Role of SPARC on tumor metastasis as the transcription inhibitory factor

• Project/Area Number: 24659842
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Ohu University
• Principal Investigator: KATO Yasumasa 奥羽大学, 歯学部, 教授 (50214408)
• Co-Investigator(Renkei-kenkyūsha): KIMIHARU Hirose 奥羽大学, 歯学部, 教授 (10218836)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: SPARC / AP-1 / 転写調節 / 転移

Outline of Final Research Achievements

Roles of SPARC/osteonectin on tumor growth, invasion, and metastasis, are still controversial. Because AP-1 plays common role of following gene expression, Camp, Ngp, and Saa3, whose expression was up-regulated in SPARC knockout mice, we focused on AP-1 as SPARC target to be down-regulated. AP-1 activity was upregulated by SPARC gene knockout and inhibited by introduction of SPARC expression vector having signal peptide of preprotrypsin as the secreted form. Similar results were found when we used signal peptide-deleted expression vector to induce SPARC expression as the intracellular protein. These results suggested that SPARC plays a role of AP-1 inhibition as the intracellular function.

Research Products

• [Journal Article] Mineral trioxide aggregate induces osteoblastogenesis via Atf6 (2015)
  • Author(s): Maeda T, Suzuki A, Yuzawa S, Baba Y, Kimura Y, Kato Y
  • Journal Title: Bone Reports Volume: 2 Pages: 36-43
  • DOI: 10.1016/j.bonr.2015.03.003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Suppressed rate of carcinogenesis and decreases in tumour volume and lung metastasis in CXCL14/BRAK transgenic mice. (2015)
  • Author(s): Hata R, Izukuri K, Kato Y, Sasaki S, Mukaida N, Maehata Y, Miyamoto C, Akasaka T, Yang X, Nagashima Y, Takeda K, Kiyono T, Taniguchi M.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 9083-9083
  • DOI: 10.1038/srep09083
  • Peer Reviewed / Open Access
• [Journal Article] Deguelin Induced Apoptosis by Targeting both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines (2015)
  • Author(s): Baba Y, Fujii M, Maeda T, Suzuki A, Yuzawa S, Kato Y
  • Journal Title: BioMed Research International Volume: 2015 Pages: 1-9
  • DOI: 10.1155/2015/657179
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CRISPR / Cas9を用いたMC3T3-E1細胞におけるGPR81遺伝子のノックアウト (2014)
  • Author(s): 前田豊信、鈴木厚子、湯澤 仁、馬場 優、加藤靖正
  • Journal Title: 奥羽大学歯学誌 Volume: 41 Pages: 123-128
  • NAID: 120005759844
  • Peer Reviewed
• [Journal Article] Acidic extracellular pH promotes epithelial mesenchymal transition in Lewis lung carcinoma model (2014)
  • Author(s): Suzuki A, Maeda T, Baba Y, Shimamura K, Kato Y
  • Journal Title: Cancer Cell International Volume: 14 Issue: 1 Pages: 129-129
  • DOI: 10.1186/s12935-014-0129-1
  • Peer Reviewed / Open Access
• [Journal Article] Acidic extracellular microenviromental and cancer (2013)
  • Author(s): Kato Y, Ozawa S, Miyamoto C, Maehata Y, Suzuki A, Maeda T, Baba Y
  • Journal Title: Cancer Cell International Volume: 13(1) Issue: 1 Pages: 89-89
  • DOI: 10.1186/1475-2867-13-89
  • Peer Reviewed
• [Journal Article] Levocetirizine Hydrochloride Tablet Improves the Nasal Conditions of Patients with Cedar Pollen Allergy and Maintains their QOL (2013)
  • Author(s): Baba Y, Yoshihama K, Nishiyama T, Kato Y.
  • Journal Title: Clinical and Experimental Medical Sciences Volume: 2013 Pages: 329-341
  • Peer Reviewed
• [Journal Article] Vocal cord actinomycosis mimicking a laryngeal tumor (2013)
  • Author(s): Yoshihama K, Kato Y, and Baba Y
  • Journal Title: Case Reports in Otolaryngology Volume: 2013 Pages: 1-2
  • DOI: 10.1155/2013/361986
  • Peer Reviewed
• [Journal Article] Fasudil Suppresses Fibrosarcoma Growth by Stimulating Secretion of the Chemokine CXCL14/BRAK (2012)
  • Author(s): Miyamoto C, Maehata Y, Ozawa S., Ikoma T, Kubota E, Izukuri K, Kato Y, Hata R, Lee M-C
  • Journal Title: Journal of Pharmacological Sciences Volume: 120 Issue: 3 Pages: 241-249
  • DOI: 10.1254/jphs.12177FP
  • NAID: 10031147570
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Xanthogranuloma of the external auditory canal mimicking a benign tumor (2012)
  • Author(s): Yoshihama K, Kato Y, and Baba Y
  • Journal Title: Case Reports in Otolaryngology Volume: 2012 Pages: 1-3
  • DOI: 10.1155/2012/298089
  • Peer Reviewed
• [Journal Article] Present and future of EGFR inhibitors for head and neck squamous cell cancer (2012)
  • Author(s): Baba Y, Fujii M, Tokumaru Y, Kato Y
  • Journal Title: Journal of oncology Volume: Volume 2012(Article ID 986725) Pages: 9-9
  • DOI: 10.1155/2012/986725
  • Peer Reviewed
• [Journal Article] Cetuximab for Head and Neck Cancer from bench to clinic (2012)
  • Author(s): Baba Y, Fujii M, Tokumaru Y, Kato Y
  • Journal Title: Journal of Solid Tumors Volume: Vol. 2 Issue: 6 Pages: 16-22
  • DOI: 10.5430/jst.v2n6p16
  • Peer Reviewed
• [Presentation] MTAは骨芽細胞様細胞でATF6を介して石灰化を促進させる (2014)
  • Author(s): 前田豊信、鈴木厚子、湯澤仁、阿部匡聡、加藤靖正
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] MC3T3-E1細胞において、MTAはATF6を介して石灰化を促進させる (2014)
  • Author(s): 前田豊信、鈴木厚子、湯澤仁、加藤靖正
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Expression of chemokine CXCL14/BRAK whether in melanoma cells or in non-tumor cells in their environment suppresses metastasis to the lung. (2014)
  • Author(s): Hata R, Izukuri K, Kato Y, Maehata Y, Miyamoto C, Akasaka T, Kiyono T.
  • Organizer: 15th International Biennial Congress the Metastasis Research Society
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2014-06-28 – 2014-07-01
• [Presentation] Neutrophlic granule proteins (Ngp) is a SPARC target gene to be reduced (2014)
  • Author(s): Kato Y, Maeda T, Suzuki A, Yuzawa S, Baba Y, Miyamoto C, Maehata Y, Ozawa S, Kubota E, Hata R
  • Organizer: 15th International Biennial Congress the Metastasis Research Society
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2014-06-28 – 2014-07-01
• [Presentation] Mineral Trioxide Aggregateセメントの骨形成作用 (2014)
  • Author(s): 前田豊信、鈴木厚子、湯澤 仁、馬場優、木村裕一, 加藤靖正
  • Organizer: 第57回奥羽大学歯学会
  • Place of Presentation: 郡山
  • Year and Date: 2014-06-21
• [Presentation] 微小環境が発癌、癌の進展を制御する：癌に強いマウスの作製. (2014)
  • Author(s): 畑隆一郎、居作和人、加藤靖正、前畑洋次郎、宮本千央、赤坂徹、佐々木宗一郎、向田直史
  • Organizer: 第46回日本結合組織学会学術大会/第61回マトリックス研究会大会 合同学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-06-05 – 2014-06-07
• [Presentation] 酸性細胞外pHは，がん細胞の悪性形質を促進する微小環境因子である (2014)
  • Author(s): 加藤靖正
  • Organizer: アクリジンオレンジ研究会
  • Place of Presentation: 京都
  • Invited
• [Presentation] PubMedを過信してはいけない (2013)
  • Author(s): 畑隆一郎, 居作和人, 加藤靖正
  • Organizer: 第４５回日本結合組織学会学術大会・第６０回マトリックス研究会合同学術大会
  • Place of Presentation: 和歌山
• [Presentation] 上皮間葉系移行における酸性細胞外pHの急性影響と慢性影響 (2013)
  • Author(s): 加藤靖正
  • Organizer: 第２２回日本がん転移学会学術集会・総会
  • Place of Presentation: 松本
• [Presentation] 酸性細胞外pHへの順化は，酸性細胞外pH刺激の感受性を増加し，MMP9の産生能と運動能を促進する (2013)
  • Author(s): 加藤靖正，鈴木厚子，前田豊信
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 癌に強くなる遺伝子の発見 (2013)
  • Author(s): 畑隆一郎，居作和人，加藤靖正、佐々木宗一郎、向田直史
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] ケモカインCXCL14/BRAKは多段階癌抑制分子である (2013)
  • Author(s): 畑隆一郎，居作 和人，加藤靖正
  • Organizer: 第５５回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
• [Presentation] 腫瘍の悪性形質に対する酸性細胞外pHの急性および慢性影響 (2013)
  • Author(s): 加藤靖正
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Adaptation to acidic extracellular pH induces epithelial-mesenchymal transition as stable phenotype in Lewis lung carcinoma model (2013)
  • Author(s): Kato, Y. 1, Suzuki, A. 1, Maeda, T. 1, and Baba, Y.
  • Organizer: The 4th Annual Meeting of the International Society for Proton Dynamics in Cancer (ISPDC)
  • Place of Presentation: Munich, Germany
• [Presentation] Tumor-Suppressing Chemokine CXCL14/BRAK Is a Cell-Surface Associated Matrix Component (2013)
  • Author(s): Hata RI, Izukuri K, and Kato Y.
  • Organizer: 9th Pan Pacific Connective Tissue Societies Symposium (9th PPCTSS)
  • Place of Presentation: Hong Kong
• [Presentation] Epithelial-mesenchymal transition and extracellular acidic pH. (2012)
  • Author(s): Kato, Y., Suzuki, A., Maeda, T., and Shimamura, K.
  • Organizer: 3rd Symposium of the International Society for Proton Dynamics in Cancer
  • Place of Presentation: 京都府立医大
• [Presentation] Acidic extracellular pH induces epithelial-mesenchymal transition in Lewis lung carcinoma model. (2012)
  • Author(s): Kato, Y., Suzuki, A., Maeda, T., and Shimamura, K.
  • Organizer: 14th International Biennial Conference on Metastasis Research 2012
  • Place of Presentation: Meruborune
  • Invited
• [Presentation] 酸性細胞外pHは、ルイス肺癌細胞の上皮間葉移行を誘導する． (2012)
  • Author(s): 加藤靖正
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Invited
• [Presentation] CXCL14/BRAKの発現は癌の増殖と転移を抑制し担癌マウスの寿命を延長する． (2012)
  • Author(s): 畑隆一郎，加藤靖正，竹田和由，清野 透，向田直史，谷口 克
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Invited
• [Book] New strategy in head and neck cancer: combination therapy targeting the PI3kinase/Akt and EGFR signaling pathways. In: Hypothesis in clinical medicine (2012)
  • Author(s): Baba, Y., Fujii, M., Tokumaru, Y., and Kato, Y.
  • Total Pages: 18
  • Publisher: Nova Science Publishers, Inc. (ISBN: 978-1-62257-285-4)
• [Book] Cell Signalings and the Communications in Head and Neck Cancer, In: Head and neck cancer . (2012)
  • Author(s): Baba, Y., Fujii, M., Tokumaru, Y., and Kato, Y..
  • Total Pages: 17
  • Publisher: In Tech–Open Access Publisher, (ISBN 978-953-51-0236-6, DOI: 10.5772/1246)
• [Book] New strategy in head and neck cancer: combination therapy targeting the PI3kinase/Akt and EGFR signaling pathways. In: Hypothesis in clinical medicine (2012)
  • Author(s): Baba, Y., Fujii, M., Tokumaru, Y., and Kato, Y.
  • Total Pages: 18
  • Publisher: Nova Science Publishers (ISBN: 978-1-62257-285-4)