Elucidation of the in vivo migration of mesenchymal stem cells.
| Project/Area Number |
24659789
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Tokyo Medical University (2013)
Keio University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 間葉系幹細胞 / 創傷治癒学 |
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| Research Abstract |
We report an improved prospective clonal isolation technique and reveal that the combination of three cell-surface markers (LNGFR, THY-1, and VCAM-1) allows for the selection of highly enriched clonogenic cells (one out of three isolated cells). Clonal characterization of LNGFR+THY-1+ cells demonstrated cellular heterogeneity among the clones. Rapidly expanding clones (RECs) exhibited robust multilineage differentiation and self-renewal potency, whereas the other clones tended to acquire cellular senescence via P16INK4a and exhibited frequent genomic errors. Furthermore, RECs exhibited unique expression of VCAM-1 and higher cellular motility compared with the other clones. The combination marker LNGFR+THY-1+VCAM-1hi+ (LTV) can be used selectively to isolate the most potent and genetically stable MSCs.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Homeodomain transcription factor meis1 is a critical regulator of adult bone marrow hematopoiesis.2014
Author(s)
Ariki R, Morikawa S, Mabuchi Y, Suzuki S, Nakatake M, Yoshioka K, Hidano S, Nakauchi H, Matsuzaki Y, Nakamura T, Goitsuka R.
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Journal Title
PLoS One.
Volume: 9
Issue: 2
Pages: 87646-87646
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Twist2 functions as a tumor suppressor in murine osteosarcoma cells.2013
Author(s)
Ishikawa T, Shimizu T, Ueki A, Yamaguchi S, Onishi N, Sugihara E, Kuninaka S, Miyamoto T, Morioka H, Nakayama R, Kobayashi E, Toyama Y, Mabuchi Y, Matsuzaki Y, Yamaguchi R, Miyano S and Saya H
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Journal Title
Cancer Sci
Volume: (in press)
Issue: 7
Pages: 880-888
DOI
Related Report
Peer Reviewed
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[Journal Article] Transplantation of side population cells restores the function of damaged exocrine glands through clusterin2012
Author(s)
Mishima K., Inoue H., Nishiyama T., Mabuchi Y., Amano Y., Ide F., Matsui M., Yamada H., Yamamoto G., Tanaka J., Yasuhara R., Sakurai T., Lee M., Chiba K., Sumimoto H., Kawakami Y., Matsuzaki Y., Tsubota K., Saito I
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Journal Title
Stem Cells
Volume: 30
Issue: 9
Pages: 1925-37
DOI
Related Report
Peer Reviewed
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