| Project/Area Number |
24659785
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Hideki 横浜市立大学, 医学研究科, 教授 (70292555)
KOBAYASHI Shinji 横浜市立大学, 医学研究科, 客員准教授 (90464536)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 移植・再生医療 / 再生医学 |
|---|
| Research Abstract |
Here, we report that avascular elastic cartilage could be efficiently regenerated from in vitro grown mesenchymal condensation by recapitulating early chondrogenesis. Combined with an intravital imaging approach, we identified the presence of endothelial cells inside the mesenchymal condensation prior to cartilage maturation. Strikingly, recreating endothelial interaction in culture enabled the recently identified adult progenitors to generate a mesenchymal condensation in vitro in a self-driven manner without any support of exogenous factors nor scaffolds. These in vitro grown 3-D condensed progenitors much efficiently reconstructed three-dimensional elastic cartilage upon transplantation compared with conventional methods. Thus, the transplantation of condensed progenitors with endothelial cells represents a promising new approach towards realizing regenerative medicine as well as studying the mechanism of vascular regression.
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