New analysis of inner ear pharmacology
| Project/Area Number |
24659752
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Keio University |
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Principal Investigator |
OGAWA Kaoru 慶應義塾大学, 医学部, 教授 (00169179)
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| Co-Investigator(Kenkyū-buntansha) |
KANZAKI Sho 慶應義塾大学, 医学部, 講師 (50286556)
FUJIOKA Masato 慶應義塾大学, 医学部, 共同研究院 (70398626)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 内耳 / 薬物動態 / 鼓室内投与 / 徐放作用 / ドラッグデリバリー |
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| Research Abstract |
Methods 1)Luciferin delivered into the inner ears of GFAP-Luc transgenic mice reacted with luciferase in GFAP-expressing cells in the cochlear spiral ganglion, resulting in photon bioluminescence.Both luciferin and hyaluronic acid (HA) were injected through the left side of ear. We changed the concentration and viscosity of HA. Signals were obtained in the left side of ear in GFAP-luc mice through the IVIS system. 2) Strong intensity noise exposed into male Guinea pigs for two hours. Combination of dexamethasone and HA was administrated into the ear.Results 1) Signal does not depend on the viscosity of hyaluronic acid of inner ear. One reason is that high viscosity of HA can not go through the round window membrane. We considered that the technical problem was involved.2)The question is "Is the combination of steroid and HA successful or not?"ABR was tested before and after treatment.
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Report
(3 results)
Research Products
(10 results)
