| Project/Area Number |
24659889
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
OMURA KEN 東京医科歯科大学, 医歯学総合研究科, 教授 (10334434)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Keiichi 東京医科歯科大学, 硬組織疾患ゲノムセンター, 特任講師 (10396971)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 口腔がん / ゲノム / マイクロアレイ / 11q13.3 / マイクロダイセクション |
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| Research Abstract |
The aim is to develop the methods that detect the genomic structure abnormality in oral cancer cell with high-level genomic abnormality. We were confirming the satisfactory data of CGH microarray analysis using extracted 100ng input DNA from a formalin fixation paraffin embedded (FFPE) tissue. Moreover, we were confirming the satisfactory data of CGH microarray analysis using extracted DNA from a sample mounted in slide-glass which coverslip on the slide were removed by soaking the slide in xylene after immunohistostaining. To detect the genomic abnormality using smaller quantity of input DNA, we performed the targeted next-generation sequencing. We detected the genomic abnormality in oral cancer from 15ng input DNA.
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