Development of the rapid characterization method of the bacteria in a wound by DNA analysis
| Project/Area Number |
24659779
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
TACHI Masahiro 東北大学, 医学(系)研究科(研究院), 教授 (50312004)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 外科 / 微生物 / 遺伝子 / 創傷治癒学 |
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| Research Abstract |
The culture results of samples from soft tissues infections are often incorrect, and the identification of bacteria takes few days. To develop the rapid and correct bacterial characterization, 16S rRNA analysis were performed. We extracted bacterial DNA raidly and identified bacteial 16S rRNA. To get more rapidly reports from central clinical laboratory, organization of laboratory system will be needed.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Neutrophil-derived tumor necrosis factor-α contributes to acute wound healing promoted by N-(3-oxododecanoyl)-l-homoserine lactone from Pseudomonas aeruginosa.2013
Author(s)
Kanno, E., Kawakami, K, Miyairi, S., Tanno, H., Otomaru, H., Hatanaka, A., Sato, S., Ishii, K., Hayashi, D., Shibuya, N., Imai, Y., Gotoh, N., Maruyama, R., and Tachi, M.
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Journal Title
J. Dermatol. Sci.
Volume: 70
Issue: 2
Pages: 130-138
DOI
Related Report
Peer Reviewed
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[Journal Article] Predictive validity of weekly monitoring of wound status using DESIGN-R score change for pressure ulcer healing : a multicenter prospective cohort study2012
Author(s)
Iizaka S, Sanada H, Matsui Y, Furue M, Tachibana T, Nakayama T, Sugama J, Furuta K, Tachi M, Tokunaga K, Miyachi Y
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Journal Title
Wound Repair Regen
Volume: 20
Issue: 4
Pages: 473-481
DOI
NAID
Related Report
Peer Reviewed
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