Efficient cartilage generation from human progenitors by recapitulating transient vascularization

• Project/Area Number: 24659785
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Yokohama City University
• Principal Investigator: TAKEBE Takanori 横浜市立大学, 医学部, 准教授 (20612625)
• Co-Investigator(Kenkyū-buntansha): TANIGUCHI Hideki 横浜市立大学, 医学研究科, 教授 (70292555) ; KOBAYASHI Shinji 横浜市立大学, 医学研究科, 客員准教授 (90464536)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 移植・再生医療 / 再生医学

Research Abstract

Here, we report that avascular elastic cartilage could be efficiently regenerated from in vitro grown mesenchymal condensation by recapitulating early chondrogenesis. Combined with an intravital imaging approach, we identified the presence of endothelial cells inside the mesenchymal condensation prior to cartilage maturation. Strikingly, recreating endothelial interaction in culture enabled the recently identified adult progenitors to generate a mesenchymal condensation in vitro in a self-driven manner without any support of exogenous factors nor scaffolds. These in vitro grown 3-D condensed progenitors much efficiently reconstructed three-dimensional elastic cartilage upon transplantation compared with conventional methods. Thus, the transplantation of condensed progenitors with endothelial cells represents a promising new approach towards realizing regenerative medicine as well as studying the mechanism of vascular regression.

Research Products

• [Journal Article] Reconstruction of Joint Hyaline Cartilage by Autologous Progenitor Cell s Derived from Ear Elastic Cartilage (2014)
  • Author(s): M. Mizuno, S. Kobayashi, T. Takebe, H. Kan, Y. Yabuki, T. Matsuzaki, H. Y. Yoshikawa, S. Nakabayashi, L. J. Ik, J. Maegawa, H. Taniguchi
  • Journal Title: Stem Cells Volume: 32 Issue: 3 Pages: 816-821
  • DOI: 10.1002/stem.1529
  • Peer Reviewed / Open Access
• [Journal Article] Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): Takanori Takebe*, Ran-Ran Zhang, Hiroyuki Koike, Masaki Kimura, Emi Yoshizawa, Masahiro Enomura, Keisuke Sekine, Hideki Taniguchi*(*: Correspondence)
  • Journal Title: Nature Protocols Volume: 9(2) Issue: 2 Pages: 396-409
  • DOI: 10.1038/nprot.2014.020
  • Peer Reviewed
• [Journal Article] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe*, Keisuke Sekine, Masahiro Enomura, Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Yun-Wen Zheng, Naoto Koike, Shinsuke Aoyama, Yasuhisa Adachi, Hideki Taniguch*(*: Correspondence)
  • Journal Title: Nature Volume: 499 Issue: 7459 Pages: 481-484
  • DOI: 10.1038/nature12271
  • NAID: 40020351046
  • Peer Reviewed
• [Journal Article] The generation of pancreatic β-cell spheroids in a simulated microgravity culture system. (2013)
  • Author(s): Tanaka H
  • Journal Title: Biomaterials Volume: 34 Issue: 23 Pages: 5785-5791
  • DOI: 10.1016/j.biomaterials.2013.04.003
  • Peer Reviewed
• [Journal Article] Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor. (2012)
  • Author(s): Takanori Takebe, Shinji Kobayashi, Hiroomi Kan, Hiromu Suzuki, Mitsuru Mizuno, Yuichiro Yabuki, Takuro Adegawa, Tomohiko Yoshioka , Junzo Tanaka, Jiro Maegawa, Hideki Taniguchi.
  • Journal Title: Transplantation Proceedings Volume: 44 Issue: 4 Pages: 1158-61
  • DOI: 10.1016/j.transproceed.2012.03.038
  • Peer Reviewed
• [Journal Article] Self-organization of human hepatic organoid by recapitulating organogenesis in vitro (2012)
  • Author(s): Takebe T
  • Journal Title: Transplant Pro Volume: 44(4) Issue: 4 Pages: 1018-1020
  • DOI: 10.1016/j.transproceed.2012.02.007
  • Peer Reviewed
• [Journal Article] Generation of functional human vascular network in vitro (2012)
  • Author(s): Takebe T
  • Journal Title: Transplant Proc Volume: 44(4) Issue: 4 Pages: 1130-1133
  • DOI: 10.1016/j.transproceed.2012.03.039
  • Peer Reviewed
• [Journal Article] Highly efficient generation of definitive endoderm lineage from human induced pluripotent stem cells (2012)
  • Author(s): Sekine K
  • Journal Title: Transplant Proc Volume: 44(4) Issue: 4 Pages: 1127-1129
  • DOI: 10.1016/j.transproceed.2012.03.001
  • Peer Reviewed
• [Journal Article] Regenerative medicine approach as an alternative treatment to islet transplantation. (2012)
  • Author(s): Sekine K
  • Journal Title: Transplant Proc Volume: 44(4) Issue: 4 Pages: 1104-1106
  • DOI: 10.1016/j.transproceed.2012.03.020
  • Peer Reviewed
• [Journal Article] Establishment of Automated Culture System for Murine Induced Pluripotent Stem Cells (2012)
  • Author(s): Koike H
  • Journal Title: BMC Biotechnology Volume: 12 Issue: 1 Pages: 81-81
  • DOI: 10.1186/1472-6750-12-81
  • Peer Reviewed
• [Journal Article] 細胞から臓器へ、再生医療研究が拓く近未来像 (2012)
  • Author(s): 武部貴則
  • Journal Title: Organ Biology Volume: 19(1)
  • NAID: 130004710042
  • Peer Reviewed
• [Presentation] 臓器原基創出法を活用した多細胞系からなる複雑な立体組織の人為的構成 (2014)
  • Author(s): 武部貴則、吉川洋史、谷口英樹
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 国立京都国際会館(京都府)シンポジウム
  • Invited
• [Presentation] Realization of human iPSC-derived organ bud transplantation therapy. (2014)
  • Author(s): Takanori Takebe
  • Organizer: The Whole Liver Replacement State-of-the-Science Summit
  • Place of Presentation: Chicago, USA
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): Takanori Takebe
  • Organizer: 7th Pan Pacific Symposium on Stem Cells and Cancer Research (PPSSC)
  • Place of Presentation: the Splendor Hotel of Taichung, Taiwan
  • Invited
• [Presentation] 臓器原基創出法を活用した多細胞系からなる複雑な立体組織の人為的構成 (2014)
  • Author(s): 武部貴則、吉川洋史、谷口英樹
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 国立京都国際会館（京都府）
  • Invited
• [Presentation] ヒトiPS細胞由来臓器原基移植による機能的なヒト肝臓の創出 (2014)
  • Author(s): 武部貴則、関根圭輔、谷口英樹
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 国立京都国際会館（京都府）
  • Invited
• [Presentation] Phenotypic comparison of canine articular and auricular derived chondrocytes for cartilage reconstruction (2013)
  • Author(s): Mizuno M, Takebe T, Kobayashi S, Lee S, Jo YH, Lee JI, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kyoto, Japan
• [Presentation] Transient vascularization directs intrinsic cartilage formation from human progenitors (2013)
  • Author(s): Takebe T, Kobayashi S, Mizuno M, Suzuki H, Chang Yu-Min, Yoshizawa E, Maegawa J, Taniguchi H
  • Organizer: International Society for Stem Cell Research 11^<th> annual meeting
  • Place of Presentation: Boston, USA(Poster)
• [Presentation] ヒト耳介由来軟骨前駆細胞を用いた弾性軟骨再構築法の開発 (2013)
  • Author(s): 武部貴則、小林眞司、谷口英樹
  • Organizer: 第12回日本再生医療学会シンポジウム「組織幹細胞の最前線」
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Creation of vascularized and functional human liver from an induced pluripotent stem cell-derived organ bud transplant. (2013)
  • Author(s): Takebe T, Sekine K, Enomura M, Suzuki Y, Koike H, Zhang R, Koike N, Ueno Y, Zheng Y, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Phenotypic comparison of canine articular and auricular derived chondrocytes for cartilage reconstruction. (2013)
  • Author(s): Mizuno M, Takebe T, Kobayashi S, Lee S, Jo YH, Lee JI, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Vascularization of primary pancreatic islet towards efficient transplantation. (2013)
  • Author(s): Takahashi Y, Takebe T, Enomura M, Tanaka H, Koike N, Sekine K, Ueno Y, Zheng Y, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Generation of islet like tissue with human vascular network. (2013)
  • Author(s): Enomura M, Takebe T, Takahashi Y, Tanaka H, Koike N, Sekine K, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Flow cytometric identification and characterization of endothelial progenitor cells in human umbilical vein endothelial cells. (2013)
  • Author(s): Zheng Y, Aoki K, Tsuchida T, Zhang R, Sekine K, Ogawa M, Takebe T, Yasumura K, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Generation of human hepatocyte-like cells from human inducted pluripotent stem cells. (2013)
  • Author(s): Zhang R, Takebe T, Sekine K, Zheng Y, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Fluorescence visualization of human induced pluripotent stem cells using transcription activator-like (TAL) effector nucleases. (2013)
  • Author(s): Sekine K, Takebe T, Zhang R, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Effects of natural cartilage extracellular matrix on chondrogenic potential and dedifferentiation for autologous chondrocyte transplantation (2013)
  • Author(s): Yang W, Lee S, Jo YH, Nemeno JGE, Yeo JE, Nam BM, Kim BY, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] 3D scaffold-free spheroids fabricated from chondrocytes, synovium-derived, and bone marrow-derived mesenchymal stem cells (BM-MSCs) (2013)
  • Author(s): Nemeno JGE, Jo YH, Lee S, Yang W, Nam BM, Kim BY, Yeo JE, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Effect of cryopreservation and cell passaging on cell preparation for autologous chondrocyte transplantation. The 13th Congress of the Asian Society of Transplantation (2013)
  • Author(s): Nam BM, Kim BY, Lee S, Jo YH, Nemeno JGE, Yeo JE, Yang W, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Effect of different preservation conditions on cartilage tissue for cell transplantation. (2013)
  • Author(s): Kim BY, Nam BM, Jo YH, Lee S, Jo YH, Nemeno JGE, Yeo JE, Yang W, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Assessment of animal pancreatic islets after transportation between Korea and Japan. (2013)
  • Author(s): Lee S, Mizuno M, Jo YH, Yang W, Nemeno JGE, Yeo JE, Nam BM, Kim BY, Takebe T, Taniguchi H, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Artificial islet of hybrid cellular spheroids using three different cell lines. (2013)
  • Author(s): Jo YH, Nam BM, Kim BY, Lee S, Nemeno JGE, Yeo JE, Takebe T, Kim YS, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Vascularized and functional human liver from an induced pluripotent stem cell-derived organ bud transplant. (2013)
  • Author(s): Takebe T, Sekine K, Enomura M, Suzuki Y, Koike H, Zhang R, Koike N, Ueno Y, Zheng Y, Taniguchi H
  • Organizer: International Society for Stem Cell Research 11th annual meeting
  • Place of Presentation: Boston, USA
• [Presentation] Transient vascularization directs intrinsic cartilage formation from human progenitors. (2013)
  • Author(s): Takebe T, Kobayashi S, Mizuno M, Suzuki H, Chang Yu-Min, Yoshizawa E, Maegawa J, Taniguchi H
  • Organizer: International Society for Stem Cell Research 11th annual meeting
  • Place of Presentation: Boston, USA
• [Presentation] Liver repopulation in a diphtheria toxin-induced liver damage model with human proliferative hepatocytes. (2013)
  • Author(s): Zhang R, Zheng Y, Takebe T, Tsuchida T, Li B, Ueno Y, Taniguchi H
  • Organizer: International Society For Stem Cell Research 11th Annual Meeting
  • Place of Presentation: Boston, USA
• [Presentation] In vitro expansion of self-renewing hepatic progenitor cells by controlling metabolic state (2013)
  • Author(s): Koike H, Takebe T, Ueno Y, Taniguchi H
  • Organizer: International Society for Stem Cell Research 11th annual meeting
  • Place of Presentation: Boston, USA
• [Presentation] iPS細胞からヒト臓器を創る (2013)
  • Author(s): 武部貴則
  • Organizer: 横浜市立大学 細胞シグナリング研究会
  • Place of Presentation: 横浜市立大学（神奈川県）
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe
  • Organizer: THE 12TH US-JAPAN SYMPOSIUM ON DRUG DELIVERY SYSTEMS
  • Place of Presentation: Hawaii, USA
  • Invited
• [Presentation] iPS細胞からヒト臓器を創る (2013)
  • Author(s): 武部貴則
  • Organizer: 東京大学医科学研究所 若手シンポジウム
  • Place of Presentation: 東京大学医科学研究所（東京都）
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe
  • Organizer: Small RNAs to Stem Cells & Epigenetic Reprogramming Asia-2013 Meeting
  • Place of Presentation: University of Tokyo Hongo Campus, Tokyo, Japan
  • Invited
• [Presentation] iPS細胞からヒト臓器を創る (2013)
  • Author(s): 武部貴則
  • Organizer: 第16回日本異種移植研究会
  • Place of Presentation: 大阪国際会議場（大阪府）
  • Invited
• [Presentation] ヒトの臓器を創り出す！ (2013)
  • Author(s): 武部貴則
  • Organizer: Cell aggregation meeting 2013
  • Place of Presentation: ヒルトン福岡シーホーク（福岡県）
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe
  • Organizer: 4th Regenerative Medicine Seminar sponsored by JSPS and NRF
  • Place of Presentation: Konkuk University,Seoul, Korea
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe
  • Organizer: The 7th International Cell Therapy Conference
  • Place of Presentation: Seoul, Korea
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe
  • Organizer: 4th Regenerative Medicine Seminar sponsored by JSPS and NRF
  • Place of Presentation: Inha University, Seoul, Korea
  • Invited
• [Presentation] iPS細胞からヒト臓器を創る (2013)
  • Author(s): 武部貴則
  • Organizer: 東京女子医科大学 先端生命医科学研究所 グローバルCOEプログラム セミナー
  • Place of Presentation: 東京女子医科大学（東京都）
  • Invited
• [Presentation] 健やかなミライを実現するための全方位的アプローチ (2013)
  • Author(s): 武部貴則
  • Organizer: 倶進会東京支部 セミナー
  • Place of Presentation: 八芳園（東京都）
  • Invited
• [Presentation] Nature Medicine special project (2013)
  • Author(s): Takanori Takebe
  • Organizer: Herrenhausen Symposium on Stem Cells and Regenerative Medicine
  • Place of Presentation: Hannover, Germany
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe
  • Organizer: The 12th Congress International Xenotransplantation Association
  • Place of Presentation: 大阪国際会議場（大阪府）
  • Invited
• [Presentation] 如何にして器官発生を模倣するのか？ー血管系を有するヒト肝組織創出法の開発ー． (2013)
  • Author(s): 武部貴則
  • Organizer: 第12回日本再生医療学会 パネルディスカッション「再生組織に対する血管網付与」
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Invited
• [Presentation] ヒト耳介由来軟骨前駆細胞を用いた弾性軟骨再構築法の開発． (2013)
  • Author(s): 武部貴則
  • Organizer: 第12回日本再生医療学会 シンポジウム「組織幹細胞の最前線」
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Invited
• [Presentation] Reconstitution of Articular(joint) cartilage defects by auricle(ear) derived stem/ progenitor cells (2012)
  • Author(s): Mizuno M, Kobayashi S, Takebe T, Suzuki H, Murata S, Yabuki Y, Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting
  • Place of Presentation: Yokohama, Japan
• [Presentation] ヒト耳介軟骨膜由来幹/前駆細胞を用いた弾性軟骨再構築法の開発 (2012)
  • Author(s): 武部貴則、小林眞司、矢吹雄一郎、鈴木啓、水野満、安村和正、広富浩一、鄭允文、前川二郎、谷口英樹
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Creation of vascularized human organ from induced pluripotent stem cells. (2012)
  • Author(s): Takebe T
  • Organizer: International Society for Stem Cell Research 10th annual meeting
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Generation of islet like structures with human functional vascular networks. (2012)
  • Author(s): Enomura M
  • Organizer: International Society for Stem Cell Research 10th annual meeting
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Self-renewal versus differentiation as well as the liver repopulation capability of human hepatic stem cells. (2012)
  • Author(s): Zheng Y
  • Organizer: International Society for Stem Cell Research 10th annual meeting
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Construction of chimeric mice with human immatured hepatocytes. (2012)
  • Author(s): Zhang R
  • Organizer: International Society for Stem Cell Research 10th annual meeting
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Identification of pancreatic stem/progenitor cells expressing PDX1 reside in the CD133 positive pancreatic duct. (2012)
  • Author(s): Sekine K
  • Organizer: International Society for Stem Cell Research 10th annual meeting
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Reconstitution of Articular(joint) cartilage defects by auricle(ear) derived stem/ progenitor cells. (2012)
  • Author(s): Mizuno M
  • Organizer: International Society for Stem Cell Research 10th annual meeting
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出 (2012)
  • Author(s): 武部貴則
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] ヒト耳介軟骨膜由来幹／前駆細胞を用いた弾性軟骨再構築法の開発 (2012)
  • Author(s): 武部貴則
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出． (2012)
  • Author(s): 武部貴則
  • Organizer: 第39回日本臓器保存生物医学会学術集会 シンポジウム
  • Place of Presentation: コラッセふくしま（福島県）
  • Invited
• [Presentation] ヒトiPSを用いたファーマコセロミクス基盤技術の開発
  • Author(s): 谷口英樹
  • Organizer: 第127回日本薬理学会関東部会 シンポジウム
  • Place of Presentation: 東京国際フォーラム（東京都）
• [Presentation] Creation of vascularized human organ from induced pluripotent stem cells.
  • Author(s): Takanori Takebe
  • Organizer: 2nd Regenerative Medicine Seminar
  • Place of Presentation: Seoul, Korea
  • Invited
• [Presentation] Generation of human liver tissue from an induced pluripotent stem cell-derived organ bud transplant.
  • Author(s): Takanori Takebe
  • Organizer: Symposium “Current state and future of Regenerative Medicine”, The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: タワーホール船堀（東京都）
  • Invited
• [Presentation] iPS細胞由来肝原基移植に基づく機能的なヒト肝組織の創出.
  • Author(s): 武部貴則
  • Organizer: 第53回幹細胞治療研究センターフォーラム
  • Place of Presentation: 東京大学医科学研究所（東京都）
  • Invited
• [Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出．
  • Author(s): 武部貴則
  • Organizer: ERATO竹内バイオ融合プロジェクト 第１回 奨励会特別研究委員会
  • Place of Presentation: 東京大学生産技術総合研究所（東京都）
  • Invited
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~saisei/
• [Patent(Industrial Property Rights)] 軟骨細胞の調製方法 (2014)
  • Inventor(s): 武部貴則(50%),谷口英樹(35%),小林眞司(15%)
  • Industrial Property Rights Holder: 武部貴則(50%),谷口英樹(35%),小林眞司(15%)
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2014-03-20
  • Overseas
• [Patent(Industrial Property Rights)] 自己組織化用細胞集合体の作製方法 (2014)
  • Inventor(s): 武部貴則, 吉川洋史, 谷口英樹
  • Industrial Property Rights Holder: 武部貴則, 吉川洋史, 谷口英樹
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-037341
  • Filing Date: 2014-02-27
• [Patent(Industrial Property Rights)] 軟骨細胞の調製方法 (2014)
  • Inventor(s): 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Rights Holder: 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-21
• [Patent(Industrial Property Rights)] 生物学的組織に血管系を付与する方法 (2013)
  • Inventor(s): 武部貴則, 谷口英樹, 高橋禎暢
  • Industrial Property Rights Holder: 武部貴則, 谷口英樹, 高橋禎暢
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-07-23
• [Patent(Industrial Property Rights)] 生物学的組織に血管系を付与する方法 (2013)
  • Inventor(s): 武部貴則, 谷口英樹, 高橋禎暢
  • Industrial Property Rights Holder: 武部貴則, 谷口英樹, 高橋禎暢
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-153056
  • Filing Date: 2013-07-23
• [Patent(Industrial Property Rights)] 組織構造体及びその作製方法 (2013)
  • Inventor(s): 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Holder: 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-122190
  • Filing Date: 2013-06-10
• [Patent(Industrial Property Rights)] 培養容器及び培養方法 (2013)
  • Inventor(s): 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Holder: 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-120915
  • Filing Date: 2013-06-07
• [Patent(Industrial Property Rights)] アミノ酸製剤による細胞増幅法 (2013)
  • Inventor(s): 谷口英樹, 武部貴則, 上野康晴、小池博之
  • Industrial Property Rights Holder: 谷口英樹, 武部貴則, 上野康晴、小池博之
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-106289
  • Filing Date: 2013-05-21
• [Patent(Industrial Property Rights)] 軟骨細胞の調製方法 (2013)
  • Inventor(s): 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Rights Holder: 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-058534
  • Filing Date: 2013-03-21
• [Patent(Industrial Property Rights)] ヒト組織・臓器の作成方法 (2012)
  • Inventor(s): 谷口英樹,武部貴則
  • Industrial Property Rights Holder: 谷口英樹,武部貴則
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-09-28
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