Analysis of the Cellular Dynamics of Immune Cells in a Corneal Wound Mouse Model using Intravital Imaging
| Project/Area Number |
24659766
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MIZUNO RENTARO 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10516008)
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| Co-Investigator(Kenkyū-buntansha) |
UETA Mayumi 同志社大学, 生命医科学部, 准教授 (60398386)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 眼免疫学 / 生体イメージング / 生体４Ｄイメージング / 蛍光標識マウス / 角膜 |
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| Research Abstract |
Corneal suture is a surgical procedure used to treat corneal trauma or for cases of corneal transplantation. However, suture-related inflammatory cell infiltration (dynamics) in the cornea has yet to be analyzed in detail. In this study, we used immune cells labeled mice, which are gene-targeted mice expressing enhanced green fluorescent protein (EGFP), and intravital imaging, which is a technique used to analyze cellular and molecular dynamics stereoscopically and time-dependently in an in vivo animal model. LysM strongly positive cells (neutrophils) are present in the stroma of only limbus at normal condition, but they move to the entire stroma of the cornea after alkali injury. We also found that neutrophils migrated from limbal capillary loop and infiltrated to the corneal suture. Using Intravital Imaging, we could find the dynamic features underlying various physiological and pathological conditions in cornea.
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Report
(3 results)
Research Products
(21 results)
