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Atopic keratoconjunctivitis and natural helper cell

Research Project

Project/Area Number 24659768
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionJuntendo University

Principal Investigator

MURAKAMI Akira  順天堂大学, 医学部, 教授 (90157743)

Co-Investigator(Kenkyū-buntansha) EBIHARA Nobuyuki  順天堂大学, 医学部, 教授 (20255699)
MATSUDA Akira  順天堂大学, 医学部, 准教授 (00312348)
Co-Investigator(Renkei-kenkyūsha) NAKAE Susumu  東京大学, 医科学研究所, 准教授 (60450409)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsアトピー性角結膜炎 / ナチュラルヘルパー細胞 / IL-33 / 自然免疫 / パパイン / 春期カタル / アトピー / 結膜炎
Research Abstract

We made innate immune system-dependent mouse conjunctivitis model using papain-absorbed contact lens. The lens was inserted into the conjunctival sac of B6 wild and B6 Rag2-kockout (KO) and IL-33 KO mice. In this mouse models, prominent eosinophilic infiltration was observed in the conjunctival tissue. The number of infiltrated eosinophils was significantly smaller in IL-33 KO mice compared to wild-type and Rag2-KO mice. These results suggested that IL-33 expression is essential for ocular surface allergic inflammatory process through the activation of innate immune systems. In addition, we detected natural helper (NH) cells in the lacrimal gland and conjunctival tissue of the innate immune system-dependent mouse conjunctivitis model. Moreover, we identified NH cell in the giant papillae tissue obtained from severe chronic allergic conjunctivitis patients. These results suggested that NH cell may participate the pathophysiological process of severe chronic allergic conjunctivitis.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (6 results)

  • [Journal Article] Vernal keratoconjunctivitis with giant papillae on the inferior tarsal conjunctiva2014

    • Author(s)
      Asada Y, Ebihara N, Funaki T, Norihiko Y, Murakami A, Matsuda A
    • Journal Title

      Cornea

      Volume: 33 Pages: 32-34

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between conjunctival epithelial cells and mast cells induces CCL2 expression and piecemeal degranulation in mast cells2013

    • Author(s)
      Iwamoto S, Asada Y, Ebihara N, Hori K, Okayama Y, Kashiwakura JI, Watanabe Y, Kawasaki S, Yokoi N, Inatomi T, Shinomiya K, Murakami A, Matsuda A
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 54 Pages: 2465-2473

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between conjunctival epithelial cells and mast cells induces CCL2 expression and piecemeal degranulation in mast cells.2013

    • Author(s)
      Iwamoto S, Asada Y, Ebihara N, Hori K, Okayama Y, Kashiwakura JI, Watanabe Y, Kawasaki S, Yokoi N, Inatomi T, Shinomiya K, Murakami A, Matsuda A.
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 54 Pages: 2465-73

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] IL-33-mediated innate response and adaptive immune cells contribute to maximum responses of protease allergen-induced allergic airway inflammation.2013

    • Author(s)
      Kamijo S, Takeda H, Tokura T, Suzuki1 M, Inui K, Hara M, Matsuda H, Matsuda A, Oboki K, Ohno T, Saito H, Nakae S, Sudo K, Suto H, Ichikawa S, Ogawa H, Okumura K, and Takai T.
    • Journal Title

      J Immunol.

      Volume: In press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Interleukin(IL)-33 and Thymic Stromal Lymphopoietin (TSLP), but not IL-25, induced Allergic Conjunctivitis in Mouse Models Papain-Induced Conjunctivitis2014

    • Author(s)
      Yosuke Asada, Akira Matsuda, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Akira Murakami, Susumu Nakae
    • Organizer
      The annual meeting for the Association of Research in Vision and Ophthalmology
    • Place of Presentation
      米国オーランド
    • Related Report
      2013 Final Research Report
  • [Presentation] Interleukin(IL)-33 and Thymic Stromal Lymphopoietin (TSLP), but not IL-25, induced Allergic Conjunctivitis in Mouse Models Papain-Induced Conjunctivitis2014

    • Author(s)
      Yosuke Asada, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Susumu Nakae, Akira Murakami, Akira Matsuda
    • Organizer
      Annual meeting for the association of research for vision and ophthalmology
    • Place of Presentation
      米国フロリダ州オーランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] The role of interleukin-33 (IL-33) in mouse experimental allergic conjunctivitis2013

    • Author(s)
      Akira Matsuda, Kanji Hori, Waka Ishida, Ken Fukuda, Nobuyuki Ebihara, Yousuke Asada, Susumu Nakae, Atsuki Fukushima, Akira Murakami. Keystone Symposia
    • Organizer
      Type 2 Immunity : Initiation , maintainance, homeostasis and Pathology
    • Place of Presentation
      米国サンタフェ
    • Related Report
      2013 Final Research Report
  • [Presentation] 中江進パパイン誘発結膜炎モデルマウスとナチュラルヘルパー細胞2013

    • Author(s)
      浅田洋輔、松田彰、堀寛爾、岩本怜、海老原伸行、村上晶
    • Organizer
      日本眼科学会総会
    • Related Report
      2013 Final Research Report
  • [Presentation] Detection of natural helper cells in innate immune system-dependent mouse conjunctivitis model2013

    • Author(s)
      Yosuke Asada, Akira Matsuda, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Akira Murakami, Susumu Nakae
    • Organizer
      The annual meeting for the Association of Research in Vision and Ophthalmology
    • Place of Presentation
      米国シアトル
    • Related Report
      2013 Final Research Report
  • [Presentation] パパイン誘発結膜炎モデルマウスとナチュラルヘルパー細胞2013

    • Author(s)
      浅田洋輔、松田 彰 他
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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