Direct reprogramming to osteocytes by modulating insulator function
Project/Area Number |
24659870
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Dental engineering/Regenerative dentistry
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
IKEDA Masa-Aki 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (20193211)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | 分化転換 / リプログラミング / インスレーター / 骨細胞 / ARID3A / 繊維芽細胞 / 間葉系幹細胞 / 再生医療 / 骨 / 軟骨 / 培養 / 脂肪 / siRNA |
Outline of Final Research Achievements |
In this study, we tried to inhibit insulator functions by siRNA-mediated ARID3A silencing and thereby facilitate reprogramming. In order to convert human fibroblasts into osteocytes, we tested the effects of siRNA-mediated ARID3Asilencing and various combinations of small chemical compounds and growth factors on epigenetic reprogramming of fibroblasts. We found a combination of small chemical compounds and growth factors that directly converted fibroblasts into osteocytes and adipocytes without using siRNA-mediated ARID3A silencing.
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Report
(4 results)
Research Products
(16 results)
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[Presentation] Alpha-L-fucosidase (FUCA1) is a p53 target gene, and is expressed at low levels in anaplastic thyroid carcinomas2013
Author(s)
Tsuchida N, Ikeda MA, Kanazawa S, Ishino Y, Kaji K, Salvatore, G, Santoro M, Vecchio G
Organizer
18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine
Place of Presentation
Crete, Greece
Related Report
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