Development of retinal treatment with dental pulp cells
| Project/Area Number |
24659756
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
NAKAZAWA Toru 東北大学, 医学(系)研究科(研究院), 教授 (30361075)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yuji 東北大学, 大学院医学系研究科, 助教 (40625513)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Ryo 東京女子医科大学, 大学院医学研究科, 講師 (60524709)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 細胞治療 / 歯髄 / 網膜障害 / 神経再生 / 歯髄細胞 / 低酸素培養 / 浮遊培養 / 幹細胞 / 網膜 / 神経保護 / 細胞移植 / 網膜神経説細胞 / 再生医療 |
|---|
| Outline of Final Research Achievements |
The transplantation of dental pulp-derived cells to the eye was explored for the possibility of the novel neuroprotection and regenerative treatments to the critical retinal diseases that results in vision loss. Firstly, the isolated dental pulp-derived cells were directly injected into normal rat vitreous, and it was confirmed that some of the injected cells adhered to the retina and formed the neuronal process. Furthermore, several cell manipulation methods were examined to enrich stem cell population and up-regulate neuron-related molecular expression. Specifically, (1) gene expression of nerve and stem cell related molecule were activates by sphere-forming floating culture, (2) sphere-forming cells can be enriched by cell sorting of CD105-positive dental pulp-derived cells, (3) the expression of an axonal regeneration related molecule was promoted by applying hypoxic culture to the sphere-forming culture.
|
Report
(3 results)
Research Products
(31 results)
-
-
-
-
-
-
[Journal Article] Development of Azo-based Fluorescence Probes to Detect Different Levels of Hypoxia2013
Author(s)
Wen Piao, Satoru Tsuda, Yuji Tanaka, Satoshi Maeda, Fengyi Liu, Shodai Takahashi, Yu Kushida, Toru Komatsu, Tasuku Ueno Takuya Terai, Toru Nakazawa, Masanobu Uchiyama, Keiji Morokuma, Tetsuo Nagano, KenjiroHanaoka
-
Journal Title
Angewandte Chemie International Edition
Volume: 52
Issue: 49
Pages: 13028-13032
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Metabolic stress response implicated in diabetic retinopathy: The role of calpain, and the therapeutic impact of calpain inhibitor.2012
Author(s)
Shanab AY, Nakazawa T, Ryu M, Tanaka Y, Himori N, Taguchi K, Ysuda M, Watanabe R, Takano J, Saido T, Minegishi N, Miyata T, Abe T, Yamamoto M.
-
Journal Title
Neurobiological Disease
Volume: 48
Issue: 3
Pages: 556-557
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
