| Project/Area Number |
24659840
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TSUKUBA Takayuki 長崎大学, 医歯(薬)学総合研究科, 教授 (30264055)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Kuniaki 長崎大学, 大学院医歯薬学総合研究科, 准教授 (10311846)
SAKAI Eiko 長崎大学, 大学院医歯薬学総合研究科, 助教 (10176612)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ライブイメージング / 歯周病細菌 / バイオイメージング / 歯周病菌 |
|---|
| Research Abstract |
We analyzed a fate of Porphyromonas gingivalis, a periodontal pathogen, with human aortic endothelial cells by live-imaging. We detected the bacteria within the cells for 6-8 hs, but failed to find them for 18-24 hs, indicating that the bacteria is probably transported to the lysosomes.
Futhermore, we performed live-imaging of P. gingivalis using mice. We found that the bacteria attached on blood vessels of bifurcation area and thin vessels. In ApoE-deficient mice, which is a model for arterial sclerosis, the attachment rate of the bacteria was increased. These results indicate that cholesterol in the blood probably interacts with P. gingivalis during endocytosis.
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