| Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2012: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Outline of Final Research Achievements |
In the usual contextual fear conditioning, the animals have two types of learning [(1) learning the context (CS) and, (2) learning the association between the context (CS) and the shock (US)]. Because these types of learning co-occur during conditioning, it is difficult to identify brain regions required for CS-US association.
In this study, I performed Arc CatFISH method to detect brain regions required for the CS-US association and found that parietal association cortex (PtA) responded to CS-US signals. To test whether PtA regulates CS-US association at behavioral level, I established new behavioral paradigms to measure CS, US, and CS-US independently. By performing this behavioral paradigm with injection of sodium channel blocker lidocaine, I found that PtA regulates CS-US association. Furthermore I found that, by optogenetics technique, manipulating the PtA activity is capable of generating an artificial CS-US associative memory.
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