| Project/Area Number |
24659738
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
ISHIKAWA Gen 日本医科大学, 医学部, 講師 (20287767)
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| Research Collaborator |
ROBINSON John
KAMBE Saori
KURASHINA Ryuhei
MASE Yuri
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 胎盤 / トロホブラスト / クロマチン制御 / 産科学 |
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| Outline of Final Research Achievements |
The function and molecular mechanism of syncytiotrophoblast which shows fused multi-nuclear structure configuring apical layer of feto-maternal blood barrier has not fully been solved.
This study aimed to clarify whether syncytiotrophoblast which shows fused and multi-nuclear unique structure has transcriptional activity or not. And aimed to elucidate some part of transcriptional control of syncytiotrophoblast to develop understanding of mechanism of trophoblast formation and maintaining pregnant state.
As results, morphological findings which suggest chromatin activity were obtained in the terminal villous nuclei after immunohistochemistry. BeWo cells were cultured with forskolin to induce cell fusion. Morphological findings which suggest chromatin activity were obtained in the fused BeWo cells’ nuclei after fluorescent. Additionally, morphological changes were observed under the condition of culture with 5aza-deoxycytidine.
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