Function elucidation of the ALP in the pluripotent maintenance of pulpal stem cells and iPS cells and its application
| Project/Area Number |
24659866
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SUZUKI KUNIAKI 北海道大学, 歯学研究科(研究院), 教授 (40133748)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Toshiyuki 岐阜大学, 大学院医学(系)研究科, 教授 (50226172)
DEYAMA Yoshiaki 北海道大学, 大学院歯学研究科, 准教授 (80271667)
IIDA Kazuki 岐阜大学, 医学部附属病院, 助教 (30585237)
TAMAOKI Naritaka 岐阜大学, 大学院医学(系)研究科, 助教 (40585303)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ES細胞 / iPS細胞 / 多分化能 / アルカリ性ホスファターゼ / 臓器非特異型ALP / 細胞の初期化 / 腫瘍化 / 歯髄幹細胞 / 酵素阻害剤 / 酵素基質 |
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| Research Abstract |
We examined a property of the alkaline phosphatase (ALP) used for a pluripotent index in the study of embryonic stem cells and iPS cells. The dental pulp stem cells (DPSC) did not show ALP activity, but the induced iPS cells from DPSC showed the ALP activity of the tissue nonspecific type. As for the origin of the organ, the tumor cells which we used for this study showed all a characteristic of the tissue nonspecific type ALP. This suggests that the cells lost original type of the ALP by becoming a tumor, and came to show the nature of the tissue nonspecific type. As the above-mentioned results are interesting in an association between initialization of cells and malignant transformation and ALP, we continue the study.
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Report
(3 results)
Research Products
(20 results)
