The autoantibody effects of periodontopathic bacteria on threatened preterm labor and preterm birth.

• Project/Area Number: 24659921
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: IZUMI Yuichi 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60159803)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Hiroaki 東京医科歯科大学, 医歯学総合研究科, 助教 (50396967) ; TAKEUCHI Yasuo 東京医科歯科大学, 医歯学総合研究科, 助教 (60396968) ; KATAGIRI Sayaka 東京医科歯科大学, 医歯学総合研究科, 助教 (60510352)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 歯周病 / 早産 / 早期低体重児 / 早期低体重児出産 / 切迫早産 / 自己抗体 / 抗リン脂質抗体

Research Abstract

Periodontal disease is considered to be a risk factor for threatened preterm labor (TPL) and adverse delivery outcomes such as preterm birth and low birth weight (PLBW). Oral plaque and saliva were taken from 28 women diagnosed as TPL; a further 36 control samples were acquired from healthy pregnant women. Placenta samples were taken after delivery. Periodontopathic bacteria were identified by real-time PCR with TaqMan probe. Serum IgG antibodies against periodontopathic bacteria were measured by ELISA
Of 64 births, 13 delivered PLBW. Serum anti-P.gingivalis IgG antibody titer, plaque P.gingivalis levels, plaque T.forsythia level and saliva P.intermedia levels were significantly higher in the PLBW group compared with controls. Between TPL and healthy control group, there was no significant difference.
This study suggested that oral infection with P.gingivalis, T.forsythia and P.intermedia, and high level of anti-P.gingivalis IgG antibody titer are risk factors in PLBW.

Research Products

• [Journal Article] The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth (2013)
  • Author(s): Ye CC, Katagiri S, Miyasaka N, Bharti P, Kobayashi H, Takeuchi Y, Momohara Y, Sekiguchi M, Takamine S, Nagasawa T, and Izumi Y
  • Journal Title: Archives of Gynecology and Obstetrics Volume: 288 Issue: 1 Pages: 65-72
  • DOI: 10.1007/s00404-013-2741-z
  • Peer Reviewed
• [Journal Article] Application of quantitative proteomic analysis using tandem mass tags for discovery and identification of novel biomarkers in periodontal disease. (2013)
  • Author(s): Tsuchida S, Satoh M, Kawashima Y, Sogawa K, Kado S, Sawai S, Nishimura M, Ogita M, Takeuchi Y, Kobyashi H, Aoki A, Kodera Y, Matsushita K. Izumi Y. Nomura F
  • Journal Title: Proteomics Volume: Vol.13 Issue: 15 Pages: 2339-2350
  • DOI: 10.1002/pmic.201200510
  • Peer Reviewed
• [Journal Article] Effect of glycemic control on periodontitis in type 2 diabetic patients with periodontal disease (2013)
  • Author(s): Katagiri S, Nitta H, Nagasawa T, Izumi Y, Kanazawa M, Matsuo A, Chiba H, Fukui M, Nakamura N, Oseko F, Kanamura N, Inagaki K, Noguchi T, Naruse K, Matsubara T, Miyazaki S, Miyauchi T, Ando Y, Hanada N, Inoue S
  • Journal Title: J Diabetes Investig Volume: 4(3) Pages: 320-325
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/23997922
• [Journal Article] Effect of glycemic control on periodontitis in type 2 diabetic patients with periodontal disease. (2013)
  • Author(s): Katagiri S, Inagaki K, et al
  • Journal Title: J Diabetes Invest Volume: 4 Issue: 3 Pages: 1-6
  • DOI: 10.1111/jdi.12026
  • Peer Reviewed
• [Presentation] 切迫早産と低体重児出産における歯周病原細菌とその抗体価に関する研究 (2014)
  • Author(s): 御給美沙、片桐さやか、小林宏明、竹内康雄、池田裕一、須田智也、須藤毅顕、和泉雄一
  • Organizer: 第140回日本歯科保存学会春季学術大会大津
  • Place of Presentation: 滋賀
• [Presentation] 切迫早産と低体重児出産における歯周病原細菌とその抗体価に関する研究 (2014)
  • Author(s): 御給美沙、片桐さやか、小林宏明、竹内康夫、池田裕一、須田智也、須藤毅顕、和泉雄一
  • Organizer: 第140回日本歯科保存学会春季学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール、滋賀
• [Presentation] Periopathogenic Bacteria in Oral Plaque (2013)
  • Author(s): Ye C, Katagiri S, Bharti P, Kobayashi H, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: Saliva and Placenta from PLBW. International Academy of Dental Research
  • Place of Presentation: Seattle USA
• [Presentation] Periopathogenic bacteria in oral plaque, saliva and placenta from preterm low birth weight cases (2013)
  • Author(s): Ye C, Katagiri S, Bharti P, Kobayashi H, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: 10th Asian Pacific Society of Periodontology meeting
  • Place of Presentation: Nara, Japan
• [Presentation] Periopathogenic bacteria in oral plaque, saliva and placenta from preterm low birth weight cases. (2013)
  • Author(s): Changchang Ye,Hiroaki Kobayashi,Sayaka Katagiri,Yasuo Takeuchi,Toshiyuki Nagasawa,Yuichi lzumi
  • Organizer: 10th Asian Pacific Society of Periodontology meeting.
  • Place of Presentation: Nara Prefectural New Public Hall, Nara, Japan
• [Presentation] Periopathogenic Bacteria in Oral Plaque, Saliva and Placenta from PLBW (2013)
  • Author(s): C. YE, H. KOBAYASHI, P. BHARTI, S. KATAGIRI, Y. TAKEUCHI, T. NAGASAWA, and Y. IZUMI
  • Organizer: IADR
  • Place of Presentation: Seattle USA
• [Presentation] The role of antibodies against periodontal pathogen on preterm birth (2012)
  • Author(s): Ye C, Katagiri S, Bharti P, Kobayashi H, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: 98th Annual meeting American Academy of periodontology collaboration with the Japanese society of periodontology
  • Place of Presentation: Los Angeles, USA
• [Presentation] The role of antibodies against periodontal pathogen on preterm birth (2012)
  • Author(s): Changchang Ye, Sayaka Katagiri, Pariksha Bharti, Hiroaki Kobayashi, Yasuo Takeuchi, Toshiyuki Nagasawa, Yuichi Izumi
  • Organizer: 98th Annual meeting American Academy of periodontology collaboration with the Japanese society of periodontology.
  • Place of Presentation: Los Angeles,USA
• [Presentation] The role of periodontal bacteria on antiphospholipid syndrome and preterm low birth weigh
  • Author(s): Changchang Ye, Sayaka Katagiri, Pariksha Bharti, Hiroaki Kobayashi, Yasuo Takeuchi, Toshiyuki Nagasawa, Yuichi Izumi.
  • Organizer: 日中歯科大会
  • Place of Presentation: 成都、中国
• [Presentation] 早産・低体重児出産における歯周病に起因した b2glycoprotein-I を認識する抗体の役割
  • Author(s): Changchang Ye, 片桐さやか, Pariksha Bharti, 小林宏明, 竹内康雄、長澤敏行, 和泉雄一
  • Organizer: 第55回日本歯周病学会春季学術大会
  • Place of Presentation: 札幌コンベンションセンター