Application of gene database for the treatment of cleft palate

• Project/Area Number: 24659890
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Osaka University
• Principal Investigator: SAKAI TAKAYOSHI 大阪大学, 歯学研究科(研究院), 教授 (90379082)
• Co-Investigator(Kenkyū-buntansha): NOHARA KANJI 大阪大学, 大学院歯学研究科, 准教授 (20346167)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 口蓋裂

Outline of Final Research Achievements

Our purpose of this study is to understand the mechanism of palatal fusion for cleft palate treatment. Gene expression database was created and applied for the discovery of genes that related to palatal fusion.Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) was identified from database and increased during and after palatal fusion. Quantitative real-time PCR confirmed the increase of CEACAM1. Functional antibody of CEACAM1 inhibited palatal fusion. CEACAM1 could be important for palatal fusion. These data regarding the interaction of CEACAM1 and palatal development would be used for future treatment of cleft palate.

Research Products

• [Journal Article] Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland. (2015)
  • Author(s): Islam MN, Itoh S, Yanagita T, Sumiyoshi K, Hayano S, Kuremoto K, Kurosaka H, Honjo T, Kawanabe N, Kamioka H, Sakai T, Ishimaru N, Taniuchi I, Yamashiro T
  • Journal Title: Dev Dyn. Volume: 244(3) Issue: 3 Pages: 488-496
  • DOI: 10.1002/dvdy.24231
  • Peer Reviewed / Open Access
• [Journal Article] Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells. (2015)
  • Author(s): Ono H, Obana A, Usami Y, Sakai M, Nohara K, Egusa H, Sakai T
  • Journal Title: Biomed Res Int. Volume: 2015 Pages: 293570-293570
  • DOI: 10.1155/2015/293570
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Melatonin Inhibits Embryonic Salivary Gland Branching Morphogenesis by Regulating Both Epithelial Cell Adhesion and Morphology. (2015)
  • Author(s): Obana-Koshino A, Ono H, Miura J, Sakai M, Uchida H, Nakamura W, Nohara K, Maruyama Y, Hattori A, Sakai T.
  • Journal Title: PLoS One Volume: 10(4) Issue: 4 Pages: e0119960-e0119960
  • DOI: 10.1371/journal.pone.0119960
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Videoendoscopic diagnosis for predicting the response to oral appliance therapy in severe obstructive sleep apnea. (2014)
  • Author(s): Sasao Y., Nohara K., Okuno K., Nakamura Y., Sakai T
  • Journal Title: Sleep and Breathing Volume: 18(4) Issue: 4 Pages: 809-815
  • DOI: 10.1007/s11325-014-0947-3
  • NAID: 130005405106
  • Peer Reviewed
• [Journal Article] The efficacy of a lingual augmentation prosthesis for swallowing after a glossectomy: A clinical report. (2014)
  • Author(s): Okuno, K., Nohara K., Tanaka N., Sasao Y., Sakai T
  • Journal Title: Journal of Prosthetic Dentistry Volume: 111(4), Issue: 4 Pages: 342-345
  • DOI: 10.1016/j.prosdent.2013.08.011
  • Peer Reviewed
• [Journal Article] Regulation of the Epithelial Adhesion Molecule CEACAM1 Is Important for Palate Formation (2013)
  • Author(s): Junko Mima, Aya Koshino, Kyoko Oka, Hitoshi Uchida, Yohki Hieda, Kanji Nohara, Mikihiko Kogo, Yang Chai, Takayoshi Sakai
  • Journal Title: PLoS ONE Volume: 8 Issue: 4 Pages: e61653-e61653
  • DOI: 10.1371/journal.pone.0061653
  • Peer Reviewed
• [Journal Article] Effects of the bolusvolume on hyoid movements in normal individuals. (2013)
  • Author(s): Ueda, N., Nohara, K., Kotani, Y., Tanaka, N., Okuno, K., Sakai, T.
  • Journal Title: Journal of Oral Rehabilitation Volume: 40(7) Issue: 7 Pages: 491-499
  • DOI: 10.1111/joor.12060
  • Peer Reviewed
• [Journal Article] Influences of swallowing volume and viscosity on regulation of levator veli palatini muscle activity during swallowing. (2013)
  • Author(s): Okuno, K., Tachimura, T., Sakai, T.
  • Journal Title: Journal of Oral Rehabilitation Volume: 40(9) Issue: 9 Pages: 657-663
  • DOI: 10.1111/joor.12071
  • Peer Reviewed
• [Journal Article] Btbd7/Cleftin regulates cleft formation and branching morphogenesis of epithelial cells. (2013)
  • Author(s): Sakai, T., Onodera, T.
  • Journal Title: Journal of Oral Biosciences Volume: 55(2) Issue: 2 Pages: 73-75
  • DOI: 10.1016/j.job.2013.03.002
  • Peer Reviewed
• [Journal Article] Swallowing frequency in elderly people during daily life. (2013)
  • Author(s): Tanaka, N., Nohara, K., Kotani, Y., Matsumura, M., Sakai, T.
  • Journal Title: Journal of Oral Rehabilitation Volume: 40(10) Issue: 10 Pages: 744-750
  • DOI: 10.1111/joor.12085
  • NAID: 130007843698
  • Peer Reviewed
• [Journal Article] Development of a swallowing frequency meter using a laryngeal microphone (2012)
  • Author(s): Tanaka N, Nohara K, Okuno K, Kotani Y, Okazaki H, Matsumura M, Sakai T
  • Journal Title: Journal of Oral Rehabilitation Volume: (印刷中) Issue: 6 Pages: 411-420
  • DOI: 10.1111/j.1365-2842.2012.02293.x
  • Peer Reviewed
• [Journal Article] 口腔内装置治療により睡眠時無呼吸症候群に伴う心室性期外収縮が改善した１症例 (2012)
  • Author(s): 奥野健太郎、佐々生康宏、野原幹司、中村祐己、阪井丘芳
  • Journal Title: 日本口腔科学会雑誌 Volume: 61 Pages: 332-336
  • NAID: 10031144865
  • Peer Reviewed
• [Journal Article] 高齢者施設入所者の食事支援における食事リスク判定タグの試み (2012)
  • Author(s): 金子信子、野原幹司、浅埜正人、阪井丘芳、光山誠
  • Journal Title: 老年歯科医学 Volume: 27 Pages: 18-24
  • NAID: 10031137192
  • Peer Reviewed
• [Presentation] Models of salivary gland differentiation using induced pluripotent stem (iPS) cells. (2015)
  • Author(s): Ono H, Obana A, Usami Y, Sakai M, Miura J, Egusa H, Sakai T
  • Organizer: 47th Meeting of Continental European Division of the International Association for Dental Research
  • Place of Presentation: Antalya (Turkey)
  • Year and Date: 2015-09-14
  • Int'l Joint Research
• [Presentation] The change in coordination of wallowing and respiration in sleep stages. (2015)
  • Author(s): Okuno k, Nohara k, Sakai T, Lowe AA, Almeida FR
  • Organizer: American Thoracic Society 2015 International Conference
  • Place of Presentation: Colorado (USA)
  • Year and Date: 2015-05-15
  • Int'l Joint Research
• [Presentation] The relationship between CEACAM1 and TGFβ in palatal fusion. (2013)
  • Author(s): Sakai, T., Koshino, A., Ono, H., Uchida, H., Mima, J., Hieda, Y., Nohara, K., Koga, M., Chai, Y., Oka, K.
  • Organizer: 12th international congress on cleft lip/palate and related craniofacial anomalies.
  • Place of Presentation: Florida, USA
• [Presentation] A comparison of the prevalences of speech sound errors in Japanese children with or without cleft palate. (2013)
  • Author(s): Sugiyama, C., Nohara, K., Takai, E., Sakai, T.
  • Organizer: 12th international congress on cleft lip/palate and related craniofacial anomalies.
  • Place of Presentation: Florida, USA
• [Presentation] An investigation of relationship between articulation and motor coordination in individuals with cleft palate (2013)
  • Author(s): Sugiyama, C., Nohara, K., Hikage, A., Miyamoto, K., Koshino, A., Okuno, K., Sakai, T.
  • Organizer: 12th international congress on cleft lip/palate and related craniofacial anomalies.
  • Place of Presentation: Florida, USA
• [Presentation] Videofluorographic study of velar velocity during speech and swallowing in adults with/without cleft palate. (2013)
  • Author(s): Takai, E., Nohara, K., Sato, K., Sugiyama, C., Sakai, T.
  • Organizer: 12th international congress on cleft lip/palate and related craniofacial anomalies.
  • Place of Presentation: Florida, USA
• [Presentation] Cephalometric analysis of patients with complete unilateral cleft lip and palate following the early two-stage palatoplasty. (2013)
  • Author(s): Otsuki, K., Yamanishi, T., Yamamoto, T., Fujibayashi, E., Hiroishi, S., Tsuji, T., Seikai, T., Nohara, K., Sakai, T., Kogo, M.
  • Organizer: 12th international congress on cleft lip/palate and related craniofacial anomalies.
  • Place of Presentation: Florida, USA
• [Presentation] 口蓋癒合時におけるCeacam1とTGFβ3との関連について (2012)
  • Author(s): 越野綾、内田仁司、美馬淳子、古郷幹彦、野原幹司、檜枝洋記、岡暁子、阪井丘芳
  • Organizer: 第36回日本口蓋裂学会総会・学術大会
  • Place of Presentation: 京都
• [Presentation] 非口蓋裂児における異常構音の発言に関する研究 (2012)
  • Author(s): 杉山千尋、野原幹司、尾島麻希、奥野健太郎、若杉葉子、宮本勝行、日景朱美、越野綾、阪井丘芳
  • Organizer: 第36回日本口蓋裂学会総会・学術大会
  • Place of Presentation: 京都
• [Presentation] Furlow法とpush-back法による口蓋形成術後の補綴装置装着について (2012)
  • Author(s): 高井英月子、野原幹司、越野綾、佐藤耕一、杉山千尋、山西整、古郷幹彦、阪井丘芳
  • Organizer: 第36回日本口蓋裂学会総会・学術大会
  • Place of Presentation: 京都
• [Presentation] The Potential Role of Ceacam1 in Folic Acid-Related Mouse Embryonic Palatal Fusion (2012)
  • Author(s): Sakai, T., Mima, J., Koshino, A., Uchida, H., Kogo, M., Nohara, K., Hieda, Y.
  • Organizer: The 69th Annual Meeting of American Cleft Palate-craniofacial Association
  • Place of Presentation: California
• [Presentation] Videofluorographic Study of Velar Velocity during Speech in Adults with/without Cleft Palate (2012)
  • Author(s): Takai, E., Nohara, K., Satoh, K., Sugiyama, C., Sakai, T.
  • Organizer: The 69th Annual Meeting of American Cleft Palate-craniofacial Association
  • Place of Presentation: California
• [Book] 摂食・嚥下機能改善と装置の作り方超入門 (2013)
  • Author(s): 阪井丘芳、前田芳信
  • Total Pages: 96
  • Publisher: クインテッセンス出版株式会社