The analysis of molecular mechanism of bone destruction using invitro cocultivation assay and application to a risk management in ameloblastoma
Project/Area Number |
24659900
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Surgical dentistry
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Research Institution | Kagoshima University |
Principal Investigator |
FUJII Tomomi 鹿児島大学, 医歯(薬)学総合研究科, 教務職員 (90305152)
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Co-Investigator(Kenkyū-buntansha) |
KISHIDA Shosei 鹿児島大学, 医歯学総合研究科, 教授 (50274064)
SENBA Ichiro 鹿児島大学, 医歯学総合研究科, 教授 (60145505)
NAKAMURA Norifumi 鹿児島大学, 医歯学総合研究科, 教授 (60217875)
KOMATSUZAWA Hitoshi 鹿児島大学, 医歯学総合研究科, 教授 (90253088)
KIBE Toshiro 鹿児島大学, 医学部歯学部附属病院, 医員 (50635480)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 国際情報交換 / インドネシア / エナメル上皮腫 / 分子生物学 |
Research Abstract |
In this study, we immortalized cells which we obtained from the lesion of ameloblastoma and the oral mucosa of the healthy subject and established new ameloblastoma cell line (AM-3) and normal oral mucosa epithelium cell line (MOE-1). The expression of Wnt5a, Fz-2, MMP-2, -9 was higher remarkably in ameloblastoma cell line than normal oral mucosa epithelium cell line. Also, Wnt-3a treatment activated expression of MMP-9 of AM-3. Furthermore, osteoclasts differentiation was induced when we cocultivated with AM-3cells and RAW 264.7 cells which were osteoclastic precursors.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Ryk is essential for Wnt-5a-dependent invasiveness in human glioma2014
Author(s)
Habu M, Koyama H, Kishida M, Kamino M, Iijima M, Fuchigami T, Tokimura H, Ueda M, Tokudome M, Koriyama C, Hirano H, Arita K, Kishida S
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Journal Title
J Biochem
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining.2013
Author(s)
Hirano H, Yonezawa H, Yunoue S, Habu M, Uchida H, Yoshioka T, Kishida S, Kishida M, Oyoshi T, Fujio S, Sugata S, Yamahata H, Hanaya R, Arita K.
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Journal Title
Brain Tumor Pathology
Volume: 31
Issue: 2
Pages: 85-93
DOI
Related Report
Peer Reviewed
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[Journal Article] A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis2013
Author(s)
Kibe T, Fuchigami T, Kishida M, Iijima M, Ishihata K, Hijioka H, Miyawaki A, Semba I, Nakamura N, Kiyono T, Kishida S
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Journal Title
Oral Surg Oral Med Oral Pathol Oral Radiol
Volume: 115(6)
Issue: 6
Pages: 780-8
DOI
Related Report
Peer Reviewed
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[Journal Article] A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis2013
Author(s)
Toshiro Kibe, Takao Fuchigami, Michiko Kishida, Mikio Iijima, Hiroshi Hijioka, Akihiko Miyawaki, Ichiro Semba, Norifumi Nakamura, Tohru Kiyono, and Shosei Kishida
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Journal Title
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Volume: 116
Pages: 1-9
Related Report
Peer Reviewed
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