| Project/Area Number |
24659802
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Emergency medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
TANAKA Hiroshi 順天堂大学, 医学(系)研究科(研究院), 教授 (90252676)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Yoshiaki 順天堂大学, 医学部, 先任准教授 (60379196)
SUMI Yuka 順天堂大学, 医学部, 准教授 (40403084)
SUGINAKA Hiroshi 順天堂大学, 医学部, 助手 (30510424)
MORIKAWA Miki 順天堂大学, 医学部, 助手 (40621892)
ISHIKAWA Kohei 順天堂大学, 医学部, 助手 (50621900)
IWABUCHI Kazuhisa 順天堂大学, 医療看護学部, 教授 (10184897)
ARAKI Yoshihiko 順天堂大学, 大学院, 准教授 (70250933)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 多臓器不全 / 敗血症 / DAMPｓ / ATP / 白血球機能 / 蛍光イメージング / 心肺停止蘇生後 / DIC / DAMPs |
|---|
| Research Abstract |
ATP is involved in both intra and extracellular signaling. Neutrophils (PMNs) have a pivotal role in inflammation response. In sepsis patients, plasma ATP levels and CD11b were significantly higher than healthy controls, and the CD11b correlates with plasma ATP. These data suggest that ATP release from damaged cells or activated inflammatory cells stimulates PMNs via ATP receptors and triggers inflammation in sepsis. Sepsis-induced DIC is associated with a high mortality rate. The function and deformability of PMN change in patients with sepsis induced DIC was evaluated. We clarified that PMN deformability and activation correlated with the severity of sepsis-induced DIC. Fluorescence imaging of ATP in human PMNs with chemosensors (PMAP-1, MitoAP-1) was established. This method would contribute to understanding the dynamics of ATP in PMNs and elucidating their diverse physiological functions in inflammation.
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