| Project/Area Number |
24659782
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Katsuya 京都大学, 医学研究科, 准教授 (90273458)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAOKA Tetsuji 国立循環器病研究センター研究所, 生体医工学部, 部長 (50243126)
FUJISATO Toshia 大阪工業大学, 工学部, 教授 (60270732)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 再生医学 / 脱細胞化 / 皮膚 / 母斑 / 細胞・組織 / 臨床 |
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| Research Abstract |
In this study, we prepared the acellular dermal matrix from the skin of giant melanocytic nevi using hypertonic saline (1 N NaCl), 0.05 % trypsin, 0.1 % SDS (sodium dodecyl sulfate),
and high-hydrostatic pressurization (HHP). No obvious damage to dermis was observed in all methods. SDS could decellularize skin easily, however it had cytotoxicity to prevent the attachment of cells. HHP was also useful for decellularization and it could be applied in a clinical application.
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