TRIC channels and calcium release from endo/sarcoplasmic reticulum

• Project/Area Number: 24680041
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kyoto University
• Principal Investigator: YAMAZAKI Daiju 京都大学, 生理化学研究ユニット, 特定講師 (40467428)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000); Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2012: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
• Keywords: TRICチャネル / 小胞体 / カウンターイオン / 平滑筋 / カルシウムスパーク / 過分極シグナル / TRIC-A / カルシウム放出 / 血管平滑筋 / 血圧調節 / リアノジン受容体 / IP3受容体

Outline of Final Research Achievements

In this study, it was revealed the following three points using smooth muscle cells having both TRIC channel subtypes and Ca2+ release channels including ryanodine cereptor (RyR) and inositol-1,4,5-trisphosphate receptor (IP3R). 1) Overexpression of TRIC-A enhanced hyperpolarization signaling which evoked by Ca2+ spark, and showed hypotension induced by constitutively vascular relaxation. 2) It was suggested that TRIC-A expression level in vascular smooth muscle cells controlled blood pressure. 3) It was suggested that TRIC-A expression level in vascular smooth muscle cells also affected the amount of Ca2+ contents in the endo/sarcoplasmic reticulum which distributed RyR and/or IP3R.

Research Products

• [Journal Article] Contribution of calumin to embryogenesis through participation in the endoplasmic reticulum-associated degradation activity. (2014)
  • Author(s): Yamamoto S, Yamazaki T, Komazaki S, Yamashita T, Osaki M, Matsubayashi M, Kidoya H, Takakura N, Yamazaki D, Kakizawa S
  • Journal Title: Dev Biol Volume: 393 Issue: 1 Pages: 33-43
  • DOI: 10.1016/j.ydbio.2014.06.024
  • Peer Reviewed
• [Journal Article] Trimeric intracellular cation channels and sarcoplasmic/endoplasmic reticulum calcium homeostasis (2014)
  • Author(s): Zhou X, Lin P, Yamazaki D, Park KH, Komazaki S, Chen SR, Takeshima H, Ma J
  • Journal Title: Circ Res Volume: 114 Issue: 4 Pages: 706-716
  • DOI: 10.1161/circresaha.114.301816
  • Peer Reviewed
• [Journal Article] TRIC channel and hypertension (2013)
  • Author(s): Yamazaki D, Tao S, Takeshima H.
  • Journal Title: Clin Calcium Volume: 23 Pages: 543-550
• [Journal Article] Facilitated hyperpolarization signaling in vascular smooth muscle overexpressing TRIC-A channels. (2013)
  • Author(s): Tao, S., Yamazaki, D., Komazaki, S., Zhao, C., Iida, T., Kakizawa, S., Imaizumi, Y. & Takeshima, H.
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 22 Pages: 15581-15589
  • DOI: 10.1074/jbc.m112.435396
  • Peer Reviewed
• [Journal Article] TRIC channels supporting efficient Ca2t release from intracellular stores (2013)
  • Author(s): Venturi E, Sitsapesan R, Yamazak i D, Takeshima H
  • Journal Title: Pflugers Archives Volume: 465 Pages: 187-195
  • Peer Reviewed
• [Journal Article] TRIC-B channels display labile gating : evidence from the TRIC-A knockout mouse model (2013)
  • Author(s): Venturi E, MatyJaszkiewicz A,Pitt SJ. Tsaneva-Atanasova K, Nishi M. Yamazaki D, et al.
  • Journal Title: Pflugers Archives Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] The Roles of Calcium and Phosphorus in Cardiovascular Diseases. TRIG channel and hypertension (2013)
  • Author(s): Yamazaki D, Tao S, Takeshima H.
  • Journal Title: Clinical Calcium Volume: 23 Pages: 543-550
• [Journal Article] Facilitated hyperpolarization signaling in vascular smooth muscle overexpressing TRIC-A channels (2013)
  • Author(s): Tao S, Yamazaki D, Komazaki S, et al.
  • Journal Title: Journal of Biological Chemistry Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] TRIC (trimeric intracellular cation)-A channels contribute to blood pressuremaintenance in vascular smooth muscle (2012)
  • Author(s): Yamazaki D
  • Journal Title: Yakugaku Zasshi Volume: 132 Pages: 1037-1043
  • Peer Reviewed
• [Presentation] 小胞体カウンターイオンチャネルによる細胞内カルシウム制御 (2015)
  • Author(s): 山崎大樹、竹島浩
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] 血圧調節におけるカウンターイオンチャネルの機能的役割の解明 (2014)
  • Author(s): 山崎大樹、竹島浩
  • Organizer: 心血管膜輸送研究会2014
  • Place of Presentation: 岡崎・生理学研究所
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] TRICチャネルによる小胞体Ca2+分布 (2014)
  • Author(s): 山崎大樹、陶晟辰、竹島浩
  • Organizer: 第56回日本平滑筋学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-08-06 – 2014-08-08
• [Presentation] The MBOAT family protein Mitsugumin 56 contributes to postnatal maturation in the mouscle sarcoplasmic reticulum (2014)
  • Author(s): Bo Van, Miyuki Nishi, Shinji Komazaki, Ki Ho Park, Daiju Yamazaki, Jianjie Ma, Hiroshi Takeshima
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco
• [Presentation] Impaired bone formation in Tric-b-knockout mice (2014)
  • Author(s): Chengzhu Zhao, Daiju Yamazaki, Fumiyo Aoyama, Tsunaki Iida, Miyuki Nishi, Akira Sawaguchi, Hiroshi Takeshima
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco
• [Presentation] H+ inhibits TRIC-B channels derived from mouse TRIC-A knockout tissue (2014)
  • Author(s): Fiona OBrien, Elisa Venturi, Elena Galfre, Antoni Matyjaszkiewicz, Daiju Yamazaki, Miyuki Nishi, Hiroshi Takeshima, Rebecca Stisapesan
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco
• [Presentation] SR counter-ion channels mediated blood pressure regulation (2014)
  • Author(s): Daiju Yamazaki, Shengchen Tao, Hiroshi Takeshima
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
  • Invited
• [Presentation] TRIC-A欠損マウスにおけるイソプロテレノール誘発性心臓線維化 (2014)
  • Author(s): 山崎大樹、趙成珠、陶晟辰、王朝弘、西美幸、竹島浩
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台
• [Presentation] TRIC-A過剰発現による血管弛緩メカニズム (2014)
  • Author(s): 山崎大樹、陶晟辰、竹島浩
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] TRIC-Aは血管平滑筋においてSRのカルシウム分布を制御する (2013)
  • Author(s): 山崎大樹、陶晟辰、竹島浩
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2013-03-22
• [Presentation] Co-expression of TRIC-A and cardiac Ryanodine receptor affects store-overloadinduced calcium release in HEK293 cells (2013)
  • Author(s): Zhou X, Park K-H, Lin p-H, Yamazaki D, et al.
  • Organizer: Biophysical Society 57th Annual Meeting
  • Place of Presentation: Philadelphia (アメリカ)
  • Year and Date: 2013-02-06
• [Presentation] Hypotension in SM-specific TRIC-A-transgenic mice (2013)
  • Author(s): Tao S, Yamazaki D, et al.
  • Organizer: Biophysical Society 57th Annual Meeting
  • Place of Presentation: Philadelphia (アメリカ)
  • Year and Date: 2013-02-04
• [Presentation] The role of Ca_<2+> handling in TRIC knockout neonatal cardiomyocytes (2013)
  • Author(s): Zhao C, Park K-H, Naitou D, Wang C Yamazaki n, et al.
  • Organizer: Biophysical Society 57th Annual Meeting
  • Place of Presentation: Philadelphia (アメリカ)
  • Year and Date: 2013-02-04
• [Presentation] TRIC-Aの過剰発現による過分極シグナルの亢進は血管を弛緩させる (2013)
  • Author(s): 陶 晟辰、山崎 大樹、竹島 浩
  • Organizer: 第8回トランスポーター研究会
  • Place of Presentation: 熊本
• [Presentation] Facilitated hyperpolarization signaling relaxes vascular smooth muscle in Tric-a-overexpressing mice (2013)
  • Author(s): Daiju Yamazaki
  • Organizer: HDP第2回国際シンポジウム
  • Place of Presentation: 東京
• [Presentation] 平滑筋特異的TRIC-A過剰発現マウスにおける低血圧発症メカニズムの解明 (2013)
  • Author(s): 陶 晟辰、山崎 大樹、駒崎 伸二、趙 成珠、飯田 綱規、柿澤 昌、今泉 祐治、竹島 浩
  • Organizer: 第123回日本薬理学会近畿部会
  • Place of Presentation: 名古屋
• [Presentation] 平滑筋特異的TRIC-A過剰発現マウスにおける低血圧発症メカニズム (2013)
  • Author(s): 陶 晟辰、山崎 大樹、竹島 浩
  • Organizer: 第63回日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都
• [Presentation] 平滑筋特異的TRIC-Aトランスジェニツクマウスにおける過分極シグナルの亢進 (2012)
  • Author(s): 山崎大樹、陶晟辰、竹島浩
  • Organizer: 筋生理の集い
  • Place of Presentation: 東京慈恵会医科大学(東京都)
  • Year and Date: 2012-12-22
• [Presentation] 血管平滑筋におけるTRIC-Aチャネルによる血圧調節メカニズムの解明 (2012)
  • Author(s): 山崎大樹、陶晟辰、喜多紗斗美, 他
  • Organizer: 第26回日本薬学会近畿支部総会・大会
  • Place of Presentation: 武庫川女子大学浜甲子園キャンパス(兵庫県)(招待講演)
  • Year and Date: 2012-10-20
• [Presentation] 循環機能調節における小胞体カルシウムハンドリング (2012)
  • Author(s): 山崎大樹
  • Organizer: 京都大学学際融合教育研究推進センター 生理化学研究ユニットシンポジウム
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2012-06-13
• [Presentation] TRICチャネルによる血圧調節メカニズム (2012)
  • Author(s): 山崎大樹
  • Organizer: 第7回トランスポーター研究会
  • Place of Presentation: 京都大学(京都府)(招待講演)
  • Year and Date: 2012-06-10