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Assembly of recellularized-dental pulp models using stem cells and decellularized dental pulp matrix

Research Project

Project/Area Number 24659849
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionAichi Gakuin University

Principal Investigator

NAKAMURA HIROSHI  愛知学院大学, 歯学部, 教授 (40064878)

Co-Investigator(Kenkyū-buntansha) OZEKI Nobuaki  愛知学院大学, 歯学部, 講師 (70469005)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords脱細胞化 / 再細胞化 / ES細胞 / iPS細胞 / 骨格筋幹細胞
Research Abstract

The decellularized dental pulp-mimicking matrices (ddpm) was extracted and purified by 0.1% sodium dodecyl sulfate (SDS). After the cells were seeded on ddpm for 7 days, cells exhibited neural crest cell markers (FoxD3, Sox10), neural progenitor cell markers (Notch-1, Nestin), odontoblast differentiation markers (Dspp, Dmp-1, Enamelysin) and vascular endothelial cell markers (Flk-1, VE-cadherin) by reverse transcription (RT)-polymerase chain reaction (PCR).
we conclude that the parental stem cells retained a specification that following ddpm treatment shifted to platform differentiated to dental pulp-like specification.
Possible alternative stem cells from other tissues including skeletal muscle could potentially be adapted for tooth organ regeneration using ddpm and these new approaches for teeth regeneration needs to be explored.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results)

  • [Journal Article] Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction ofα1 integrin expression2014

    • Author(s)
      Nobuaki Ozeki, Makio Mogi, Hideyuki Yamaguchi, Taiki Hiyama, Rie Kawai, Naoko Hase, Kazuhiko Nakata, Hiroshi Nakamura, and Randall H. Kramer
    • Journal Title

      J Biol Chem

      Volume: (Epub ahead of print in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells2014

    • Author(s)
      Nobuaki Ozeki, Rie Kawai, Hideyuki Yamaguchi, Taiki Hiyama, Katsue Kinoshita, Naoko Hase, Kazuhiko Nakata, Ayami Kondo, Makio Mogi and Hiroshi Nakamura
    • Journal Title

      Exp Cell Res

      Volume: 323(1) Pages: 165-77

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction of α1 integrin expression2014

    • Author(s)
      Nobuaki Ozeki, Makio Mogi, Hideyuki Yamaguchi, Taiki Hiyama, Rie Kawai, Naoko Hase, Kazuhiko Nakata, Hiroshi Nakamura, and Randall H. Kramer
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 印刷中 Issue: 20 Pages: 14380-14391

    • DOI

      10.1074/jbc.m113.526772

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28–regulated proliferation of human osteoblast-like cells2014

    • Author(s)
      Nobuaki Ozeki, Rie Kawai, Hideyuki Yamaguchi, Taiki Hiyama, Katsue Kinoshita, Naoko Hase, Kazuhiko Nakata, Ayami Kondo, Makio Mogi, and Hiroshi Nakamura
    • Journal Title

      Experimental Cell Research

      Volume: 323 Pages: 165-177

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] マウスES細胞機能を制御する歯髄擬態マトリックスの開発2013

    • Author(s)
      尾関伸明, 山口秀幸, 檜山太希, 川合里絵, 中村洋
    • Organizer
      第11回日本再生歯科医学会学術大会・総会
    • Place of Presentation
      日本大学理工学部CSTホール
    • Year and Date
      2013-08-31
    • Related Report
      2013 Final Research Report
  • [Presentation] Odontogenic potential of iPS and ES cells using decellularized dental pulp matrix2013

    • Author(s)
      Ozeki N, Yamaguchi H, Kawai R, Nakata K, Nakamura H
    • Organizer
      第9回世界歯内療法会議,第34回日本歯内療法学会学術大会,第11回日韓合同歯内療法学会学術大会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2013-05-26
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスiPS細胞とES細胞を用いた歯髄再生における歯髄脱細胞化マトリックスの役割2013

    • Author(s)
      尾関伸明, 山口秀幸, 川合里絵, 茂木眞希雄, 中村洋
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスES細胞機能を制御する歯髄擬態マトリックスの開発2013

    • Author(s)
      尾関伸明, 山口秀幸, 檜山太希, 川合里絵, 中田和彦, 中村 洋
    • Organizer
      日本再生歯科医学会
    • Place of Presentation
      日本大学理工学部1号館CSTホール
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウスiPS細胞とES細胞を用いた歯髄再生における歯髄脱細胞化マトリックスの役割2013

    • Author(s)
      尾関伸明
    • Organizer
      第12回 日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 歯髄脱細胞化マトリックスを用いたiPS細胞とES細胞の象牙質分化能2012

    • Author(s)
      尾関伸明, 山口秀幸, 川合里絵, 中村洋
    • Organizer
      第10回日本再生歯科医学会学術大会・総会
    • Place of Presentation
      ニチイ学館神戸ポートアイランドセンター
    • Year and Date
      2012-09-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 歯髄脱細胞化マトリックスを用いたiPS細胞とES細胞の象牙質分化能2012

    • Author(s)
      尾関伸明
    • Organizer
      第10回 日本再生歯科医学会学術大会
    • Place of Presentation
      ニチイ学館(神戸)
    • Related Report
      2012 Research-status Report
  • [Presentation] 万能性幹細胞の象牙芽細胞分化における歯髄脱細胞化マトリックスの役割2012

    • Author(s)
      尾関伸明
    • Organizer
      日本歯科保存学会 2012年度秋季学術大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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