Could human dental pulp stem cell be an alternative cell source for periodontal tissue regeneration of reimplanted tooth?

• Project/Area Number: 24659848
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: WADA Naohisa 九州大学, 大学病院, 講師 (60380466)
• Co-Investigator(Kenkyū-buntansha): AKAMINE Akifumi 九州大学, 歯学研究院, 教授 (00117053) ; MAEDA Hidefumi 九州大学, 大学病院, 講師 (10284514) ; MONNOUCHI Satoshi 九州大学, 歯学研究院, 助教 (30609558)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 歯髄幹細胞 / 歯周組織 / 歯根膜組織 / 組織再生

Research Abstract

In this study, we hypothesized that human dental pulp stem cells (hDPSC) could possess the potential to convert to periodontal ligament cell-like cells. We isolated the cell population, which showed the expression of mesenchymal stem cell markers and multipotency to differentiate into various cell types, from human dental pulp tissue. We investigated the PDL-related gene expression in this cell population, which could be hDPSC, cultivated on bone slices or with recombinant TGF-beta1 addition. hDPSC cultured on bone slices for 2 weeks enhanced gene expression of Periostin, Col-1, alpha-SMA at the levels similar to PDLSC. Moreover, TGF-beta1 addition also upregulated these gene expression in hDPSC. These findings suggested that DPSCs contacting to bone tissue and stimulated with TGF-beta1 could convert to PDL-like cells. Although further studies are required to clarify the precise differentiation potential, hDPSC can be alternative cell source to regenerate PDL tissue efficiently.

Research Products

• [Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells (2014)
  • Author(s): Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
  • Journal Title: Stem Cells Dev Volume: (Epub ahead of print) Issue: 18 Pages: 2225-2236
  • DOI: 10.1089/scd.2013.0405
  • Peer Reviewed
• [Journal Article] Regeneration of the periodontium for preservation of the damaged tooth (2014)
  • Author(s): Maeda H, Tomokiyo A, Wada N, Koori K, Kawachi G, Akamine A
  • Journal Title: Histol Histopathol Volume: (Epub ahead of print)
  • URL: http://www.hh.um.es/Reviews-proofs/11-489-manuscript.pdf
• [Journal Article] Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines. (2013)
  • Author(s): Kono K
  • Journal Title: Cell Tissue Res. Volume: in press Issue: 2 Pages: 249-263
  • DOI: 10.1007/s00441-012-1543-0
  • Peer Reviewed
• [Journal Article] Immunomodulatory Effects of Stem Cells (2013)
  • Author(s): Wada N, Gronthos S, Bartold PM
  • Journal Title: Periodontol 2000 Volume: 63(1) Issue: 1 Pages: 198-216
  • DOI: 10.1111/prd.12024
  • Peer Reviewed
• [Journal Article] Prospective Potency of TGF-β1 on Maintenance and Regeneration of Periodontal Tissue (2013)
  • Author(s): Maeda H, Wada N, Tomokiyo A, Monnouchi S, Akamine A
  • Journal Title: Int Rev Cell Mol Biol Volume: 304 Pages: 283-367
  • DOI: 10.1016/b978-0-12-407696-9.00006-3
  • ISBN: 9780124076969
  • Peer Reviewed
• [Presentation] Semaphorin3Aがヒト歯髄幹細胞による硬組織形成に及ぼす影響 (2014)
  • Author(s): 吉田晋一郎、和田尚久、前田英史、門野内聡、長谷川大学、御手洗裕美、濱野さゆり、祐田明香、杉井英樹、赤峰昭文
  • Organizer: 第140回日本歯科保存学会春季学術大会
  • Place of Presentation: 滋賀県立芸術劇場(大津市)
• [Presentation] Semaphorin3Aがヒト歯髄幹細胞による硬組織形成に及ぼす影響 (2014)
  • Author(s): 吉田晋一朗、和田尚久、前田英史、門野内聡、長谷川大学、 御手洗裕美、濱野さゆり、祐田明香、杉井英樹、赤峰昭文
  • Organizer: 日本歯科保存学会春季学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール
• [Presentation] Dental pulp stem cells on bone tissue express periodontal ligament-related gene (2013)
  • Author(s): Yoshida S, Wada N, Maeda H, Hasegawa D, Sato H, Monnouchi S, Akamine A
  • Organizer: The 9th World Endodontic Congress, Tokyo International Forum
  • Place of Presentation: Tokyo, Japan
• [Presentation] Dental pulp stem cells on bone tissue express periodontal ligament-related gene. (2013)
  • Author(s): Yoshida S, Wada N, Maeda H, Hasegawa D, Sato H, Monnouchi S, Akamine A.
  • Organizer: The 9th World Endodontic Congress,
  • Place of Presentation: Tokyo International Forum
• [Presentation] Dental pulp stem cells on bone tissue express periodontal ligament-related gene (2013)
  • Author(s): Shinichiro Yoshida
  • Organizer: The 9th World Endodontic Congress
  • Place of Presentation: Tokyo International Forum
• [Book] Differentiation of Periodontal Ligament Stem /Progenitor Cells : Roles of TGF-β1. In : Stem Cells and Cancer Stem Cells, volume 4, Therapeutic Applications in Disease and Injury. Hayat MA (ed) (2012)
  • Author(s): Maeda H, Fujii S, Monnouchi S, Wada N, Akamine A
  • Publisher: Springer, Heidelberg, Germany