compared with spontaneous delivery group. Emergency CS group showed significantly lower levels of UA-AFR/DMSO compared with elective CS group. UV-AFR/DMSO levels had no significant difference among the groups. Conclusions. It is suggested that fetal cord blood AFR/DMSO is a sensitive marker to assess fetal oxidative stress during delivery.

Research Products

• [Journal Article] Telmisartan induces growth inhibition, DNA double-strand breaks and apoptosis in human endometrial cancer cells. (2014)
  • Author(s): Naoko Koyama, Yoshihiro Nishida, Terukazu Ishii, Toshie Yoshida, Yuichi Furukawa,Hisashi Narahara.
  • Journal Title: PLoS ONE Volume: 9(3)
  • Peer Reviewed / Open Access
• [Journal Article] New evaluation of fetal oxidative stress:measurement of the umbilical cord blood dimethyl sulfate-induced ascorbyl free radical by an electron spin resonance method (2012)
  • Author(s): Yoshihiro Nishida, Shigekiyo Matsumoto, Takashi Noguchi, Hishashi Narahara
  • Journal Title: J Maternal-Fetal Neo Med Volume: 25(1） Issue: 12 Pages: 2499-2502
  • DOI: 10.3109/14767058.2012.704448
  • Peer Reviewed
• [Presentation] ESR によるビタミンC ラジカル値からみた精子機能 (2014)
  • Author(s): 西田欣広、松本重清、原田枝美、山下由貴、古賀寛教、北野敬明、
  • Organizer: 第67回日本酸化ストレス学会
  • Place of Presentation: 同志社大学（京都府京都市）
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] 緊急帝王切開術における妊婦へのビタミンＣ投与が胎児酸化ストレスとApgar score に与える影響 (2014)
  • Author(s): 松本重清、古賀寛教、新宮千尋、小山淑正、内野哲哉、牧野剛典、
  • Organizer: 第67回日本酸化ストレス学会
  • Place of Presentation: 同志社大学(京都府京都市）
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] 胎児酸化ストレスにおけるmalondialdehydeとフリーラジカルとの関連および候補蛋白の抽出 (2012)
  • Author(s): 西田欣広、石井照和、吉良尚子、楢原久司
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Non-reassuring fetal statusにおける酸化ストレスの解析 (2012)
  • Author(s): 西田欣広、楢原久司
  • Organizer: 第12回日本抗加齢医学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Non-reassuring fetal statusと診断された胎児の酸化ストレスマーカーの解析 (2012)
  • Author(s): 西田欣広、石井照和、吉良尚子、楢原久司
  • Organizer: 第48回日本周産期・新生児学会総会
  • Place of Presentation: 大宮ソニックシティ