| Project/Area Number |
24659847
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NISHITANI Yoshihiro 岡山大学, 大学院医歯薬学総合研究科, 准教授 (60325123)
OHARA Naoko 岡山大学, 大学院医歯薬学総合研究科, 助教 (80301365)
OHARA Naoya 岡山大学, 大学院医歯薬学総合研究科, 教授 (70223930)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 歯学 / 歯科保存修復学 / 細菌学 / 接着歯学 |
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| Research Abstract |
We used Corynebacterium matruchotii as test bacteria, and we cultured bacteria to the agar medium and a liquid medium which added calcium chloride. We set a period of an instruction and the mineral formation with 2-12 months and observed the formation of the calcium deposition.
As a result, we observed the mineral formation in the liquid culture from two months, and the calcification in the cell body became clear with the increase between the mineral formative periods. We did not accept the clear calcium deposition formation in the solid culture. C. matruchotii produced bacterial biomineralization by culturing it in calcium-rich environment. In addition, we got a conclusion that the instruction in the liquid medium is desirable for an instruction method.
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