| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
Adipose stem cells have been reported to accelerate alveolar and vascular regeneration in damaged rat lungs. Therefore, we are investigating the possibility of regeneration therapy using human umbilical cord mesenchymal stem cells in an experimental hypoplastic lung models.
We used stem cells (hMSC-UC) which are commercially available from PromoCell. １．I used P2 passage culture cells. I created hMSC-UC sheet in 1cm diameter for 5 days culture.２．The ratio of the terminal bud increments of cultured lung was calculated. VEGF, BMP-4, TTF-1 mRNA expression levels were analyzed by RT-qPCR. Terminal lung buds significantly proliferated in embryonic lungs when co-cultured with hMSC-UC. VEGF, BMP-4, and TTF-1 mRNA expressions were all significantly higher in normal embryonic lungs when co-cultured with hMSC-UC. The study showed that hMSC-UC may stimulate secretion of lung growth factors such as; VEGF, BMP-4, and TTF-1 from the embryonic lung.
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