| Project/Area Number |
24659848
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
WADA Naohisa 九州大学, 大学病院, 講師 (60380466)
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| Co-Investigator(Kenkyū-buntansha) |
AKAMINE Akifumi 九州大学, 歯学研究院, 教授 (00117053)
MAEDA Hidefumi 九州大学, 大学病院, 講師 (10284514)
MONNOUCHI Satoshi 九州大学, 歯学研究院, 助教 (30609558)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 歯髄幹細胞 / 歯周組織 / 歯根膜組織 / 組織再生 |
|---|
| Research Abstract |
In this study, we hypothesized that human dental pulp stem cells (hDPSC) could possess the potential to convert to periodontal ligament cell-like cells. We isolated the cell population, which showed the expression of mesenchymal stem cell markers and multipotency to differentiate into various cell types, from human dental pulp tissue. We investigated the PDL-related gene expression in this cell population, which could be hDPSC, cultivated on bone slices or with recombinant TGF-beta1 addition. hDPSC cultured on bone slices for 2 weeks enhanced gene expression of Periostin, Col-1, alpha-SMA at the levels similar to PDLSC. Moreover, TGF-beta1 addition also upregulated these gene expression in hDPSC. These findings suggested that DPSCs contacting to bone tissue and stimulated with TGF-beta1 could convert to PDL-like cells. Although further studies are required to clarify the precise differentiation potential, hDPSC can be alternative cell source to regenerate PDL tissue efficiently.
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