| Project/Area Number |
24659872
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKUDA Kazuhiro 新潟大学, 医歯学系, 准教授 (00169228)
NAGATA Masaki 新潟大学, 医歯学系, 准教授 (10242439)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 軟骨誘導 / 歯槽骨 / 骨膜細胞 / 培養 / 分化 |
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| Outline of Final Research Achievements |
We developed the method to prepare the periosteal sheet as as osteogenic grafting material from human alveolar periosteum and applied it in periodontal regenerative therapy. The purpose of this study is to develop a new technology of tissue-engineering cartilage from the periosteal sheet and to expand its clinical applicability.
Highly cell-multilayered periosteal sheets were detached, molded to be spherical and differentiated within a chondrocyte-differentiation medium. This treatment up-regulated chondrocyte markers, such as collagen II, proteoglycans, aggrecan, and Sox9 within the periosteal sphere. In contrast, collagen I was down-regulated and expressed only at and beneath the surface of the sphere. To the osteo-differentiation pathway, Wnt signals, the periosteal sphere was treated with a β-catenin inhibitor. However, it substantially suppressed cell viability and did not facilitate chondrogenic differentiation. It may be due to the nature of samples obtained from adult donors.
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