| Project/Area Number |
24659907
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHITA Nobuo 東北大学, 大学院歯学研究科, 助教 (50431515)
IKEDA Etsuko 東北大学, 大学院歯学研究科, 大学院非常勤i市 (20509012)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ヒト・エピソーマルiPS細胞 / 遺伝子導入 / 再生医学 / 歯科 |
|---|
| Research Abstract |
In the present study, we established human episormal iPS cells to develop fundamental technologies for artificial tooth regeneration. We generated human episormal iPS cells derived from periodontal ligament cells, dental papilla cells, and dental follicle cells. We tested teratoma formation of dental pulp cell-derived iPS cells. As a result, tissues derived from three germ layers were observed. We performed transfection of Pax9-expression plasmid into neral crest-like cells (NCLCs) derived form murine iPS cells. The expression of DMP1 was upregulated in the Pax9-overexpressing NCLCs. Moreover, NCLC sphere showed induction of mRNA expressions of transcription factors that were expressed in mesenchymal cells of tooth germs.
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