Autologous transplantation of conjunctival epithelial stem cells based on epigenetical control.

• Project/Area Number: 24659757
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: MIMURA Tatsuya 東京女子医科大学, 医学部, 講師 (70463867)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 結膜再生 / エピジェネティクス / 結膜 / エピジェネティク / 幹細胞

Outline of Final Research Achievements

We developed an adult precursor/stem cell isolation method (the sphere-forming assay) for conjunctival epithelial cells from donor eye and rabbit eye. We demonstrated conjunctival precursor/stem cells possessed the higher proliferative capacity as indicated by BrDU staining and the immature state as indicated by nestin staining compared with cultured differentiated epithelial cells. We cultured conjunctival precursor/stem cells on the amniotic membrane and demonstrated that cultured conjuncdtival epithelium formed three to four layers on amniotic membrane. After transplantation of reconstructed conjunctival sheet on the rabbit conjunctiva and cornea with corneal epithelial opacity, there was no inflammation in the conjunctiva and cornea maintained corneal clarity. These results suggest that transplantation of reconstructed conjunctival epithelial sheet using cultured precursor/stem cells is a possible treatment strategy for treating total limbal stem cell deficiency.

Research Products

• [Journal Article] Retinal neuroprotective effect of Sirtuins. (2014)
  • Author(s): Mimura T, Noma H, Funatsu H, Kondo A, Matsubara M.
  • Journal Title: SM Ophthalmol Volume: 2 Pages: 1-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Next-generation sequencers: What can we learn? (2013)
  • Author(s): Mimura T, Noma H, Funatsu H, Yamagami S.
  • Journal Title: J Bioanal Biomed Volume: 5 Issue: 2 Pages: 1-3
  • DOI: 10.4172/1948-593x.1000e113
  • URL: https://pure.teikyo.jp/en/publications/ab71fafd-ebc7-442c-bad8-d20a1c9077e6
  • Peer Reviewed
• [Journal Article] The role of SIRT1 in ocular aging (2013)
  • Author(s): Mimura T, Kaji Y, Noma H, Funatsu H, Okamoto S.
  • Journal Title: Exp Eye Res Volume: 116 Pages: 17
  • DOI: 10.1016/j.exer.2013.07.017
  • URL: https://localhost/en/publications/9b2c743a-2178-4739-95ed-a4bb812dd4bc
  • Peer Reviewed
• [Journal Article] Corneal endothelial regeneration and tissue engineering (2013)
  • Author(s): Mimura T, Yamagami S, Amano S.
  • Journal Title: Prog Retin Eye Res Volume: 35 Pages: 1
  • DOI: 10.1016/j.preteyeres.2013.01.003
  • URL: https://pure.teikyo.jp/en/publications/5a136e88-50db-4a05-840a-7dbdacf76a13
  • Peer Reviewed
• [Journal Article] Vitreous inflammatory factors and macular oedema (2012)
  • Author(s): Funatsu H, Noma H, Mimura T, Eguchi S
  • Journal Title: Br J Ophthalmol Volume: 96 Issue: 2 Pages: 302
  • DOI: 10.1136/bjo.2010.181222
  • URL: https://localhost/en/publications/a5f6b3bd-dc2e-4207-8879-983792d85195
  • Peer Reviewed
• [Journal Article] Pinguecula and diabetes mellitus (2012)
  • Author(s): Mimura T, Obata H, Usui T, Mori M, Yamagami S, Funatsu H, Noma H, Amano S
  • Journal Title: Cornea Volume: 31 Issue: 3 Pages: 264
  • DOI: 10.1097/ico.0b013e3182254170
  • URL: https://pure.teikyo.jp/en/publications/1432058f-036d-4aba-aea5-c6c1c15eba7a
  • Peer Reviewed
• [Journal Article] Tissue engineering of corneal endothelium. (2012)
  • Author(s): Mimura T, Yokoo S, Yamagami S.
  • Journal Title: J. Funct. Biomater. Volume: 3 Issue: 4 Pages: 726-744
  • DOI: 10.3390/jfb3040726
  • Peer Reviewed
• [Journal Article] Conjunctivochalasis: class FormattedString { value: Associations with pinguecula in a hospital-based study } (2011)
  • Author(s): Mimura T, Mori M, Obata H, Usui T, Yamagami S, Funatsu H, Noma H, Amano S
  • Journal Title: Acta Ophthalmol Volume: 90 Issue: 8 Pages: 773
  • DOI: 10.1111/j.1755-3768.2011.02161.x
  • URL: https://localhost/en/publications/ef69e8c7-fac2-4dc3-8cb2-bc009989bb4b
  • Peer Reviewed
• [Presentation] 角膜内皮の老化と再生 (2013)
  • Author(s): 三村達哉
  • Organizer: 第80回東京女子医大 東医療センター 眼科医局講演会 特別講演
  • Place of Presentation: 東京女子医科大学東医療センター
  • Invited
• [Presentation] イムノキャップラピッドを用いた涙液中特異的IgEと皮膚プリックテストの比較
  • Author(s): 三村達哉、山上聡、臼井智彦、宮井尊史、天野史
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] アレルギー性結膜炎患者涙液中の小麦特異的IgE
  • Author(s): 三村達哉、亀井裕子、五嶋摩理、近藤亜紀、松原
  • Organizer: 第66回日本臨床眼科学会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都
• [Presentation] 細隙灯顕微鏡所見を正確にとるための解剖学・生理学
  • Author(s): 三村達哉
  • Organizer: 角結膜疾患診療ナビ 学術講演会
  • Place of Presentation: 東京ステーションホテル
  • Invited
• [Presentation] ヒト角膜内皮細胞の全ゲノム解析によるDNAメチル化プロファイル
  • Author(s): 三村達哉、中川卓、山上聡、天野史郎
  • Organizer: 角膜カンファランス2013
  • Place of Presentation: 和歌山県 南紀白浜
• [Presentation] Specific Tear IgE in Seasonal Allergic Conjunctivitis with ImmunoCAP Rapid
  • Author(s): Tatsuya Mimura, Satoru Yamagami, Tomohiko
  • Organizer: The Assosiation for Research in Vision and Ophthalmology
  • Place of Presentation: Greater Fort Lauderdale/Broward County Convention Center