The development of culture surfaces expressing functional groups that enhance isolation, proliferation and differentiation of mesenchymal stem cells.

• Project/Area Number: 24659876
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KATO Yukio 広島大学, 医歯薬保健学研究院, 研究員 (10112062)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 間葉系幹細胞 / 培養皿 / 無血清培地 / 再生医療

Research Abstract

To improve adhesion in serum-free media, we developed self-assembled monolayer expressing OH and COOH at 60:40 (SAM-1). SAM-1 enhanced reorganization of adhesion plaques, adhesion and proliferation of human mesenchymal stem cells (MSC) in a serum-free medium. Furthermore, the expression of bone-related genes, along with calcium accumulation, was markedly enhanced in MSC cultures on SAM-1 after exposure to a serum-free osteogenesis-induction medium. In addition, SAM-1 enhanced anaerobic glycolysis and suppressed TCA cycle activity in MSC by down-regulating mitochondria-related-genes, which may be related to MSC stemness. These findings indicate that a combination of a serum-free medium and SAM is useful in basic studies on cell-substrate interactions and clinical studies towards MSC-based cell therapy.

Research Products

• [Journal Article] Seeding of mesenchymal stem cells into inner part of interconnected porous biodegradable scaffold by a new method with a filter paper (2014)
  • Author(s): Yamanaka K, Yamamoto K, Sakai Y, Suda Y, Shigemitsu Y, Kaneko T, Kato K, Kumagai T, Kato Y
  • Journal Title: Dental Materials Journal Volume: (in press)
  • NAID: 130004888175
  • Peer Reviewed
• [Journal Article] A novel protein, CHRONO, functions as a core component of the mammalian circadian clock. (2014)
  • Author(s): Goriki, A., Hatanaka, F., Myung J., Kim, J., Yoritaka, T., Tanoue, S., Abe, T., Kiyonari, H., Fujimoto, K., Kato, Y., Todo, T., Matsubara, A., Forger, D. and Takumi, T.
  • Journal Title: PLoS Biol. Volume: 12 Issue: 4 Pages: e1001839-e1001839
  • DOI: 10.1371/journal.pbio.1001839
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Seeding of mesenchymal stem cells into inner part of interconnected porous biodegradable scaffold by a new method with a filter paper. (2014)
  • Author(s): Yamanaka K, Yamamoto K, Sakai Y, Suda Y, Shigemitsu Y, Kaneko T, Kato K, Kumagai T, Kato Y.
  • Journal Title: Dental Materials Journal Volume: in press
  • NAID: 130004888175
  • Peer Reviewed
• [Journal Article] Different Circadian Expression of Major Matrix-Related Genes in Various Types of Cartilage : Modulation by Light-Dark Conditions (2013)
  • Author(s): Honda KK, Kawamoto T, Ueda HR, Nakashima A, Ueshima T, Yamada RG, Nishimura M, Oda R, Nakamura S, Kojima T, Noshiro M, Fujimoto K, Hashimoto S, Kato Y
  • Journal Title: J Biochem Volume: 154(4) Issue: 4 Pages: 373-81
  • DOI: 10.1093/jb/mvt068
  • NAID: 40019817581
  • Peer Reviewed
• [Journal Article] Age-dependent decrease in the chondrogenic potential of human bone marrow mesenchymal stem cells expanded with fibroblast growth factor-2 (2013)
  • Author(s): Kanawa M, Igarashi A, Ronald VS, Higashi Y, Kurihara H, Sugiyama M, Saskianti T, Pan H, Kato Y
  • Journal Title: Cytotherapy Volume: 15(9) Issue: 9 Pages: 1062-72
  • DOI: 10.1016/j.jcyt.2013.03.015
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells ameliorate experimental peritoneal fibrosis by suppressing inflammation and inhibiting TGF-beta1 signaling. (2013)
  • Author(s): Ueno, T., Nakashima, A., DOI:, S., Kawamoto, T., Honda, K., Yokoyama, Y., DOI:, T., Higashi, Y., Yorioka, N., Kato, Y., Kohno, N., and Masaki, T
  • Journal Title: Kidney Int Volume: 84(2) Issue: 2 Pages: 297-307
  • DOI: 10.1038/ki.2013.81
  • Peer Reviewed
• [Journal Article] Autologous Bone-Marrow Mesenchymal Stem Cell Implantation and Endothelial Function in a Rabbit Ischemic Limb Model (2013)
  • Author(s): Mikami S, Nakashima A, Nakagawa K, Maruhashi T, Iwamoto Y, Kajikawa M, Matsumoto T, Kihara Y, Chayama K, Noma K, Ochi M, Nishimura M, Tsuji K, Kato Y, Goto C, Higashi Y
  • Journal Title: PLOS ONE Volume: 8(7) Issue: 7 Pages: e67739-e67739
  • DOI: 10.1371/journal.pone.0067739
  • NAID: 120006986197
  • Peer Reviewed
• [Journal Article] ラット変形性関節症モデルでの高分子ヒアルロン酸応答遺伝子の解析 (2013)
  • Author(s): 加藤幸夫、本田清昌、中島歩、河本健
  • Journal Title: 臨床リウマチ Volume: 25(3) Pages: 174-184
• [Journal Article] The basic helix-loop-helix transcription factor DEC2 inhibits TGF-beta-induced tumor progression in human pancreatic cancer BxPC-3 cells (2012)
  • Author(s): Sato, F., Kawamura, H., Kawamoto, T., Noshiro, M.,(他10名)
  • Journal Title: Int J Mol Med Volume: 30 Issue: 3 Pages: 495-501
  • DOI: 10.3892/ijmm.2012.1037
  • Peer Reviewed
• [Journal Article] The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer (2012)
  • Author(s): Wu, Y., Kawamoto, T., Noshiro, M., et al.(他10名)
  • Journal Title: Int J Oncol Volume: 41 Issue: 4 Pages: 1337-1346
  • DOI: 10.3892/ijo.2012.1559
  • Peer Reviewed
• [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells (2012)
  • Author(s): Wu, Y., Sato, F., Bhawal, U.K., Kawamoto, T., Fujimoto, K., Noshiro, M., Seino, H., Morohashi, S., Kato, Y., Kijima, H
  • Journal Title: Biomedical Research Volume: 33 Issue: 2 Pages: 75-82
  • DOI: 10.2220/biomedres.33.75
  • NAID: 130004470811
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Regulation of basic helix-loop-helix transcription factors Dec1 and Dec2 by RORαand their roles in adipogenesis (2012)
  • Author(s): Ozaki N, Noshiro M, Kawamoto T, Nakashima A, Honda K, Fukuzaki-Dohi U, Honma S, Fujimoto K, Tanimoto K, Tanne K, Kato Y
  • Journal Title: Genes to Cells Volume: 17 Pages: 109-121
  • Peer Reviewed
• [Journal Article] Regulation of basic helix-loop-helix transcription factors Dec1 and Dec2 by RORα and their roles in adipogenesis. (2012)
  • Author(s): Ozaki N
  • Journal Title: Genes to Cells Volume: 17 Issue: 2 Pages: 107-121
  • DOI: 10.1111/j.1365-2443.2011.01574.x
  • Peer Reviewed
• [Journal Article] 循環器疾患における時計遺伝子DEC1の意義 (2012)
  • Author(s): 加藤幸夫他
  • Journal Title: Heart View Volume: 16 Pages: 103-107
• [Journal Article] Feasibility and limitations of the round robin test for assessment of in vitro chondrogenesis evaluation protocol in a tissue-engineered medical product (2011)
  • Author(s): Masako Yokoi, et al.
  • Journal Title: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE Volume: (掲載確定)(印刷中)) Issue: 7 Pages: 550-558
  • DOI: 10.1002/term.460
  • Peer Reviewed
• [Presentation] Isolation and expansion of mesenchymal stem cells from various tissues, including the umbilical cord, under serum-free conditions (2013)
  • Author(s): Yukio Kato, Jinchang Shao, Koichro Tsuji
  • Organizer: Asia CORD2013 April 19-20
  • Place of Presentation: in Kobe, Japan
• [Presentation] 無血清で作製した他家ヒト滑膜MSC(TEC)への期待 (2013)
  • Author(s): 加藤幸夫
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: 横浜市
• [Presentation] Isolation and expansion of mesenchymal stem cells from various tissues, including the umbilical cord, under serum-free conditions (2013)
  • Author(s): Yukio Kato, Jinchang Shao, Koichro Tsuji
  • Organizer: Asia CORD2013
  • Place of Presentation: 神戸市
  • Invited
• [Presentation] 無血清で作製した他家ヒト滑膜MSC（TEC）への期待 (2013)
  • Author(s): 加藤幸夫
  • Organizer: 第12回 日本再生医療学会
  • Place of Presentation: 横浜市
  • Invited
• [Presentation] 培養環境全体の化学的規定化による移植用間葉系幹細胞の増殖、分化の促進 (2012)
  • Author(s): 加藤幸夫
  • Organizer: (公)新化学技術推進協会ライフサイエンス技術部会・材料分科会
  • Place of Presentation: 東京都
  • Year and Date: 2012-09-18
• [Presentation] 膝関節症とヒアルロン酸への期待 (2012)
  • Author(s): 加藤幸夫、辻紘一郎
  • Organizer: 第2回TOKYOヘルスコレクション
  • Place of Presentation: 東京都
  • Year and Date: 2012-03-15
• [Presentation] 再生医療用細胞(MSCなど)の培養条件の完全化学規定化/至適化 (2012)
  • Author(s): 加藤幸夫
  • Organizer: 第11回再生医療の実用化に関するニーズ発表会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-02-24
• [Presentation] 概日リズムを制御する新規時計エレメントEL-boxの同定 (2012)
  • Author(s): 河本健、能城光秀、藤本勝巳、加藤幸夫
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福島県郡山市
• [Presentation] Chemical Optimization of Both Culture Surfaces and Media Markedly Enhances MSC Proliferation (2012)
  • Author(s): Yukio Kato, Tania Saskianti, Masami Kanawa, Takeshi Kawamoto, Koichi Kato, Isao Hirata
  • Organizer: ISSCR Annual Meeting
  • Place of Presentation: Yokohama, Japan
• [Presentation] 無血清培地で培養したヒト滑膜由来MSCの未分化性・軟骨分化能の検討 (2012)
  • Author(s): 中田恭輔、邵金昌、長谷川森一、杉田憲彦、下村和範、深澤賢宏、松本昌也、原真依子、河原裕美、弓削類、加藤幸夫、中村憲正、辻紘一郎
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: 横浜市
• [Presentation] 各組織由来MSCの均質性と細胞表面マーカーの発現に及ぼす無血清培地(STK培地)の影響 (2012)
  • Author(s): 長谷川森一、邵金昌、中村憲正、松下隆、金輪真佐美、鈴木美紀、原真依子、辻紘一郎、加藤幸夫
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: 横浜市
• [Presentation] 無血清培地(STK系列培地)を用いた間葉系幹細胞からのTissue Engineered Construct(TEC)作成 (2012)
  • Author(s): 邵金昌、長谷川森一、杉田憲彦、森口悠、下村和範、原真依子、桂由紀、坂井将典、河原裕美、弓削類、加藤幸夫、辻紘一郎、吉川秀樹、中村憲正
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: 横浜市
• [Presentation] Veronica Sainik Ronald, Katsuyuki Kozai, Yukio Kato Enhanced Proliferation of Stem Cells from Deciduous Teeth in Serum-free Media (2012)
  • Author(s): Noriko Goto, Katsumi Fujimoto, Shin-ichi Hasegawa, Kazuko Kitayama
  • Organizer: STK1/STK2 8th Biennial Conference PDAA(Pediatric Dentistry Association of Asia)
  • Place of Presentation: Bali , Indonesia 1st winner of scientific competition in poster presentation The best winner of scientific competition poster presentation
• [Presentation] Enhanced Proliferation of Stem Cells from Deciduous Teeth in Serum-free Media, STK1/STK2 (2012)
  • Author(s): Noriko Goto, et al.
  • Organizer: 8th Biennial Conference PDAA(Pediatric Dentistry Association of Asia)
  • Place of Presentation: Bali , Indonesia
• [Presentation] 無血清培地（STK系列培地）を用いた間葉系幹細胞からのTissue Engineered Construct(TEC)作成 (2012)
  • Author(s): 邵金昌他
  • Organizer: 第11回 日本再生医療学会
  • Place of Presentation: 横浜市
• [Presentation] 各組織由来MSCの均質性と細胞表面マーカーの発現に及ぼす無血清培地（STK培地）の影響 (2012)
  • Author(s): 長谷川森一他
  • Organizer: 第11回 日本再生医療学会
  • Place of Presentation: 横浜市
• [Presentation] 無血清培地で培養したヒト滑膜由来MSCの未分化性・軟骨分化能の検討 (2012)
  • Author(s): 中田恭輔他
  • Organizer: 第11回 日本再生医療学会
  • Place of Presentation: 横浜市
• [Presentation] 培養環境全体の化学的規定化による移植用間葉系幹細胞の増殖、分化の促進 (2012)
  • Author(s): 加藤幸夫
  • Organizer: （公）新化学技術推進協会ライフサイエンス技術部会・材料分科会
  • Place of Presentation: 東京都
  • Invited
• [Book] DEC1/STRA13/SHARP2 and DEC2/SHARP1 coordinate physiological processes, including circadian rhythms in response to environmental stimuli. bHLH Factors in Development and Disease: Current topics in developmental biology (2014)
  • Author(s): Kato Y, Kawamoto T, Fujimoto K, Noshiro M
  • Publisher: Elsevier(in press)
• [Book] bHLH Factors in Development and Disease: Current topics in developmental biology (2014)
  • Author(s): Kato Y, Kawamoto T, Fujimoto K, Noshiro M.
  • Publisher: Elsevier
• [Book] 幹細胞用無血清培地の開発、幹細胞医療の実用化技術と産業展望第3章幹細胞の維持培養と分化誘導 (2013)
  • Author(s): 加藤幸夫、邵金昌、長谷川森一、西村正宏、桂由紀、中村憲正、辻紘一郎
  • Total Pages: 293
  • Publisher: (株)シーエムシー出版刊(ISBN:978-4-7813-0692-6)
• [Book] 幹細胞医療の実用化技術と産業展望 (2013)
  • Author(s): 加藤幸夫、邵金昌、長谷川森一、西村正宏、桂由紀、中村憲正、辻紘一郎他
  • Total Pages: 293
  • Publisher: （株）シーエムシー出版
• [Book] 「幹細胞医療の実用化技術と産業展望」 幹細胞用無血清培地の開発 (2013)
  • Author(s): 加藤幸夫他
  • Total Pages: 293
  • Publisher: （株）シーエムシー出版