New development of clinical dental treatment that uses ceramics with cell compatibility and fish collagen

• Project/Area Number: 24659879
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: IKEDA Kahori 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (20578330)
• Co-Investigator(Kenkyū-buntansha): SUGIMOTO Kouji 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (40646113)
• Co-Investigator(Renkei-kenkyūsha): YOSHIZAWA Yuu 長崎大学, 病院(歯学系), 医員 (60746931)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: セラミックス / コラーゲン / 細胞移植 / 象牙質 / 再生医療 / フィッシュコラーゲン / セラミック坦体 / E-cad-Fcキメラタンパク質 / 象牙質再生 / 象牙芽細胞 / 硬組織再生 / fish collagen / 魚コラーゲン / 細胞毒性試験 / 感作性試験 / 皮内埋入試験

Outline of Final Research Achievements

Marine collagen derived fromfish scales, skin, and bone has been widely investigated for application as a scaffold and carrier due to its bioactive properties, including excellent biocompatibility, low antigenicity, and high biodegradability and cell growth potential.
This study was conducted to confirmthe safety of fish (tilapia) atelocollagen for use in clinical application.We performed in vitro and in vivo biological studies of medical materials to investigate the safety of fish collagen.The extract of fish collagen gel was examined to clarify its sterility. All present sterility tests concerning bacteria and viruses (including endotoxin) yielded negative results, and all evaluations of cell toxicity, sensitization, chromosomal aberrations, intracutaneous reactions, acute systemic toxicity, pyrogenic reactions, and hemolysis were negative.The present study demonstrated that atelocollagen prepared from tilapia is a promising biomaterial for use as a scaffold in regenerative medicine.

Research Products

• [Journal Article] Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold (2014)
  • Author(s): Takeshi Ikeda, Kahori Ikeda, Kouhei Yamamoto, Hidetaka Ishizaki, Yuu Yoshizawa,
  • Journal Title: BioMed Research International Volume: Vol2014
  • NAID: 120006986592
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fabrication and characteristics of chitosan sponge as a tissue engineering scaffold (2014)
  • Author(s): Takeshi Ikeda*, Kahori Ikeda, Kouhei Yamamoto, Hidetaka Ishizaki, Yuu Yoshizawa, Kajiro Yanagiguchi, Shizuka Yamada and Yoshihiko Hayashi
  • Journal Title: Biomedical Reserch of International Volume: ー
  • NAID: 120006986592
  • Peer Reviewed
• [Presentation] The Physicochemical Properties of Fish Collagen in Regeneration Medicine (2014)
  • Author(s): Ikeda T., Ikeda K, Yamamoto K., Yoshizawa y., Ikeda K., Yamada S., Hayashi Y.
  • Organizer: 7th IADR/PER
  • Place of Presentation: ドゥブロヴニク（クロアチア）
  • Year and Date: 2014-09-10 – 2014-09-13
• [Presentation] Properties of chitosan scaffold for pulp tissue engineering. (2013)
  • Author(s): Ikeda T, Ikeda K, Yamamoto K, Yoshizawa Y, Sugimoto K, Ishizaki H, Yamada S, Yanagiguch K, Hayashi Y
  • Organizer: 10th Asia Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago city(BigShip) , Tottori, Japan
• [Presentation] 足場材としての魚コラーゲンの安全性試験 (2013)
  • Author(s): 池田 毅，池田 香，吉澤 祐，柳口嘉治郎，山本耕平，山田志津香，林 善彦
  • Organizer: 第138回日本歯科保存学会春季大会
  • Place of Presentation: 福岡市（福岡国際会議場）
• [Presentation] 足場材としての魚コラーゲンの安全性試験 (2013)
  • Author(s): 池田 毅，池田 香，吉澤 祐，柳口嘉治郎，山本耕平，山田志津香，林 善彦
  • Organizer: 日本歯科保存学会2013年度春季学術大会
  • Place of Presentation: 福岡