| Project/Area Number |
24659871
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University (2014)
Tokyo Medical and Dental University (2012-2013) |
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Principal Investigator |
NAKATA Hidemi 岩手医科大学, 歯学部, 非常勤講師 (30451967)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Shinji 東京医科歯科大学, 大学院医歯学総合研究科口腔機能再構築学講座インプラント・口腔再生医学分野, 助教 (50323689)
KONDO Hisatomo 岩手医科大学, 歯学部補綴・インプラント学講座, 教授 (70343150)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 脂肪由来幹細胞(ASC) / スフェロイド / 構造解析 / 共培養 / 単球(monocytes) / 脂肪由来幹細胞（ASC） / 三次元培養 / 単球(monocyte) / monocytes / ADSC |
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| Outline of Final Research Achievements |
Among potential cell-based therapies, adipose-derived stem cells (ASCs) have thought as a promising source of stem cells for tissue regeneration because it contains approximately 1000-fold more stem cells than the bone marrow.
In the present study, we analyzed the structures and expression of marker genes for multipotency, including Oct3/4, Nanog, and Sox2 of murine-derived ASC macrospheroids by in situ hybridization. The microstructures of the ASC macrospheroids were observed by transmission electron microscopy, and cell proliferation in the spheroids was analyzed by enzyme-linked immunoassay for bromodeoxyuridine as well as the growth and osteogenic potential of these macrospheroids in three-dimensional environment and following induction of calcification by co-culture with monocytes.
These results suggested that ASC macrospheroids presented high capacity for dynamic three-dimensional growth, and it might be promoted its osteogenic potential by monocytes.(Preparing for publication)
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