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Development of retinal treatment with dental pulp cells

Research Project

Project/Area Number 24659756
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionTohoku University

Principal Investigator

NAKAZAWA Toru  東北大学, 医学(系)研究科(研究院), 教授 (30361075)

Co-Investigator(Kenkyū-buntansha) TANAKA Yuji  東北大学, 大学院医学系研究科, 助教 (40625513)
Co-Investigator(Renkei-kenkyūsha) SASAKI Ryo  東京女子医科大学, 大学院医学研究科, 講師 (60524709)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞治療 / 歯髄 / 網膜障害 / 神経再生 / 歯髄細胞 / 低酸素培養 / 浮遊培養 / 幹細胞 / 網膜 / 神経保護 / 細胞移植 / 網膜神経説細胞 / 再生医療
Outline of Final Research Achievements

The transplantation of dental pulp-derived cells to the eye was explored for the possibility of the novel neuroprotection and regenerative treatments to the critical retinal diseases that results in vision loss. Firstly, the isolated dental pulp-derived cells were directly injected into normal rat vitreous, and it was confirmed that some of the injected cells adhered to the retina and formed the neuronal process. Furthermore, several cell manipulation methods were examined to enrich stem cell population and up-regulate neuron-related molecular expression. Specifically, (1) gene expression of nerve and stem cell related molecule were activates by sphere-forming floating culture, (2) sphere-forming cells can be enriched by cell sorting of CD105-positive dental pulp-derived cells, (3) the expression of an axonal regeneration related molecule was promoted by applying hypoxic culture to the sphere-forming culture.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (31 results)

All 2014 2013 2012 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results) Presentation (15 results) (of which Invited: 7 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells2014

    • Author(s)
      Kunie Hagiwara, Takeshi Obayashi, Nobuyuki Sakayori, Emiko Yamanishi, Ryuhei Hayashi, Noriko Osumi, Toru Nakazawa, Kohji Nishida
    • Journal Title

      PLoS One

      Volume: 9(1) Issue: 1 Pages: 1-13

    • DOI

      10.1371/journal.pone.0084072

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RNA sequence reveals mouse retinal transcriptome changes early after axonal injury2014

    • Author(s)
      Yasuda M, Tanaka Y, Ryu M, Tsuda S, Nakazawa T
    • Journal Title

      PLoS One

      Volume: 9(3) Issue: 3 Pages: e93258-e93258

    • DOI

      10.1371/journal.pone.0093258

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A polymeric device for controlled transscleral multi-drug delivery to the posterior segment of the eye2014

    • Author(s)
      Nobuhiro Nagai, Hirokazu Kaji, Hideyuki Onami, Yumi Ishikawa, Matsuhiko Nishizawa, Noriko Osumi, Toru Nakazawa, Toshiaki Abe
    • Journal Title

      Acta Biomaterialia

      Volume: 10(2) Issue: 2 Pages: 680-6877

    • DOI

      10.1016/j.actbio.2013.11.004

    • NAID

      40020425298

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 緑内障における神経保護治療2014

    • Author(s)
      津田聡、中澤徹
    • Journal Title

      Medical Science Digest

      Volume: 40 (3) Pages: 14-17

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Critical role of Nrf2 in oxidative stress-induced retinal ganglion cell death2013

    • Author(s)
      Himori N, Yamamoto K, Maruyama K, Ryu M, Taguchi K, Yamamoto M, Nakazawa T.
    • Journal Title

      J Neurochem

      Volume: 127 Issue: 5 Pages: 669-680

    • DOI

      10.1111/jnc.12325

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Azo-based Fluorescence Probes to Detect Different Levels of Hypoxia2013

    • Author(s)
      Wen Piao, Satoru Tsuda, Yuji Tanaka, Satoshi Maeda, Fengyi Liu, Shodai Takahashi, Yu Kushida, Toru Komatsu, Tasuku Ueno Takuya Terai, Toru Nakazawa, Masanobu Uchiyama, Keiji Morokuma, Tetsuo Nagano, KenjiroHanaoka
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 52 Issue: 49 Pages: 13028-13032

    • DOI

      10.1002/anie.201305784

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 緑内障に対する神経保護薬の研究開発2013

    • Author(s)
      田中佑治、中澤徹
    • Journal Title

      眼科

      Volume: 55(5) Pages: 601-607

    • NAID

      40019690678

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Molecular Genetic Analysis of Primary Open-angle Glaucoma, Normal Tension Glaucoma, and Developmental Glaucoma for the VAV2 and VAV3 Gene Variants in Japanese Subjects2013

    • Author(s)
      Shi D, Takano Y, Nakazawa T, MengkegaleM, Yokokura S, Nishida K, Fuse N.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 15;432(3) Issue: 3 Pages: 509-512

    • DOI

      10.1016/j.bbrc.2013.02.003

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The regulatory roles of apoptosis-inducing factor in the formation and regression processes of ocular neovascularization.2012

    • Author(s)
      Hisatomi T
    • Journal Title

      Am J Patho

      Volume: 181 Issue: 1 Pages: 53-61

    • DOI

      10.1016/j.ajpath.2012.03.022

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Metabolic stress response implicated in diabetic retinopathy: The role of calpain, and the therapeutic impact of calpain inhibitor.2012

    • Author(s)
      Shanab AY, Nakazawa T, Ryu M, Tanaka Y, Himori N, Taguchi K, Ysuda M, Watanabe R, Takano J, Saido T, Minegishi N, Miyata T, Abe T, Yamamoto M.
    • Journal Title

      Neurobiological Disease

      Volume: 48 Issue: 3 Pages: 556-557

    • DOI

      10.1016/j.nbd.2012.07.025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Critical role of calpain in axonal damage-induced retinal ganglion cell death2012

    • Author(s)
      Molin Ryu, Toru Nakazawa, et al
    • Journal Title

      J Neuroci Res

      Volume: 90 Issue: 4 Pages: 802-815

    • DOI

      10.1002/jnr.22800

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation of CNTNAP2gene polymorphisms for exfoliation syndrome in Japanese2012

    • Author(s)
      Shimizu A, Takano Y, Shi D, Yokokura S,Yokoyama Y, Zheng X, Shiraishi A, Ohashi Y, Nakazawa T, Fuse N.
    • Journal Title

      Mol Vis

      Volume: 18 Issue: 1 Pages: 1395-1401

    • DOI

      10.1371/journal.pone.0054115

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 低酸素応答生理活性蛍光プローブを用いた網膜低酸素領域のin vivo イメージング2013

    • Author(s)
      津田聡、田中佑治、朴文、國方彦志、横山悠、安田正幸、花岡健二郎、長野哲雄、中澤徹
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノ粒子による副腎皮質ステロイド治療薬開発2013

    • Author(s)
      中澤徹
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] エリスロポエチンノックアウトマウスを用いた網膜・視神経の組織学的検討2013

    • Author(s)
      安田正幸、劉孟林、田中佑治、檜森紀子、面高宗子、横山悠、雪田昌克、津田聡、 丸山和一、山本雅之、中澤 徹
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酸化ストレスによる網膜神経節細胞死におけるカルパインの働き2013

    • Author(s)
      横山悠、劉孟林、山本耕太郎、安田正幸、田中佑治、丸山和一、中澤徹
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] RNA-Seqによるマウス視神経挫滅極早期における網膜網羅的遺伝子発現定量解析2013

    • Author(s)
      田中佑治、安田正幸、中澤徹
    • Organizer
      第24回日本緑内障学会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 視神経挫滅モデルにおけるROCK阻害薬K-115の神経保護効果2013

    • Author(s)
      山本耕太郎、丸山和一、檜森紀子、中澤徹
    • Organizer
      第24回日本緑内障学会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 基礎研究を踏まえた夢のある近未来治療2013

    • Author(s)
      中澤徹
    • Organizer
      広島大学・山口大学眼科Grand Rounds
    • Place of Presentation
      山口
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 近未来の緑内障神経保護治療へのアプローチ2013

    • Author(s)
      中澤徹
    • Organizer
      ファガン発売1周年記念講演 in 千葉
    • Place of Presentation
      幕張
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 緑内障、神経保護治療からのアプローチ2013

    • Author(s)
      中澤徹
    • Organizer
      函館眼科医会学術講演会
    • Place of Presentation
      函館
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Advances in basic sciences: implications for clinical management of glaucoma2012

    • Author(s)
      中澤徹
    • Organizer
      The 1st Asia-Pacific Glaucoma Congress (APGC2012)
    • Place of Presentation
      バリ インドネシア
    • Related Report
      2012 Research-status Report
  • [Presentation] The molecular mechanism of glaucomatous optic neuropathy: learning from a mouse model of axonal damage-induced RGC death

    • Author(s)
      中澤徹
    • Organizer
      韓国老化学会(AACL)
    • Place of Presentation
      ソウル 韓国
    • Related Report
      2012 Research-status Report
  • [Presentation] 緑内障における役者たち、細胞レベルの考察

    • Author(s)
      中澤徹
    • Organizer
      Ophthalmic Seminar in 多治見
    • Place of Presentation
      多治見
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 緑内障、神経保護治療からのアプローチ

    • Author(s)
      中澤徹
    • Organizer
      愛媛県眼科フォーラム
    • Place of Presentation
      松山
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 緑内障神経保護治療

    • Author(s)
      中澤徹
    • Organizer
      Tokyo Ophthalmology Club
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 緑内障における役者たち、細胞レベルの考察

    • Author(s)
      中澤徹
    • Organizer
      第16回甲信セミナー
    • Place of Presentation
      甲府
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 眼科 2012年9月臨時増刊号2012

    • Author(s)
      中澤徹
    • Publisher
      神経保護治療の可能性
    • Related Report
      2012 Research-status Report
  • [Remarks] 東北大眼科

    • URL

      http://www.oph.med.tohoku.ac.jp

    • Related Report
      2013 Annual Research Report
  • [Remarks] 東北大学眼科

    • URL

      http://www.oph.med.tohoku.ac.jp

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 視神経障害発祥リスクの判定方法2014

    • Inventor(s)
      田中佑治、安田正幸、面高宗子、中澤徹
    • Industrial Property Rights Holder
      田中佑治、安田正幸、面高宗子、中澤徹
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-017481
    • Filing Date
      2014-01-31
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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