| Project/Area Number |
24659811
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Mie University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMANE Toshiyuki 三重大学, 大学院医学系研究科, 准教授 (30452220)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 神経堤細胞 / 中胚葉 / ジフテリアトキシン / 歯髄 / 造血支持能 / 胸腺 / 骨髄 / 骨髄形成 / 間葉細胞 |
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| Outline of Final Research Achievements |
By using Cre-LoxP mediated transgenic mice that have NC- or mesoderm-specific Cre expression and Cre-activated YFP reporter and Diphtheria Toxin (DT) Receptor, NC- or mesoderm-derived cells were depleted by the administration of DT. The consequence of DT-mediated depletion of these cells on B and T lymphopoiesis was examined by flowcytometry. Severe reduction of the number of B and T cell progenitors in the BM and thymus was observed by the depletion. As NC cells contributed to sympathetic neurons, to investigate the affect of sympathetic neurons in DT-administrated mice, concentrations of catechoramin were measured in plasma, and those of noradrenalin and adrenalin were significantly reduced. These results suggest that NC-derived mesenchyme and sympathetic neurons are involved in B and T lymphopoiesis. Although dental mesenchyme supported B lymphopoiesis and osteoclastogenesis as well as bone marrow, embryonic tooth mesenchyme had little hematopoietic supporting capacity.
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