Development of the rapid characterization method of the bacteria in a wound by DNA analysis

• Project/Area Number: 24659779
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Tohoku University
• Principal Investigator: TACHI Masahiro 東北大学, 医学(系)研究科(研究院), 教授 (50312004)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 外科 / 微生物 / 遺伝子 / 創傷治癒学

Research Abstract

The culture results of samples from soft tissues infections are often incorrect, and the identification of bacteria takes few days. To develop the rapid and correct bacterial characterization, 16S rRNA analysis were performed. We extracted bacterial DNA raidly and identified bacteial 16S rRNA. To get more rapidly reports from central clinical laboratory, organization of laboratory system will be needed.

Research Products

• [Journal Article] Neutrophil-derived tumor necrosis factor-α contributes to acute wound healing promoted by N-(3-oxododecanoyl)-l-homoserine lactone from Pseudomonas aeruginosa. (2013)
  • Author(s): Kanno, E., Kawakami, K, Miyairi, S., Tanno, H., Otomaru, H., Hatanaka, A., Sato, S., Ishii, K., Hayashi, D., Shibuya, N., Imai, Y., Gotoh, N., Maruyama, R., and Tachi, M.
  • Journal Title: J. Dermatol. Sci. Volume: 70 Issue: 2 Pages: 130-138
  • DOI: 10.1016/j.jdermsci.2013.01.004
  • Peer Reviewed
• [Journal Article] Predictive validity of weekly monitoring of wound status using DESIGN-R score change for pressure ulcer healing : a multicenter prospective cohort study (2012)
  • Author(s): Iizaka S, Sanada H, Matsui Y, Furue M, Tachibana T, Nakayama T, Sugama J, Furuta K, Tachi M, Tokunaga K, Miyachi Y
  • Journal Title: Wound Repair Regen Volume: 20 Issue: 4 Pages: 473-481
  • DOI: 10.1111/j.1524-475x.2012.00778.x
  • NAID: 10031181834
  • Peer Reviewed
• [Journal Article] Low-energy extracorporeal shock wave therapy enhances skin wound healing in diabetic mice : A critical role of endotherial nitric oxide synthase (2012)
  • Author(s): Hayashi D, Kawakami K, Ito K, Ishii K, Tanno H, Kanno E, Maruyama R, Shimokawa H, Tachi M
  • Journal Title: Wound Repair Regen Volume: 20 Issue: 6 Pages: 887-895
  • DOI: 10.1111/j.1524-475x.2012.00851.x
  • Peer Reviewed
• [Presentation] 緑膿菌クオラムセンシング分子N-butanoyl-L-homoserine lactoneが創傷治癒過程と創部炎症反応に与える影響 (2013)
  • Author(s): 鈴木愛子, 菅野恵美, 川上和義, 宮入伸一, 丹野寛大, 石井恵子, 丸山良子, 館正弘
  • Organizer: 第24回日本生体防御学会
  • Place of Presentation: 熊本
• [Presentation] 緑膿菌クオラムセンシング分子N-butanoyl-L-homoserine lactoneが創傷治癒過程と創部炎症反応に与える影響 (2012)
  • Author(s): 菅野恵美, 丹野寛大, 川上和義, 宮入伸一, 石井恵子, 丸山良子, 館正弘
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 札幌
• [Presentation] Skin wound healing promoted by a quorum-sensing moleclue, N-(3-Oxododecanoyl)-L-homoserine lactone from Pseudomonas aeruginosa (2012)
  • Author(s): Kanno E, Kawakami K, Miyairi S, TannoH, Ishii K, Hayashi D, Maruyam R, Tachi M
  • Organizer: 4th Congress of the World Union of Wound Healing Societie
  • Place of Presentation: 横浜
• [Presentation] Role of invariant natural killer T cells in skin wound healing (2012)
  • Author(s): Tanno H, Kawakami K, Kanno E, Hayashi D, Ishii K, Maruyama R, Tachi M
  • Organizer: 4th Congress of the World Union of Wound Healing Societie
  • Place of Presentation: 横浜
• [Presentation] Skin wound healing promoted by a quorum-sensing moleclue, N-(3-Oxododecanoyl)-L-homoserine lactone from Pseudomonas aeruginosa. (2012)
  • Author(s): Kanno E, Kawakami K, Miyairi S, Tanno H, Ishii K, Hayashi D, Maruyama R, Tachi M
  • Organizer: WUWHS
  • Place of Presentation: Yokohama
• [Presentation] Role of invariant natural killer T cells in the skin wound healing. (2012)
  • Author(s): Tanno H, Kawakami K, Kanno E, Hayashi D, Ishii K, Maruyama R, Tachi M
  • Organizer: WUWHS
  • Place of Presentation: Yokohama
• [Presentation] 緑膿菌クオラムセンシング分子N-butanoyl-L-homoserine lactoneが創傷治癒過程に与える影響 (2012)
  • Author(s): 菅野恵美, 丹野寛大, 川上和義, 宮入伸一, 石井恵子, 丸山良子, 館正弘
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 札幌
• [Book] 創傷のすべて第V章感染症2.慢性感染症Critical colonization (2012)
  • Author(s): 菅野恵美, 館正弘
  • Publisher: 克誠堂出版
• [Book] ナースのためのアドバンスド創傷ケアPartIIアドバンスド創傷ケアの実際2.実際編創傷病態生理学:各論慢性創傷の病態 (2012)
  • Author(s): 菅野恵美, 館正弘
  • Publisher: 学照林社
• [Book] 創傷のすべて 第V章 感染症 2. 慢性感染症 Critical colonization (2012)
  • Author(s): 菅野恵美, 館正弘
  • Publisher: 克誠堂出版
• [Book] ナースのためのアドバンスド創傷ケアPartIIアドバンスド創傷ケアの実際 2. 実際編 創傷病態生理学: 各論 慢性創傷の病態学. (2012)
  • Author(s): 菅野恵美, 館正弘
  • Publisher: 照林社
• [Remarks]
  • URL: http://www.prs.med.tohoku.ac.jp/
• [Remarks] 東北大学形成外科HP
  • URL: http://www.prs.med.tohoku.ac.jp/index.html