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Differentiated adipose-derived stem cells promote reinnervation of rat skin flaps

Research Project

Project/Area Number 24659783
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plastic surgery
Research InstitutionOsaka University

Principal Investigator

TOMITA Koichi  大阪大学, 医学(系)研究科(研究院), 助教 (90423178)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords移植皮弁知覚 / 脂肪組織由来間葉系幹細胞 / シュワン細胞 / 再生医学 / 皮弁知覚
Research Abstract

The aim of this study was to elucidate if Schwann cell (SC)-like cells differentiated from adipose-derived stem cells (ASCs) could improve the cutaneous nerve regeneration in skin flaps. Island groin flaps were elevated bilaterally in 32 rats. ASCs, SC-like cells differentiated from ASCs (dASCs), or SCs were injected to the flaps, and the reinnervation density was immunohistochemically assessed.
Although a long-term survival of the transplanted cells was not found, dASCs and SCs significantly increased reinnervation density in the periphery of flaps, and this was more pronounced in noninnervated flaps. On the other hand, ASC showed no statistically significant effect on the peripheral reinnervation. In the center of flap, there was no statistically significant increase in reinnervation density in all groups irrespective of flap innervation.
In addition to the use of innervated flaps, dASC transplantation therapy could be a new approach to improve the sensory recovery of skin flaps.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (9 results)

  • [Journal Article] Differentiated adipose-derived stem cells promote reinnervation of rat skin flaps2013

    • Author(s)
      Tomita K, Nishibayashi A, Yano K, Hosokawa K
    • Journal Title

      Plastic and Reconstructive Surgery Global Open

      Volume: 1(3) Pages: 22-22

    • Related Report
      2013 Final Research Report
  • [Journal Article] Glial differentiation of human adipose-derived stem cells : Implications for cell-based transplantation therapy2013

    • Author(s)
      Tomita K, Madura T, Sakai Y, Yano K, Terenghi G, Hosokawa K
    • Journal Title

      Neuroscience

      Volume: 236(16) Pages: 55-65

    • Related Report
      2013 Final Research Report
  • [Journal Article] Differentiated adipose-derived stem cells promote reinnervation of rat skin flaps.2013

    • Author(s)
      Tomita K, Nishibayashi A, Yano K, Hosokawa K
    • Journal Title

      Plastic and Reconstructive Surgery Global Open

      Volume: 1(3) Issue: 3 Pages: e22-e22

    • DOI

      10.1097/gox.0b013e318299134d

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glial differentiation of human adipose-derived stem cells: Implications for cell-based transplantation therapy.2013

    • Author(s)
      Tomita K, Madura T, Sakai Y, Yano K, Terenghi G, Hosokawa K
    • Journal Title

      Neuroscience

      Volume: 236(16) Pages: 55-65

    • DOI

      10.1016/j.neuroscience.2012.12.066

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Differentiated adipose-derived stem cells promote cutaneous nerve regeneration in rat skin flaps2013

    • Author(s)
      Tomita K, Hosokawa K
    • Organizer
      5^<th> European Plastic Surgery Research Council
    • Place of Presentation
      Hamburg, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] 脂肪組織由来間葉系幹細胞移植による皮弁知覚向上の試み-移植皮弁における正常知覚獲得を目指して-2013

    • Author(s)
      冨田興一、西林章光、矢野健二、細川亙
    • Organizer
      日本マイクロサージャリー学会40周年記念学術集会
    • Place of Presentation
      岩手県盛岡市
    • Related Report
      2013 Final Research Report
  • [Presentation] 移植皮弁における正常知覚獲得を目指して-脂肪組織由来間葉系幹細胞移植による皮弁知覚向上の試み-2013

    • Author(s)
      冨田興一、西林章光、矢野健二、細川亙
    • Organizer
      第22回日本形成外科学会学術集会
    • Place of Presentation
      新潟県新潟市
    • Related Report
      2013 Final Research Report
  • [Presentation] Differentiated adipose-derived stem cells promote cutaneous nerve regeneration in rat skin flaps2013

    • Author(s)
      Tomita K, Hosokawa K
    • Organizer
      5th European Plastic Surgery Research Council
    • Place of Presentation
      Hamburg, Germany
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脂肪組織由来間葉系幹細胞移植による皮弁知覚向上の試み -移植皮弁における正常知覚獲得を目指して-2013

    • Author(s)
      冨田興一、西林章光、矢野健二、細川亙
    • Organizer
      日本マイクロサージャリー学会40周年記念学術集会 シンポジウム2 「知覚皮弁の適応と成績」
    • Place of Presentation
      岩手県盛岡市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 移植皮弁における正常知覚獲得を目指して ‐脂肪組織由来間葉系幹細胞移植による皮弁知覚向上の試み-2013

    • Author(s)
      冨田興一、西林章光、矢野健二、細川亙
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      新潟県新潟市
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト脂肪組織由来間葉系幹細胞による神経内細胞治療法の開発2013

    • Author(s)
      冨田興一 Giorgio Terenghi 細川亙
    • Organizer
      第12回 日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒト脂肪組織由来間葉系幹細胞による神経内細胞治療法の開発‐標的組織の早期神経再支配を目指して‐2012

    • Author(s)
      冨田興一 他
    • Organizer
      第21回 日本形成外科学会基礎学術集会シンポジウム3 「Long march ~組織再生迅速化の試み」
    • Place of Presentation
      福島
    • Related Report
      2012 Research-status Report
  • [Presentation] Differentiated Adipose-derived Stem Cells Promote Myelination and Enhance Functional Recovery in a Rat Model of Chronic Denervation2012

    • Author(s)
      Koichi Tomita, Tomas Madura, Giorgio Terenghi, Ko Hosokawa
    • Organizer
      57th Plastic Surgery Research Council
    • Place of Presentation
      Ann Arbor, Michigan, USA
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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