Establishment of a novel screening system to isolate PTH-like osteogenic small compounds
| Project/Area Number |
24659824
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
NISHIMURA Riko 大阪大学, 歯学研究科(研究院), 教授 (60294112)
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| Co-Investigator(Kenkyū-buntansha) |
HATA Kenji 大阪大学, 大学院歯学研究科, 准教授 (80444496)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | PTH / 骨形成 / スクリーニング / 骨芽細胞 |
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| Research Abstract |
Parathyroid hormone (PTH) has been shown to be a effective therapeutic agents for bone diseases such as osteoporosis. However, PTH has two major problems; high cost and limitation of term due to its possible side effect. Thus, we have attempted to contribute to development of screening systems that can allow us to explore the small compounds to mimic osteogenic action of PTH. We found that an osteoblastic cell line, SaOS2, is useful and suitable for the screening system. We also attempted to identify the target genes of PTH. Our data suggest that Wnt5a would be a target gene of PTH during bone formation. Our study suggest that a screening system in which we can determine the Wnt5a gene promoter activity would be helpful for developing the therapeutic bone forming small compounds.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] ZFHX4 interacts with the NuRD core member CHD4 and regulates the glioblastoma tumor initiating cell state2013
Author(s)
Chudnovsky Y, Kim D, Zheng S, Whyte WA, Bansal M, Bray M, Gopal S, Theisen MA, Bilodeau S, Thiru P, Muffat J, Yilmaz OH, Mitalipova M, Woolard K, Lee J, Nishimura R, Sakata N, Fine HA, Carpenter AE, Silver SJ, Verhaak RGW, Califano A, Young RA, Ligon KL, Mellinghoff IK, Root DE, Sabatini DM, Hahn WC, Chheda MG
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Journal Title
Cell Reports
Volume: 6
Pages: 1-12
Related Report
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[Journal Article] Arid5b facilitates chondrogenesis by recruiting the histone demethylase Phf2 to Sox9-regulated genes2013
Author(s)
Hata K, Takashima R, Amano K, Ono K, Nakanishi M, Yoshida M, Wakabayashi M, Matsuda A, Maeda Y, Suzuki Y, Sugano S, Whitson RH, Nishimura R and Yoneda T
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Journal Title
Nature communications
Volume: 4
Issue: 1
Pages: 2850-2860
DOI
Related Report
Peer Reviewed
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[Journal Article] ZFHX4 interacts with the NuRD core member CHD4 and regulates the glioblastoma tumor initiating cell state.2013
Author(s)
Chudnovsky Y, Kim D, Zheng S, Whyte WA, Bansal M, Bray M, Gopal S, Theisen MA, Bilodeau S, Thiru P, Muffat J, Yilmaz OH, Mitalipova M, Woolard K, Lee J, Nishimura R, Sakata N, Fine HA, Carpenter AE, Silver SJ, Verhaak RGW, Califano A, Young RA, Ligon KL, Mellinghoff IK, Root DE, Sabatini DM, Hahn WC, Chheda MG
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Journal Title
Cell Reports
Volume: 6
Issue: 2
Pages: 1-12
DOI
Related Report
Peer Reviewed
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